| List of Antibodies tested on mouse tissue samples | Fixatives and antibody dilutions tested | |||||||||||||||||||||||
| Antigen | Antigen Species | Antibody type | Antibody Species | Provider | Product No. | Batch | Antigen retrieval | Dilution range tested | Fekete's | Zn | Bouin's | NBF | PFA | Recommended control tissue | ||||||||||
| 15-lipoxygenase | Human | monoclonal, 061402DM | Rabbit | Oxford Biomedical Research | LX25 | NO | 50-8,000 | 50 | Hair follicles | Legend | ||||||||||||||
| a-gp27 Gag | Viral (MMTV) | monoclonal, Blue-7-1 | Mouse | The Jackson Laboratory, Dr. Tatyana Golovkina | Citrate (5 cycles) | 1-10 | 5 | MMTV-infected mammary gland | Staining too pale | |||||||||||||||
| a-gp36 Env | Viral (MMTV) | monoclonal, Blue 6-1 | Mouse | The Jackson Laboratory, Dr. Tatyana Golovkina | NO | 1-10 | 10 | MMTV-infected mammary gland | Background too high | |||||||||||||||
| a-gp52 Env | Viral (MMTV) | monoclonal, Black 6-5D | Mouse | The Jackson Laboratory, Dr. Tatyana Golovkina | NO | 1-10 | 5 | MMTV-infected mammary gland | No signal | |||||||||||||||
| Alpha actinin | Chicken | Rabbit | Sigma | A2543 | 78F-4865 | 100-2,000 | Good signal | |||||||||||||||||
| Alpha catenin | Human (recombinant) | Rabbit | Chemicon | AB19021 | Citrate (5 cycles) | 250-1,000 | 250 | Intestinal epithelium | Not tested | |||||||||||||||
| Alpha catenin | Human/Mouse (synthetic peptide) | Rabbit | Sigma | C2081 | Citrate (5 cycles) | 250-5,000 | 250 | 250 | 250 | Intestinal epithelium | ||||||||||||||
| Alpha SHP-1 | monoclonal, D-11 | Rabbit | Santa Cruz | sc-7289 | 10-50 | |||||||||||||||||||
| Alpha smooth muscle actin | Human (synthetic peptide) | monoclonal, 1A4 | Mouse | Sigma | A2547 | NO | 6,000-40,000 | 15000 | 15000 | 15000 | 15000 | 15000 | Smooth muscle fibers, myoepithelial cells | |||||||||||
| Alpha-fetoprotein | Human | Rabbit | Dako | A0008 | 097 | 1,000-5,000 | ||||||||||||||||||
| Alpha-Sarcomeric Actin | Rabbit | monoclonal, 5C5 | Mouse | Sigma | A2172 | NO | 5,000-20,000 | Myocardium and skeletal muscles; least background with Zinc at 1:10,000 | ||||||||||||||||
| Androgen receptor (AR; N20) | Human (recombinant) | Rabbit | Santa Cruz | sc-816 | A2003 | Citrate (5 cycles) | 100 | 200 | 100 | 200 | 200 | Leydig cells (testis) | ||||||||||||
| Angiopoietin 3 (M18) | Mouse (recombinant) | Goat | Santa Cruz | sc-9355 | ||||||||||||||||||||
| Angiopoietin 3 (R19) | Mouse (recombinant) | Goat | Santa Cruz | sc-9357 | ||||||||||||||||||||
| ARF (H50) | Human (recombinant) | Rabbit | Santa Cruz | sc-9063 | B221 | 10-500 | ||||||||||||||||||
| Bax | Human (recombinant) | Rabbit | R&D Systems | AF820 | ||||||||||||||||||||
| Bcl2 | Human (recombinant) | monoclonal, C-2 | Mouse | Santa Cruz | sc-492 | C1403 | 25-450 | |||||||||||||||||
| bcl-2 | Mouse (recombinant) | Goat | R&D Systems | AF810 | ||||||||||||||||||||
| Bcl-6 (N3) | Human (recombinant) | Rabbit | Santa Cruz | sc-858 | H201 | EDTA (Catoretti) | 25-500 | 25 | 25 | Lymphoid follicles | ||||||||||||||
| Beta catenin | Human (recombinant) | monoclonal, NA | Mouse | Chemicon | MAB2081 | Citrate (5 cycles) | 100-2,000 | Colon | ||||||||||||||||
| Beta catenin | Human (recombinant) | Rabbit | Chemicon | AB19022 | Citrate (5 cycles) | 250-1,000 | 500 | 250 | 500 | 500 | Intestinal epithelium | |||||||||||||
| Beta-catenin | Human/Mouse (synthetic peptide) | Rabbit | Sigma | C2206 | Citrate (5 cycles) | 250-5,000 | 250 | 250 | 250 | Colonic epithelium | ||||||||||||||
| Beta-catenin (C18) | Human (recombinant) | Goat | Santa Cruz | sc-1496 | B1803 | NO | 450 | 150 | 300 | 150 | 150 | Colon, duodenum, cornea, esophagus, epidermis | ||||||||||||
| Bgp1 | Mouse | Rabbit | Dr. Beauchemin | Variable | 2,000-10,000 | 10000 | 5,000 -10,000 | 5,000 | 5,000 -10,000 | 5,000 -10,000 | Glands with ducts and digestive tract | |||||||||||||
| Bgp2 | Mouse | Rabbit | Dr. Beauchemin | Citrate (5 cycles) | 2,000-10,000 | 2000 | 2000 | 2000 | Colon | |||||||||||||||
| BrdU | monoclonal, Bu20a | Mouse | Dako | M0744 | 033 (Aug.96) | Trypsin/HCl/Sodium Borate | 20 | 20 | Epidermis, duodenum | |||||||||||||||
| BrdU, conjugated to KLH | monoclonal, BU-33 | Rat | Sigma | B2531 | Trypsin/HCl/Sodium Borate | 40 | 40 | Epidermis, duodenum | ||||||||||||||||
| BVP-1 | Bovine papilloma virus type I | Rabbit | Dako | B0580 | NO | 2000 | 2000 | Viral papillomas | ||||||||||||||||
| Caspase 3 | Human (recombinant) | Rabbit | R&D Systems | AF835 | ||||||||||||||||||||
| Caspase 3 (CPP32) | Human (recombinant) | monoclonal, 4-1-18 | Mouse | Chemicon | MAB4703 | 23021221 | 25-750 | 25 | Small intestine | |||||||||||||||
| Cathepsin D | Human | monoclonal, C5 | Mouse | Chemicon | MAB422 | 22120520 | 100-400 | |||||||||||||||||
| CC26 (Clara cell protein 26 kD) | Rat | polyclonal | Rabbit | Chemicon | AB3700 | 23030909 | Variable | 1600 (citrate) | 800 (citrate) | 1600 | 800 | 1600 | Lung (also labels a variety of other tissues) | |||||||||||
| CCR3 | Mouse (synthetic peptide) | Rabbit | BD Biosciences | 556882 | 200-1,000 | |||||||||||||||||||
| CD106 | Mouse | monoclonal, 429 | Rat (IgG2b) | The Jackson Laboratory (Ted Duffy) | TJL186B | 10-50 | ||||||||||||||||||
| CD106 (VCAM-1) | Mouse | monoclonal, M/K-2 | Rat | Chemicon | CBL1300 | 176U0266 | 25-500 | |||||||||||||||||
| CD146 | Human | monoclonal, P1H12 | Mouse | Chemicon | MAB16985 | 21121094 | 100-1,000 | |||||||||||||||||
| CD19 | Mouse | monoclonal, ID3 | Rat (IgG2b) | The Jackson Laboratory (Ted Duffy) | TJL167B | 10-50 | ||||||||||||||||||
| CD22 | Mouse | monoclonal, 2D6 | Rat | Chemicon | CBL1311 | 1311451723 | 50 | 50 | 50 | 50 | 50 | Spleen, lymph nodes | ||||||||||||
| CD28 (M-20) | Mouse (recombinant) | Goat | Santa Cruz | sc-1624 | K198 | 25-500 | ||||||||||||||||||
| CD29 | Mouse | monoclonal, OXM718 | Hamster | Chemicon | CBL1333 | 50-1000 | ||||||||||||||||||
| CD3 | Mouse | monoclonal, 17A2 | Rat | Chemicon | CBL1302 | 13023893 | 100-1000 | |||||||||||||||||
| CD3 (PC3/188A) | Human | monoclonal, PC3/188A | Mouse | Santa Cruz | sc-20047 | I1102 | 25-1000 | |||||||||||||||||
| CD31 (PECAM-1) | Mouse | monoclonal, MEC13.3 | Rat | BD Biosciences | 550274 | 10-100 | ||||||||||||||||||
| CD38 | Mouse | monoclonal, 90 | Rat | Chemicon | CBL1338 | 1338516143 | NO | 50-500 | 50 | B cells (spleen and Peyer's patches), thymic medulla | ||||||||||||||
| CD3-d (M-20) | Mouse (recombinant) | Goat | Santa Cruz | sc-1129 | F010 | EDTA (Catoretti) | 25 | 25 | Paracortical areas in lymph nodes, Periarteriolar lymphoid sheaths in spleen, thymus (scattered cells) | |||||||||||||||
| CD3e | Mouse | Rat | The Jackson Laboratory | TJL 154 P | 20-500 | |||||||||||||||||||
| CD3e | Mouse | monoclonal, 145-2C11 | Hamster | The Jackson Laboratory (Ted Duffy) | TJL010B | 10-50 | ||||||||||||||||||
| CD3-e | Mouse | monoclonal, KT3 | Rat | Chemicon | CBL1317 | 21080415 | 100-1000 | |||||||||||||||||
| CD3-gamma (C-18) | Mouse (recombinant) | Goat | Santa Cruz | sc-1125 | F010 | NO | 25 | Intra-epithelial T cells of duodenum | ||||||||||||||||
| CD4 | Unknown | monoclonal, GK1.5 | Rat | Chemicon | CBL1305 | 130561P | 20-500 | |||||||||||||||||
| CD4 | Mouse | monoclonal, YTS191.1 | Rat | The Jackson Laboratory | TJL 099P | 100-500 | ||||||||||||||||||
| CD4 (C-18) | Human (recombinant) | Goat | Santa Cruz | sc-1140 | J3002 | 10-200 | ||||||||||||||||||
| CD40 (C-20) | Human (recombinant) | Goat | Santa Cruz | sc-1731 | J3102 | 10-500 | ||||||||||||||||||
| CD45 | Mouse | monoclonal, C363.16A | Rat | Chemicon | CBL1343 | 1343227113 | NO | 50-500 | 250 | 250 | 250 | 50 | Intraepithelial T cells in intestine, thymocytes in thymic medulla, periarteriolar lymphoid sheaths; also works on whole gastro-intestinal mounts | |||||||||||
| CD45R/B220 | Mouse | monoclonal, RA3-6B2 | Rat | Chemicon | CBL1342 | 22010573 | Variable | 25-500 | 50 (no AgR) | 125 (with AgR), 50 (w/o AgR) | 100 (AgR) | 100 (AgR) | 100 (AgR) | Lymphoid tissues | ||||||||||
| CD49d | Mouse | monoclonal, R1-2 | Rat | BD Biosciences | 553154 | 100-2,000 | ||||||||||||||||||
| CD62L | Mouse | monoclonal, MEL-14 | Rat | Chemicon | MAB2151Z | 23030949 | Citrate (5 cycles) | 25 | 25 | 25 | 25 | |||||||||||||
| CD68 (H-255) | Human (recombinant) | Rabbit | Santa Cruz | sc-7084 | G101 | Citrate (5 cycles) | 400 | 400 | 400 | 200 | 200 | Small intestine (scattered epithelial cells) | ||||||||||||
| CD83 (FL-205) | Human (recombinant) | Rabbit | Santa Cruz | sc-20083 | E132 | 10-200 | ||||||||||||||||||
| CD8a | Mouse | monoclonal, YTS169.4 | Rat | The Jackson Laboratory | TJL O98P | 20-500 | ||||||||||||||||||
| CD8a | Mouse | monoclonal, 53-6.72 | Rat | The Jackson Laboratory | TJL 032 P | 20-500 | ||||||||||||||||||
| cdk1/p34cdc2 | Xenopus | monoclonal, A17.1.1 | Mouse | Chemicon | MAB8878 | 18100662 | Citrate (5 cycles) | 50-500 | ||||||||||||||||
| CDK2 | Human | Mouse | BD Biosciences | 610145 | 1,000-10,000 | |||||||||||||||||||
| CDK4 | Human (recombinant) | monoclonal, DCS-35 | Mouse | Chemicon | MAB8879 | 22110362 | Citrate (5 cycles) | 100-500 | ||||||||||||||||
| Cdk4 (C-22) | Mouse (recombinant) | Rabbit | Santa Cruz | sc-260 | A1403 | 10-300 | ||||||||||||||||||
| C-myc (9E10) | Human (recombinant) | Rabbit | Santa Cruz | sc-262 | B2603 | 10-300 | ||||||||||||||||||
| Collagen IV | Mouse | Rabbit | Chemicon | AB756P | Variable | 20-100 | 100 | Kidney | ||||||||||||||||
| Collagen IV | Human | monoclonal, IV-4H12 | Mouse | Chemicon | MAB3326 | 50-4500 | ||||||||||||||||||
| Collagen IV | Mouse | Rabbit | Chemicon | AB756 | YES | 500-2,500 | 500 | 500 | 500 | Basement membranes | ||||||||||||||
| Cyclin A | Human (recombinant) | Rabbit | Rockland | 100-400-151 | 2626 | 100-2,000 | ||||||||||||||||||
| Cyclin A (H-432) | Human (recombinant) | Rabbit | Santa Cruz | sc-751 | L1602 | EDTA (Catoretti) | 50 | 50 | 25 | 25 | 25 | Thymus | ||||||||||||
| Cyclin B1 | Human (recombinant) | Rabbit | Rockland | 100-401-152 | 2627 | 100-2,000 | ||||||||||||||||||
| Cyclin D1 | Human (recombinant) | Rabbit | Rockland | 100-401-153 | 11756 | 100-2,000 | ||||||||||||||||||
| Cyclin D1 (72-13G) | Mouse (recombinant) | monoclonal, 72-13G | Mouse | Santa Cruz | sc-450 | G252 | EDTA (Catoretti) | 100 | Tumors (no labeling in non-neoplastic tissues) | |||||||||||||||
| Cyclin D3 (C-16) | Human (recombinant) | Rabbit | Santa Cruz | sc-182 | C1803 | EDTA (Catoretti) | 25-100 | 100 | 100 | Thymus | ||||||||||||||
| Cyclin E | Human (recombinant) | Rabbit | Upstate | 06-459 | 100-2,000 | |||||||||||||||||||
| Cytokeratin 15 | Mouse | monoclonal, LHK15 | Mouse | Chemicon | CBL272 | 1508U0015 | 5 | 5 | 5 | 5 | 5 | Haired skin | ||||||||||||
| Desmin | Human | monoclonal, DE-B-5 | Mouse | Chemicon | IHC2011-6 | 22090574 | Citrate (5 cycles) | 1 | 1 | 1 | 1 | 1 | Smooth muscles and striated skeletal muscles; also artifactually labels the transitional epithelium, the cornea, salivary glands, and mammary luminal cells with some fixatives | |||||||||||
| DVN-1 | Mouse | Mouse | Dr. Derry Roopenian | NO | Labeling with many fixatives (1:100) and in frozen tissues in wt and transgenic mouse | |||||||||||||||||||
| EGF | Mouse | Rabbit | Sigma | E2635 | NO | 1,000-5,000 | 2000 | 1000 | 2000 | Ducts of salivary glands, epidermis | ||||||||||||||
| EGFR | Human (synthetic peptide) | monoclonal | Mouse | Sigma | E3138 | Citrate (5 cycles) | 500-5,000 | 500 | 500 | Axones of peripheral nerve | ||||||||||||||
| Eosinophil peroxidase | Human | monoclonal | Mouse | Chemicon | MAB1087 | 21080539 | Citrate (5 cycles) | 50-500 | 150 | skin of cpdm mice | ||||||||||||||
| Eosinophilic major basic protein | Unknown | monoclonal, AHE-2 | Mouse | Chemicon | MAB1086 | 21120015 | 50-500 | |||||||||||||||||
| Eosinophilic major basic protein | Human | monoclonal, BMK13 | Mouse | Chemicon | CBL419 | 4192705P | 50-500 | |||||||||||||||||
| Ephrin B1 (C-18) | Human recombinant (identical to mouse ephrin B1 and B2) | Rabbit | Santa Cruz | sc-910 | A0803 | Citrate (5 cycles) | 100 | 100 | 50 | 200 | 100 | Colon and duodenum (scattered epithelial cells) | ||||||||||||
| ERB-4 (C-18) | Human (recombinant) | Rabbit | Santa Cruz | sc-283 | C0603 | Citrate (5 cycles) | 800 | 600 | 600 | 600 | Nuclear labeling of the cells of the crypts of small intestine, cardiomyocytes, neurones | |||||||||||||
| erbB2 | Rat | monoclonal, 42 | Mouse | BD Biosciences | 610161 | 1,000-5,000 | ||||||||||||||||||
| Estrogen receptor (aa120-170) | Bovine | monoclonal, AER304 | Mouse | Chemicon | MAB443 | 50-500 | ||||||||||||||||||
| F4/80 (macrophages) | Mouse | Rat | The Jackson Laboratory | TJL O46P | 50-500 | |||||||||||||||||||
| Factor VIII-related antigen | Human | Mouse | Dako | M0616 | 093 | 50-1000 | ||||||||||||||||||
| Fas/TNFRSF6 (CD95) | Mouse (recombinant) | Goat | R&D Systems | AF435 | ||||||||||||||||||||
| Fibronectin | Human | monoclonal, FN-15 | Mouse | Sigma | F7387 | 100-2,000 | ||||||||||||||||||
| Filaggrin | Mouse | Rabbit | Covance | PRB-417P | NO | 5,000-35,000 | 15000 | 10000 | 10000 | 5000 | 5000 | Stratum corneum | ||||||||||||
| Flk1 | Mouse (recombinant) | monoclonal, A3 | Mouse | Santa Cruz | sc-6251 | 100-2,000 | ||||||||||||||||||
| Gamma catenin | Human (recombinant) | monoclonal, 11 E4 | Mouse | Chemicon | MAB2083 | Citrate (5 cycles) | 100-2,000 | |||||||||||||||||
| Glial fibrillary acidic protein (GFAP) | Human | Rabbit | Sigma | G9269 | Citrate (5 cycles) | 100-7,500 | 7500 | 2500 | Glial cells | |||||||||||||||
| Ha1 | Human | Mouse | Dr. Fleckman | 1-50 | ||||||||||||||||||||
| Ha2 | Human | Mouse | Dr. Fleckman | 1-50 | ||||||||||||||||||||
| Ha5 | Human | Mouse | Dr. Fleckman | 1-50 | ||||||||||||||||||||
| Hb1 | Human | Mouse | Dr. Fleckman | 1-50 | ||||||||||||||||||||
| IgG4 | Human | monoclonal, G17-4 | Mouse | BD Biosciences | 555882 | 30238 | 50-130 | |||||||||||||||||
| IL7R-alpha | Mouse | monoclonal, A7R34 | Rat | eBioscience | 14-1271 | 100 | 100 | 100 | Granulocytes | |||||||||||||||
| Integrin alpha 6 | Unknown | monoclonal, MA6 | Rat | Chemicon | MAB1982 | 25-1,000 | ||||||||||||||||||
| Integrin beta 1 | Unknown | monoclonal, MB1.2 | Rat | Chemicon | MAB1997 | Citrate (5 cycles) | 50-1,000 | 1000 | Intestine | |||||||||||||||
| Involucrin | Mouse | Rabbit | Covance | PRB-142C | NO | 4,000-10,000 | 7500 | 7500 | 7500 | Epidermis | ||||||||||||||
| IRF-4 (M-17) | Mouse (recombinant) | Goat | Santa Cruz | sc-6059 | J0802 | EDTA (Catoretti) | 25 | 25 | 50 | |||||||||||||||
| JAK1 | Human/Mouse (recombinant) | Rabbit | Upstate | 06-272 | Citrate (5 cycles) | 100-2,000 | 100 | 100 | 500 | Gastric epithelium epidermis | ||||||||||||||
| JAK2 | Mouse (recombinant) | Rabbit | Upstate | 06-255 | Citrate (5 cycles) | 100-2,000 | 100 | 100 | Urothelium., most epithelia | |||||||||||||||
| Keratin 1 | Mouse | Rabbit | Covance | PRB-165P | NO | 6,000-25,000 | 12000 | 6000 | 12000 | Epidermis | ||||||||||||||
| Keratin 1 | Human | Mouse | Dr. Fleckman | 1-25 | Haired skin | |||||||||||||||||||
| Keratin 10 | Mouse | Rabbit | Covance | PRB-159P | NO | 2,500-15,000 | 2500 | 2500 | 2500 | 2500 | Epidermis | |||||||||||||
| Keratin 10 | Human | monoclonal, DE-K10 | Mouse | Dr. Fleckman, NeoMarkers | MS-611 | Citrate (5 cycles) | 50-1,000 | 200 | 50 | 50 | Haired skin | |||||||||||||
| Keratin 13 | Human | monoclonal, Ks 13.1 | Mouse | Chemicon | CBL176 | NO | 100-500 | 100 | 200 | 100 | 100 | Epidermis | ||||||||||||
| Keratin 13 | Human | monoclonal, KS-1A3 | Mouse | Sigma | C0791 | 50-1,000 | ||||||||||||||||||
| Keratin 14 | Mouse | Rabbit | Covance | PRB-155P | NO | 6,000-25,000 | 25000 | 12000 | 12000 | 25000 | 6000 | Epidermis | ||||||||||||
| Keratin 14, C-terminus | Human (recombinant) | monoclonal, LL002 | Mouse | Dr. Fleckman, NeoMarkers | MS-115 | Citrate (5 cycles) | 50-500 | 50 | 100 | 50 | 50 | Oral mucosa, haired skin | ||||||||||||
| Keratin 15, C-terminus | Human (recombinant) | monoclonal, LHK15 | Mouse | Dr. Fleckman | MS-1068 | Citrate (5 cycles) | 5-500 | 5 | 5 | 5 | Haired skin | |||||||||||||
| Keratin 16, C-terminus | Human (recombinant) | monoclonal, LL025 | Mouse | Dr. Fleckman | MS-620 | Digestion | 1-500 | 1 | Oral mucosa, haired skin | |||||||||||||||
| Keratin 17 | Rat | monoclonal | Mouse | Chemicon | MAB1677 | 1-200 | ||||||||||||||||||
| Keratin 17 | Rat | monoclonal, E3 | Mouse | Dr. Fleckman, NeoMarkers | MS-489 | 50-1,000 | Cornea, epidermis, mammary gland | |||||||||||||||||
| Keratin 17 | Mouse (recombinant) | Rabbit | Dr. Pierre Coulombe | NO | 750-2,500 | 2,500 | 750 | 2,500 | 2,500 | 2,500 | Hair follicles; also labels the wall of some arteries | |||||||||||||
| Keratin 18 | Human | monoclonal, Ks18.04 | Mouse | Chemicon | CBL177 | 100-2,000 | ||||||||||||||||||
| Keratin 18 | Human | monoclonal, DC10 | Mouse | Chemicon | MAB1600 | 100-2,000 | ||||||||||||||||||
| Keratin 18 | Unknown | monoclonal, RGE53 | Mouse | Chemicon | MAB3234 | Digestion | 100-2,000 | 500 | 200 | 500 | Ducts and ductules in exocrine pancreas and salivary glands | |||||||||||||
| Keratin 18 | Human | monoclonal, M9 | Mouse | Monosan | MON 3006 | 10-50 | ||||||||||||||||||
| Keratin 20 | Human | monoclonal, Ks 20.8 | Mouse | Chemicon | CBL208 | 50-1,000 | ||||||||||||||||||
| Keratin 3/12 | Rabbit | monoclonal, AE5 | Mouse | Chemicon | CBL218 | 21812101p-01 | Digestion | 50-500 | 200 | 200 | 200 | 200 | Haired skin | |||||||||||
| Keratin 5 | Mouse | Rabbit | Covance | PRB-160P | NO | 7,000-30,000 | 7000 | 7000 | 7000 | Epidermis | ||||||||||||||
| Keratin 6 | Mouse | Rabbit | Covance | PRB-169P | NO | 6,000-25,000 | 12000 | 6000 | 6000 | 6000 | Epidermis | |||||||||||||
| Keratin 6, C-terminus | Human (recombinant) | monoclonal, LHK6B | Mouse | Dr. Fleckman, NeoMarkers | MS-766 | Citrate (5 cycles) | 5-500 | Haired skin | ||||||||||||||||
| Keratin 6a | Guinea pig | Dr. Roop | K6-212 | Citrate (5 cycles) | 500-2,000 | 500 | 500 | Oral mucosa, haired skin | ||||||||||||||||
| Keratin 6a/b | Rabbit | Dr. Roop | K6-83 | Citrate (5 cycles) | 500-2,000 | 500 | 500 | 1000 | 500 | 500 | Haired skin | |||||||||||||
| Keratin 6b | Rabbit | Dr. Roop | K6-82 | Citrate (5 cycles) | 250-2,000 | Oral mucosa, haired skin | ||||||||||||||||||
| Keratin 7 | Human | monoclonal, LP5K | Mouse | Chemicon | CBL194 | NO | 25 | 25 | Urothelium | |||||||||||||||
| Keratin 7 | Human | monoclonal, LdS68 | Mouse | Chemicon | CBL184 | 50-1,000 | ||||||||||||||||||
| Keratin 7 | Unknown | monoclonal, RCK105 | Mouse | Monosan | MON 3007 | Digestion | 25-1,000 | 50 | Outer root sheath; does not label the structures it is supposed to label (transitional epithelium) | |||||||||||||||
| Keratin 8 | Human | monoclonal, LP3K | Mouse | Chemicon | CBL195 | 100-500 | ||||||||||||||||||
| Keratin 8 | Human | monoclonal, NA | Mouse | Chemicon | MAB1634 | 100-2,000 | ||||||||||||||||||
| Keratin 8 | Human | monoclonal, LP3K | Mouse | Chemicon | CBL195 | 100-2,000 | ||||||||||||||||||
| Keratin 8 | monoclonal, EL41 | Potoroo | Dr. E.B. Lane | 5 | ||||||||||||||||||||
| Keratin 8, 18 | Bovine | Guinea pig | Progen | GP11 | Citrate (5 cycles) | 300-600 | 450 | 450 | 600 | Colonic epithelium; also labels structures it is not supposed to label | ||||||||||||||
| Keratin 8/18 | monoclonal, EL61 | Potoroo | Dr. E.B. Lane | 5 | ||||||||||||||||||||
| Keratins type I | Unknown | monoclonal, AE1 | Mouse | Chemicon | CBL215 | Variable | 100-500 | 200 | 200 | 150 (AgR) | Haired skin | |||||||||||||
| Keratins type II | Human | monoclonal, AE3 | Mouse | Chemicon | MAB1611 | NO | 50-1,000 | 50 | 50 | 50 | Haired skin; does not labell all structures it is supposed to label | |||||||||||||
| Keratins 4, 5, 6, 8, 10, 13, 18 | Human | monoclonal, C11 | Mouse | Chemicon | CBL234 | Digestion | 50-1,000 | 50 | Does not label all structures it is supposed to label | |||||||||||||||
| Keratins 4, 5, 6, 8, 10, 13, 18 | Unknown | monoclonal | Mouse | Chemicon | MAB1636 | NO | 50-1,200 | 1000 | 1000 | 1000 | Haired skin | |||||||||||||
| Keratins 5, 14 | Human | monoclonal, LH8 | Mouse | Chemicon | CBL267 | 25-1,000 | ||||||||||||||||||
| Keratins 5, 6 | Human | monoclonal, D5/16 B4 | Mouse | Chemicon | MAB1620 | Citrate (5 cycles) | 50-200 | 100 | 50 | Haired skin | ||||||||||||||
| Keratins 5, 8 | Human | monoclonal | Mouse | Chemicon | MAB3228 | NO | 50-1,000 | 250 | 250 | 250 | 250 | PFA is great for the CL of the ORS; discrepencies between the fixatives | ||||||||||||
| Keratins 5, 8 | Unknown | monoclonal | Mouse | Chemicon | MAB1629 | Digestion | 50-1,000 | 500 | 500 | 500 | 500 | Esophagus, tongue; does not label all structures it is supposed to label | ||||||||||||
| Keratins 7, 17 | Unknown | monoclonal | Mouse | Chemicon | MAB1625 | 50-1,750 | Haired skin, oral mucosa | |||||||||||||||||
| Keratins 8, 18 | Human | monoclonal, NCL-5D3 | Mouse | Chemicon | CBL170 | Digestion | 25-500 | 25 | 25 | Colonic epithelium | ||||||||||||||
| Ki67 (MKI67) | Human | monoclonal, B56 | Mouse | BD Biosciences | 550609 | Citrate (5 cycles) | 25-100 | 25 | 25 | Intestinal epithelium, lymphoid follicles | ||||||||||||||
| Ki-67 (MKI67) | Human (recombinant) | monoclonal | Mouse | Invitrogen | 08-0156 | |||||||||||||||||||
| Ki67 (Mki67, M-19) | Mouse (recombinant) | Goat | Santa Cruz | sc-7846 | A3103 | Citrate (5 cycles) | 200 | 200 | 200 | 200 | 200 | Thymus | ||||||||||||
| Laminin | Mouse | Rabbit | Sigma | L9393 | NO | 3,000-15,000 | 3000 | 3000 | Basement membrane of skeletal muscle fibers and blood vessels | |||||||||||||||
| Loricrin | Mouse | Rabbit | Covance | PRB-145P | NO | 4,000-15,000 | 8000 | 8000 | 8000 | 8000 | Epidermis | |||||||||||||
| L-selectin (CD62L) | Mouse | monoclonal, MEL-14 | Rat | Chemicon | MAB2151Z | 21031198 | 25-750 | |||||||||||||||||
| M30 CytoDEATH (cytokeratin 18) | Human | monoclonal, M30 | Mouse | Roche | 2 140 322 | |||||||||||||||||||
| Mac-1 (CD11b) | Mouse | monoclonal | Rat (IgG2b) | The Jackson Laboratory, Ted Duffy | TJL063B | 10-50 | ||||||||||||||||||
| Major Basic Protein | Mouse | Rabbit | Dr. Elizabeth Lenkiewicz | Rabbit 509 (10/16/98) | Digestion 1.5 min | 2000-4000 | 2000 | cpdm mouse tissues | ||||||||||||||||
| MHC Class II (Ia) | Mouse | monoclonal, M5/114 | Rat | The Jackson Laboratory, Ted Duffy | 250 | 250 | 250 | 250 | 250 | 250 | ||||||||||||||
| NCAM (CD56) | Chicken | Rabbit | Chemicon | AB5032 | 23010388 | 650 | 650 | 650 | 650 | 650 | ||||||||||||||
| NCAM (CD56) | Mouse | Rat | Chemicon | MAB310 | 23020285 | Citrate (5 cycles) | 100 | 200 | 50 | 50 | 50 | |||||||||||||
| Neurofilament | Bovine | Rabbit | Sigma | N4142 | NO | 3,500-15,000 | 7000 | Axones of peripheral nerves, neurones | ||||||||||||||||
| Neuron-specific enolase (NSE) | Bovine | Rabbit | Dako | A0587 | NO | 4,000-15,000 | 15000 | 8000 | Peripheral nerves, ganglion cells | |||||||||||||||
| NK | Mouse | monoclonal, Dx5 | Rat | The Jackson Laboratory | TJL 148 | 20-500 | ||||||||||||||||||
| NK1.1 cells | Mouse | monoclonal, PK136 | Mouse | The Jackson Laboratory | TJL 83 | 20-500 | ||||||||||||||||||
| NLDC 145 (dendritic cells) | Mouse | Rat | The Jackson Laboratory | TJL 122 | 20-500 | |||||||||||||||||||
| NOS2 | Human (recombinant) | Rabbit | Santa Cruz | sc-651 | 750 | 1000 | 1000 | 1000 | 1250 | Dendritic cells in thymus (nuclear) | ||||||||||||||
| ODC | Mouse (recombinant) | monoclonal, ODC-29 | Mouse | Sigma | O1136 | 3,500-15,000 | Corneal epithelium shows mild diffuse labelling whenever available: artifact? Possible artifactual labeling of epithelial cells of the colon, brush border of proximal tubules of the kidney, etc at a dilution of 1:750 with AgR (Bouin's and Fekete's) | |||||||||||||||||
| p15 (M-20) | Mouse (recombinant) | Goat | Santa Cruz | sc-1429 | D101 | Variable | 100 | 100 | 100 (citrate buffer) | Bone marrow | ||||||||||||||
| p16 (H-156) | Human (recombinant) | Rabbit | Santa Cruz | sc-1207 | K281 | 50-200 | ||||||||||||||||||
| p19 (M-167) | Mouse (recombinant) | Rabbit | Santa Cruz | sc-1063 | E170 | 50-200 | ||||||||||||||||||
| p21 | Mouse (recombinant) | monoclonal, F-5 | Mouse | Santa Cruz | sc-6246 | B1003 | 100 | Mammary tumors in mice transgenic for myc and wnt1 | ||||||||||||||||
| p27/Kip1 (C19) | Human recombinant) | monoclonal, C-19 | Rabbit | Santa Cruz | sc-528 | C1303 | Citrate (5 cycles) | 25 | 25 | 25 | 25 | 25 | Small intestine; Bouin's is the best but labeling is only moderate and there is background | |||||||||||
| p53 | Human (recombinant) | monoclonal, DO-7 | Mouse | BD Biosciences | 550831 | Citrate (5 cycles) | 25-100 | 25 | Some tumors | |||||||||||||||
| p63 | Human (recombinant) | monoclonal, 4A4 | Mouse | Chemicon | MAB4135 | 2212133 | NO | 250-750 | 750 | 750 | 750 | 750 | 750 | Transtional epithelium, male accessory sexual glands | ||||||||||
| p63 | Human recombinant | monoclonal, 4A4 | Mouse | Santa Cruz | sc-8431 | L0302 | EDTA (Catoretti) | 25 | 25 | 10 | 25 | 50 | Basal layers of esophagus, epidermis, mammary gland, prostate, vagina, etc | |||||||||||
| Pancadherin | Human (synthetic peptide) | Rabbit | Sigma | C3678 | NO | 1,000-100,000 | 5000 | 5000 | 5000 | 5000 | 5000 | Epidermis, colon, placenta | ||||||||||||
| Pax | Mouse (recombinant) | Rabbit | Covance | PRB-278P | 500-1,000 | |||||||||||||||||||
| PCNA | Mouse (recombinant) | monoclonal, PC10 | Mouse | Santa Cruz | sc-56 | A0903 | Variable | 500 | 500 (citrate) | 750 | 100 | 500 | Duodenum, thymus | |||||||||||
| PECAM 1 (M-20) | Mouse (recombinant) | Goat | Santa Cruz | sc-1506 | 25-500 | Works on whole mounts (endothelial cells) at 1:400 | ||||||||||||||||||
| PECAM-1 | Mouse | monoclonal, 390 | Rat | Chemicon | CBL1337 | 133715882P | 25-750 | |||||||||||||||||
| Phospho-beta-catenin (Ser33/37Thr41) | Human (synthetic) | Rabbit | Cell Signaling | 9561 | NONE | |||||||||||||||||||
| Phospho-beta-catenin (Thr41/Ser45) | Human (synthetic) | Rabbit | Cell Signaling | 9565 | NONE | |||||||||||||||||||
| P-selectin | Mouse | Rabbit | Chemicon | CBL40 | 40161P-01 | EDTA (Catoretti) | 50 | 50 | 50 | 50 | 25 | Spleen and bone marrow (megakaryocytes, platelets) | ||||||||||||
| Rb (C-15) | Human (recombinant) | Rabbit | Santa Cruz | sc-50 | B0503 | 25-1000 | ||||||||||||||||||
| Rel B | Mouse (recombinant) | Rabbit | Santa Cruz | sc-226 | C2803 | 500 | 500 | 1000 | 1000 | 1000 | Nucleus of some dendritic cells and M cells of Peyer's patches; slightly better with citrate buffer antigen retrieval | |||||||||||||
| S100 | Bovine | monoclonal, 15E2E2 | Rabbit | Chemicon | MAB079 | 22011259 | Citrate (5 cycles) | 1,000-20,000 | Axones of peripheral nerves, cartilage, neurones | |||||||||||||||
| S100 | Bovine | Mouse | Sigma | S2644 | 100-500 | |||||||||||||||||||
| Shh, C-terminus | Mouse (recombinant) | Goat | R&D Systems | AF445 | ||||||||||||||||||||
| Shh, N-terminus | Mouse (recombinant) | Goat | R&D Systems | AF464 | ||||||||||||||||||||
| TGF-beta | Human (recombinant) | Rabbit | Sigma | T9429 | NO | 500-10,000 | 1000 | 1000 | Epidermis | |||||||||||||||
| Thy1.1 | Mouse | monoclonal, HO-22.1 | Mouse | The Jackson Laboratory | TJL51 | 20-500 | ||||||||||||||||||
| Thy1.2 | Mouse | monoclonal, HO-13.4 | Mouse | The Jackson Laboratory | TJL 50 | 20-500 | ||||||||||||||||||
| Trichohyalin | Human | monoclonal, AE15 | Mouse | Dr. T.-T. Sun | Citrate (5 cycles) | 5-500 | 20 | 20 | 10 | Outer root sheath, adrenal medulla | ||||||||||||||
| Tropomyosin 1 | Human (recombinant) | Rabbit | Chemicon | AB3228 | Citrate (5 cycles) | 100 | 100 | 100 | 100 | Smooth and striated muscle fibers | ||||||||||||||
| Tyrosin hydroxylase | Rat | monoclonal, TH-16 | Mouse | Sigma | T2928 | 105H4830 | NO | 500-10,000 | 10000 | 10000 | Adrenal medulla | |||||||||||||
| Uvomorulin | Mouse | monoclonal, DECMA-1 | Rat | Sigma | U3254 | 50-2,000 | ||||||||||||||||||
| VEGF (A-20), N-terminus | Human | polyclonal | Rabbit | Santa Cruz | sc-152 | C2003 | 25-500 | |||||||||||||||||
| VEGF-R2 | Human (recombinant) | monoclonal, 260.4 | Mouse | Sigma | V3003 | NO | 200-1,000 | Artifactual labeling of endothelial cells in PFA-fixed tissues | ||||||||||||||||
| VEGF-R2 | Mouse (recombinant) | |||||||||||||||||||||||
| Vimentin | Human | Goat | Biomeda | V2009 | 9993 | 20-80 | Mesenchymal cells | |||||||||||||||||
| Vimentin | Human | Goat | Chemicon | AB1620 | 50-1,000 | |||||||||||||||||||
| Vimentin | Human (recombinant) | Rabbit | Chemicon | CBL46 | Citrate (5 cycles) | 500 | 1000 | 1000 | 1000 | 750 | Fibrocytes of the dermis, melanocytes of the epidermis, chondrocytes, smooth muscle fibers of large arteries, etc; this antibody also labels the outer root sheath, the duct of salivary glands, the conjunctiva, the corneal epithelium, etc | |||||||||||||
| Vitamin D receptor, intestinal | Chicken | Rat | Chemicon | MAB1360 | 22061355 | Citrate (5 cycles) | 50-500 | 50 | 100 | 50 | 50 | 100 | Intestinal epithelium | |||||||||||
| This table was last updated on 31 July 2003, by Dr. Igor Mikaelian, The Jackson Laboratory. Hyperlinks last updated on 20 Jan. 2009. | ||||||||||||||||||||||||
| See also: Mikaelian I et al., "Antibodies that label paraffin-embedded mouse tissues: a collaborative endeavor." Toxicol Pathol 2004 Mar-Apr;32(2):181-91. | ||||||||||||||||||||||||
| Disclaimer: This list is made available for information and suggested use. | ||||||||||||||||||||||||
| Every care has been taken to ensure the accuracy of the information supplied but some inaccuracies may be present. | ||||||||||||||||||||||||
| In particular, the catalogue numbers for antibodies and links to websites may have been changed by the manufacturer. | ||||||||||||||||||||||||
| We do not guarantee that the antibodies and procedures listed will provide optimal results under your or any other conditions. | ||||||||||||||||||||||||
| Other antibodies to the same antigens may label equally well or better. Differences in batches of the same antibody may | ||||||||||||||||||||||||
| account for differences in labeling. The listing of commercial products does not imply endorsement of the product or the | ||||||||||||||||||||||||
| manufacturer by The Jackson Laboratory. The websites listed or linked from this site are provided for information only. | ||||||||||||||||||||||||