List of Antibodies tested on mouse tissue samples               Fixatives and antibody dilutions tested      
Antigen Antigen Species Antibody type Antibody Species Provider Product No. Batch Antigen retrieval Dilution range tested Fekete's Zn Bouin's NBF PFA Recommended control tissue
15-lipoxygenase Human monoclonal, 061402DM Rabbit Oxford Biomedical Research LX25 NO 50-8,000 50 Hair follicles Legend  
a-gp27 Gag Viral (MMTV) monoclonal, Blue-7-1 Mouse The Jackson Laboratory, Dr. Tatyana Golovkina Citrate (5 cycles) 1-10 5 MMTV-infected mammary gland   Staining too pale
a-gp36 Env Viral (MMTV) monoclonal, Blue 6-1 Mouse The Jackson Laboratory, Dr. Tatyana Golovkina NO 1-10 10 MMTV-infected mammary gland   Background too high
a-gp52 Env Viral (MMTV) monoclonal, Black 6-5D Mouse The Jackson Laboratory, Dr. Tatyana Golovkina NO 1-10 5 MMTV-infected mammary gland   No signal
Alpha actinin Chicken Rabbit Sigma A2543 78F-4865 100-2,000             Good signal
Alpha catenin Human (recombinant) Rabbit Chemicon AB19021 Citrate (5 cycles) 250-1,000 250         Intestinal epithelium   Not tested
Alpha catenin Human/Mouse (synthetic peptide) Rabbit Sigma C2081 Citrate (5 cycles) 250-5,000 250 250     250 Intestinal epithelium
Alpha SHP-1 monoclonal, D-11 Rabbit Santa Cruz sc-7289 10-50          
Alpha smooth muscle actin Human (synthetic peptide) monoclonal, 1A4 Mouse Sigma A2547 NO 6,000-40,000 15000 15000 15000 15000 15000 Smooth muscle fibers, myoepithelial cells
Alpha-fetoprotein Human Rabbit Dako A0008 097 1,000-5,000          
Alpha-Sarcomeric Actin Rabbit monoclonal, 5C5 Mouse Sigma A2172 NO 5,000-20,000           Myocardium and skeletal muscles; least background with Zinc at 1:10,000
Androgen receptor (AR; N20) Human (recombinant) Rabbit Santa Cruz sc-816 A2003 Citrate (5 cycles) 100 200 100 200 200 Leydig cells (testis)
Angiopoietin 3 (M18) Mouse (recombinant) Goat Santa Cruz sc-9355  
Angiopoietin 3 (R19) Mouse (recombinant) Goat Santa Cruz sc-9357  
ARF (H50) Human (recombinant) Rabbit Santa Cruz sc-9063 B221 10-500          
Bax Human (recombinant) Rabbit R&D Systems AF820  
Bcl2 Human (recombinant) monoclonal, C-2 Mouse Santa Cruz sc-492 C1403 25-450          
bcl-2 Mouse (recombinant) Goat R&D Systems AF810  
Bcl-6 (N3) Human (recombinant) Rabbit Santa Cruz sc-858 H201 EDTA (Catoretti) 25-500 25 25       Lymphoid follicles
Beta catenin Human (recombinant) monoclonal, NA Mouse Chemicon MAB2081 Citrate (5 cycles) 100-2,000           Colon
Beta catenin Human (recombinant) Rabbit Chemicon AB19022 Citrate (5 cycles) 250-1,000 500 250 500 500   Intestinal epithelium
Beta-catenin Human/Mouse (synthetic peptide) Rabbit Sigma C2206 Citrate (5 cycles) 250-5,000 250   250 250   Colonic epithelium
Beta-catenin (C18) Human (recombinant) Goat Santa Cruz sc-1496 B1803 NO 450 150 300 150 150 Colon, duodenum, cornea, esophagus, epidermis
Bgp1 Mouse Rabbit Dr. Beauchemin Variable 2,000-10,000 10000 5,000 -10,000 5,000 5,000 -10,000 5,000 -10,000 Glands with ducts and digestive tract
Bgp2 Mouse Rabbit Dr. Beauchemin Citrate (5 cycles) 2,000-10,000 2000   2000   2000 Colon
BrdU monoclonal, Bu20a Mouse Dako M0744 033 (Aug.96) Trypsin/HCl/Sodium Borate 20 20 Epidermis, duodenum
BrdU, conjugated to KLH monoclonal, BU-33 Rat Sigma B2531 Trypsin/HCl/Sodium Borate 40 40 Epidermis, duodenum
BVP-1 Bovine papilloma virus type I Rabbit Dako B0580 NO 2000 2000 Viral papillomas
Caspase 3 Human (recombinant) Rabbit R&D Systems AF835  
Caspase 3 (CPP32) Human (recombinant) monoclonal, 4-1-18 Mouse Chemicon MAB4703 23021221 25-750   25       Small intestine
Cathepsin D Human monoclonal, C5 Mouse Chemicon MAB422 22120520 100-400  
CC26 (Clara cell protein 26 kD) Rat polyclonal Rabbit Chemicon AB3700 23030909 Variable 1600 (citrate) 800 (citrate) 1600 800 1600 Lung (also labels a variety of other tissues)
CCR3 Mouse (synthetic peptide) Rabbit BD Biosciences 556882 200-1,000          
CD106 Mouse monoclonal, 429 Rat (IgG2b) The Jackson Laboratory (Ted Duffy) TJL186B 10-50          
CD106 (VCAM-1) Mouse monoclonal, M/K-2 Rat Chemicon CBL1300 176U0266 25-500          
CD146 Human monoclonal, P1H12 Mouse Chemicon MAB16985 21121094 100-1,000          
CD19 Mouse monoclonal, ID3 Rat (IgG2b) The Jackson Laboratory (Ted Duffy) TJL167B 10-50          
CD22 Mouse monoclonal, 2D6 Rat Chemicon CBL1311 1311451723 50 50 50 50 50   Spleen, lymph nodes
CD28 (M-20) Mouse (recombinant) Goat Santa Cruz sc-1624 K198 25-500          
CD29 Mouse monoclonal, OXM718 Hamster Chemicon CBL1333 50-1000          
CD3 Mouse monoclonal, 17A2 Rat Chemicon CBL1302 13023893 100-1000          
CD3 (PC3/188A) Human monoclonal, PC3/188A Mouse Santa Cruz sc-20047 I1102 25-1000          
CD31 (PECAM-1) Mouse monoclonal, MEC13.3 Rat BD Biosciences 550274 10-100          
CD38 Mouse monoclonal, 90 Rat Chemicon CBL1338 1338516143 NO 50-500   50       B cells (spleen and Peyer's patches), thymic medulla
CD3-d (M-20) Mouse (recombinant) Goat Santa Cruz sc-1129 F010 EDTA (Catoretti) 25 25       Paracortical areas in lymph nodes, Periarteriolar lymphoid sheaths in spleen, thymus (scattered cells)
CD3e Mouse Rat The Jackson Laboratory TJL 154 P 20-500          
CD3e Mouse monoclonal, 145-2C11 Hamster The Jackson Laboratory (Ted Duffy) TJL010B 10-50          
CD3-e Mouse monoclonal, KT3 Rat Chemicon CBL1317 21080415 100-1000          
CD3-gamma (C-18) Mouse (recombinant) Goat Santa Cruz sc-1125 F010 NO 25         Intra-epithelial T cells of duodenum
CD4 Unknown monoclonal, GK1.5 Rat Chemicon CBL1305 130561P 20-500          
CD4 Mouse monoclonal, YTS191.1 Rat The Jackson Laboratory TJL 099P 100-500          
CD4 (C-18) Human (recombinant) Goat Santa Cruz sc-1140 J3002 10-200          
CD40 (C-20) Human (recombinant) Goat Santa Cruz sc-1731 J3102 10-500          
CD45 Mouse monoclonal, C363.16A Rat Chemicon CBL1343 1343227113 NO 50-500 250   250 250 50 Intraepithelial T cells in intestine, thymocytes in thymic medulla, periarteriolar lymphoid sheaths; also works on whole gastro-intestinal mounts
CD45R/B220 Mouse monoclonal, RA3-6B2 Rat Chemicon CBL1342 22010573 Variable 25-500 50 (no AgR) 125 (with AgR), 50 (w/o AgR) 100 (AgR) 100 (AgR) 100 (AgR) Lymphoid tissues
CD49d Mouse monoclonal, R1-2 Rat BD Biosciences 553154 100-2,000          
CD62L Mouse monoclonal, MEL-14 Rat Chemicon MAB2151Z 23030949 Citrate (5 cycles) 25 25 25 25    
CD68 (H-255) Human (recombinant) Rabbit Santa Cruz sc-7084 G101 Citrate (5 cycles) 400 400 400 200 200 Small intestine (scattered epithelial cells)
CD83 (FL-205) Human (recombinant) Rabbit Santa Cruz sc-20083 E132 10-200          
CD8a Mouse monoclonal, YTS169.4 Rat The Jackson Laboratory TJL O98P 20-500          
CD8a Mouse monoclonal, 53-6.72 Rat The Jackson Laboratory TJL 032 P 20-500          
cdk1/p34cdc2 Xenopus monoclonal, A17.1.1 Mouse Chemicon MAB8878 18100662 Citrate (5 cycles) 50-500          
CDK2 Human Mouse BD Biosciences 610145 1,000-10,000          
CDK4 Human (recombinant) monoclonal, DCS-35 Mouse Chemicon MAB8879 22110362 Citrate (5 cycles) 100-500          
Cdk4 (C-22) Mouse (recombinant) Rabbit Santa Cruz sc-260 A1403 10-300          
C-myc (9E10) Human (recombinant) Rabbit Santa Cruz sc-262 B2603 10-300          
Collagen IV Mouse Rabbit Chemicon AB756P Variable 20-100   100       Kidney
Collagen IV Human monoclonal, IV-4H12 Mouse Chemicon MAB3326 50-4500          
Collagen IV Mouse Rabbit Chemicon AB756 YES 500-2,500 500 500 500     Basement membranes
Cyclin A Human (recombinant) Rabbit Rockland 100-400-151 2626 100-2,000          
Cyclin A (H-432) Human (recombinant) Rabbit Santa Cruz sc-751 L1602 EDTA (Catoretti) 50 50 25 25 25 Thymus
Cyclin B1 Human (recombinant) Rabbit Rockland 100-401-152 2627 100-2,000          
Cyclin D1 Human (recombinant) Rabbit Rockland 100-401-153 11756 100-2,000          
Cyclin D1 (72-13G) Mouse (recombinant) monoclonal, 72-13G Mouse Santa Cruz sc-450 G252 EDTA (Catoretti) 100 Tumors (no labeling in non-neoplastic tissues)
Cyclin D3 (C-16) Human (recombinant) Rabbit Santa Cruz sc-182 C1803 EDTA (Catoretti) 25-100   100     100 Thymus
Cyclin E Human (recombinant) Rabbit Upstate 06-459 100-2,000          
Cytokeratin 15 Mouse monoclonal, LHK15 Mouse Chemicon CBL272 1508U0015 5 5 5 5 5 Haired skin
Desmin Human monoclonal, DE-B-5 Mouse Chemicon IHC2011-6 22090574 Citrate (5 cycles) 1 1 1 1 1 Smooth muscles and striated skeletal muscles; also artifactually labels the transitional epithelium, the cornea, salivary glands, and mammary luminal cells with some fixatives
DVN-1 Mouse Mouse Dr. Derry Roopenian NO           Labeling with many fixatives (1:100) and in frozen tissues in wt and transgenic mouse
EGF Mouse Rabbit Sigma E2635 NO 1,000-5,000 2000   1000   2000 Ducts of salivary glands, epidermis
EGFR Human (synthetic peptide) monoclonal Mouse Sigma E3138 Citrate (5 cycles) 500-5,000 500 500       Axones of peripheral nerve
Eosinophil peroxidase Human monoclonal Mouse Chemicon MAB1087 21080539 Citrate (5 cycles) 50-500 150 skin of cpdm mice
Eosinophilic major basic protein Unknown monoclonal, AHE-2 Mouse Chemicon MAB1086 21120015 50-500  
Eosinophilic major basic protein Human monoclonal, BMK13 Mouse Chemicon CBL419 4192705P 50-500  
Ephrin B1 (C-18) Human recombinant (identical to mouse ephrin B1 and B2) Rabbit Santa Cruz sc-910 A0803 Citrate (5 cycles) 100 100 50 200 100 Colon and duodenum (scattered epithelial cells)
ERB-4 (C-18) Human (recombinant) Rabbit Santa Cruz sc-283 C0603 Citrate (5 cycles) 800 600 600 600   Nuclear labeling of the cells of the crypts of small intestine, cardiomyocytes, neurones
erbB2 Rat monoclonal, 42 Mouse BD Biosciences 610161 1,000-5,000          
Estrogen receptor (aa120-170) Bovine monoclonal, AER304 Mouse Chemicon MAB443 50-500          
F4/80 (macrophages) Mouse Rat The Jackson Laboratory TJL O46P 50-500          
Factor VIII-related antigen Human Mouse Dako M0616 093 50-1000          
Fas/TNFRSF6 (CD95) Mouse (recombinant) Goat R&D Systems AF435  
Fibronectin Human monoclonal, FN-15 Mouse Sigma F7387 100-2,000          
Filaggrin Mouse Rabbit Covance PRB-417P NO 5,000-35,000 15000 10000 10000 5000 5000 Stratum corneum
Flk1 Mouse (recombinant) monoclonal, A3 Mouse Santa Cruz sc-6251 100-2,000          
Gamma catenin Human (recombinant) monoclonal, 11 E4 Mouse Chemicon MAB2083 Citrate (5 cycles) 100-2,000          
Glial fibrillary acidic protein (GFAP) Human Rabbit Sigma G9269 Citrate (5 cycles) 100-7,500 7500   2500 Glial cells
Ha1 Human Mouse Dr. Fleckman 1-50          
Ha2 Human Mouse Dr. Fleckman 1-50          
Ha5 Human Mouse Dr. Fleckman 1-50          
Hb1 Human Mouse Dr. Fleckman 1-50          
IgG4 Human monoclonal, G17-4 Mouse BD Biosciences 555882 30238 50-130          
IL7R-alpha Mouse monoclonal, A7R34 Rat eBioscience 14-1271 100   100 100   Granulocytes
Integrin alpha 6 Unknown monoclonal, MA6 Rat Chemicon MAB1982 25-1,000          
Integrin beta 1 Unknown monoclonal, MB1.2 Rat Chemicon MAB1997 Citrate (5 cycles) 50-1,000   1000       Intestine
Involucrin Mouse Rabbit Covance PRB-142C NO 4,000-10,000 7500   7500 7500 Epidermis
IRF-4 (M-17) Mouse (recombinant) Goat Santa Cruz sc-6059 J0802 EDTA (Catoretti) 25 25 50
JAK1 Human/Mouse (recombinant) Rabbit Upstate 06-272 Citrate (5 cycles) 100-2,000 100   100 500   Gastric epithelium epidermis
JAK2 Mouse (recombinant) Rabbit Upstate 06-255 Citrate (5 cycles) 100-2,000 100       100 Urothelium., most epithelia
Keratin 1 Mouse Rabbit Covance PRB-165P NO 6,000-25,000 12000   6000   12000 Epidermis
Keratin 1 Human Mouse Dr. Fleckman 1-25           Haired skin
Keratin 10 Mouse Rabbit Covance PRB-159P NO 2,500-15,000 2500 2500 2500   2500 Epidermis
Keratin 10 Human monoclonal, DE-K10 Mouse Dr. Fleckman, NeoMarkers MS-611 Citrate (5 cycles) 50-1,000 200   50   50 Haired skin
Keratin 13 Human monoclonal, Ks 13.1 Mouse Chemicon CBL176 NO 100-500 100 200 100   100 Epidermis
Keratin 13 Human monoclonal, KS-1A3 Mouse Sigma C0791 50-1,000          
Keratin 14 Mouse Rabbit Covance PRB-155P NO 6,000-25,000 25000 12000 12000 25000 6000 Epidermis
Keratin 14, C-terminus Human (recombinant) monoclonal, LL002 Mouse Dr. Fleckman, NeoMarkers MS-115 Citrate (5 cycles) 50-500 50 100 50   50 Oral mucosa, haired skin
Keratin 15, C-terminus Human (recombinant) monoclonal, LHK15 Mouse Dr. Fleckman MS-1068 Citrate (5 cycles) 5-500 5 5     5 Haired skin
Keratin 16, C-terminus Human (recombinant) monoclonal, LL025 Mouse Dr. Fleckman MS-620 Digestion 1-500 1       Oral mucosa, haired skin
Keratin 17 Rat monoclonal Mouse Chemicon MAB1677 1-200          
Keratin 17 Rat monoclonal, E3 Mouse Dr. Fleckman, NeoMarkers MS-489 50-1,000           Cornea, epidermis, mammary gland
Keratin 17 Mouse (recombinant) Rabbit Dr. Pierre Coulombe NO 750-2,500 2,500 750 2,500 2,500 2,500 Hair follicles; also labels the wall of some arteries
Keratin 18 Human monoclonal, Ks18.04 Mouse Chemicon CBL177 100-2,000          
Keratin 18 Human monoclonal, DC10 Mouse Chemicon MAB1600 100-2,000          
Keratin 18 Unknown monoclonal, RGE53 Mouse Chemicon MAB3234 Digestion 100-2,000 500   200   500 Ducts and ductules in exocrine pancreas and salivary glands
Keratin 18 Human monoclonal, M9 Mouse Monosan MON 3006 10-50          
Keratin 20 Human monoclonal, Ks 20.8 Mouse Chemicon CBL208 50-1,000          
Keratin 3/12 Rabbit monoclonal, AE5 Mouse Chemicon CBL218 21812101p-01 Digestion 50-500 200   200 200 200 Haired skin
Keratin 5 Mouse Rabbit Covance PRB-160P NO 7,000-30,000 7000   7000   7000 Epidermis
Keratin 6 Mouse Rabbit Covance PRB-169P NO 6,000-25,000 12000 6000 6000   6000 Epidermis
Keratin 6, C-terminus Human (recombinant) monoclonal, LHK6B Mouse Dr. Fleckman, NeoMarkers MS-766 Citrate (5 cycles) 5-500           Haired skin
Keratin 6a Guinea pig Dr. Roop K6-212 Citrate (5 cycles) 500-2,000 500   500     Oral mucosa, haired skin
Keratin 6a/b Rabbit Dr. Roop K6-83 Citrate (5 cycles) 500-2,000 500 500 1000 500 500 Haired skin
Keratin 6b Rabbit Dr. Roop K6-82 Citrate (5 cycles) 250-2,000           Oral mucosa, haired skin
Keratin 7 Human monoclonal, LP5K Mouse Chemicon CBL194 NO 25 25   Urothelium
Keratin 7 Human monoclonal, LdS68 Mouse Chemicon CBL184 50-1,000          
Keratin 7 Unknown monoclonal, RCK105 Mouse Monosan MON 3007 Digestion 25-1,000 50         Outer root sheath; does not label the structures it is supposed to label (transitional epithelium)
Keratin 8 Human monoclonal, LP3K Mouse Chemicon CBL195 100-500          
Keratin 8 Human monoclonal, NA Mouse Chemicon MAB1634 100-2,000          
Keratin 8 Human monoclonal, LP3K Mouse Chemicon CBL195 100-2,000          
Keratin 8 monoclonal, EL41 Potoroo Dr. E.B. Lane 5          
Keratin 8, 18 Bovine Guinea pig Progen GP11 Citrate (5 cycles) 300-600 450 450 600     Colonic epithelium; also labels structures it is not supposed to label
Keratin 8/18 monoclonal, EL61 Potoroo Dr. E.B. Lane 5          
Keratins type I Unknown monoclonal, AE1 Mouse Chemicon CBL215 Variable 100-500 200 200 150 (AgR)     Haired skin
Keratins type II Human monoclonal, AE3 Mouse Chemicon MAB1611 NO 50-1,000 50 50 50     Haired skin; does not labell all structures it is supposed to label
Keratins 4, 5, 6, 8, 10, 13, 18 Human monoclonal, C11 Mouse Chemicon CBL234 Digestion 50-1,000 50         Does not label all structures it is supposed to label
Keratins 4, 5, 6, 8, 10, 13, 18 Unknown monoclonal Mouse Chemicon MAB1636 NO 50-1,200 1000 1000 1000     Haired skin
Keratins 5, 14 Human monoclonal, LH8 Mouse Chemicon CBL267 25-1,000          
Keratins 5, 6 Human monoclonal, D5/16 B4 Mouse Chemicon MAB1620 Citrate (5 cycles) 50-200 100   50     Haired skin
Keratins 5, 8 Human monoclonal Mouse Chemicon MAB3228 NO 50-1,000 250 250 250 250   PFA is great for the CL of the ORS; discrepencies between the fixatives
Keratins 5, 8 Unknown monoclonal Mouse Chemicon MAB1629 Digestion 50-1,000 500 500 500   500 Esophagus, tongue; does not label all structures it is supposed to label
Keratins 7, 17 Unknown monoclonal Mouse Chemicon MAB1625 50-1,750           Haired skin, oral mucosa
Keratins 8, 18 Human monoclonal, NCL-5D3 Mouse Chemicon CBL170 Digestion 25-500 25   25     Colonic epithelium
Ki67 (MKI67) Human monoclonal, B56 Mouse BD Biosciences 550609 Citrate (5 cycles) 25-100 25 25       Intestinal epithelium, lymphoid follicles
Ki-67 (MKI67) Human (recombinant) monoclonal Mouse Invitrogen 08-0156  
Ki67 (Mki67, M-19) Mouse (recombinant) Goat Santa Cruz sc-7846 A3103 Citrate (5 cycles) 200 200 200 200 200 Thymus
Laminin Mouse Rabbit Sigma L9393 NO 3,000-15,000 3000 3000       Basement membrane of skeletal muscle fibers and blood vessels
Loricrin Mouse Rabbit Covance PRB-145P NO 4,000-15,000 8000 8000 8000 8000   Epidermis
L-selectin (CD62L) Mouse monoclonal, MEL-14 Rat Chemicon MAB2151Z 21031198 25-750          
M30 CytoDEATH (cytokeratin 18) Human monoclonal, M30 Mouse Roche 2 140 322  
Mac-1 (CD11b) Mouse monoclonal Rat (IgG2b) The Jackson Laboratory, Ted Duffy TJL063B 10-50          
Major Basic Protein Mouse Rabbit Dr. Elizabeth Lenkiewicz Rabbit 509 (10/16/98) Digestion 1.5 min 2000-4000 2000 cpdm mouse tissues
MHC Class II (Ia) Mouse monoclonal, M5/114 Rat The Jackson Laboratory, Ted Duffy 250 250 250 250 250 250
NCAM (CD56) Chicken Rabbit Chemicon AB5032 23010388 650 650 650 650 650
NCAM (CD56) Mouse Rat Chemicon MAB310 23020285 Citrate (5 cycles) 100 200 50 50 50
Neurofilament Bovine Rabbit Sigma N4142 NO 3,500-15,000 7000         Axones of peripheral nerves, neurones
Neuron-specific enolase (NSE) Bovine Rabbit Dako A0587 NO 4,000-15,000 15000 8000     Peripheral nerves, ganglion cells
NK Mouse monoclonal, Dx5 Rat The Jackson Laboratory TJL 148 20-500          
NK1.1 cells Mouse monoclonal, PK136 Mouse The Jackson Laboratory TJL 83 20-500          
NLDC 145 (dendritic cells) Mouse Rat The Jackson Laboratory TJL 122 20-500          
NOS2 Human (recombinant) Rabbit Santa Cruz sc-651 750 1000 1000 1000 1250 Dendritic cells in thymus (nuclear)
ODC Mouse (recombinant) monoclonal, ODC-29 Mouse Sigma O1136 3,500-15,000           Corneal epithelium shows mild diffuse labelling whenever available: artifact? Possible artifactual labeling of epithelial cells of the colon, brush border of proximal tubules of the kidney, etc at a dilution of 1:750 with AgR (Bouin's and Fekete's)
p15 (M-20) Mouse (recombinant) Goat Santa Cruz sc-1429 D101 Variable 100     100 100 (citrate buffer) Bone marrow
p16 (H-156) Human (recombinant) Rabbit Santa Cruz sc-1207 K281 50-200          
p19 (M-167) Mouse (recombinant) Rabbit Santa Cruz sc-1063 E170 50-200          
p21 Mouse (recombinant) monoclonal, F-5 Mouse Santa Cruz sc-6246 B1003 100 Mammary tumors in mice transgenic for myc and wnt1
p27/Kip1 (C19) Human recombinant) monoclonal, C-19 Rabbit Santa Cruz sc-528 C1303 Citrate (5 cycles) 25 25 25 25 25 Small intestine; Bouin's is the best but labeling is only moderate and there is background
p53 Human (recombinant) monoclonal, DO-7 Mouse BD Biosciences 550831 Citrate (5 cycles) 25-100 25 Some tumors
p63 Human (recombinant) monoclonal, 4A4 Mouse Chemicon MAB4135 2212133 NO 250-750 750 750 750 750 750 Transtional epithelium, male accessory sexual glands
p63 Human recombinant monoclonal, 4A4 Mouse Santa Cruz sc-8431 L0302 EDTA (Catoretti) 25 25 10 25 50 Basal layers of esophagus, epidermis, mammary gland, prostate, vagina, etc
Pancadherin Human (synthetic peptide) Rabbit Sigma C3678 NO 1,000-100,000 5000 5000 5000 5000 5000 Epidermis, colon, placenta
Pax Mouse (recombinant) Rabbit Covance PRB-278P 500-1,000          
PCNA Mouse (recombinant) monoclonal, PC10 Mouse Santa Cruz sc-56 A0903 Variable 500 500 (citrate) 750 100 500 Duodenum, thymus
PECAM 1 (M-20) Mouse (recombinant) Goat Santa Cruz sc-1506 25-500           Works on whole mounts (endothelial cells) at 1:400
PECAM-1 Mouse monoclonal, 390 Rat Chemicon CBL1337 133715882P 25-750          
Phospho-beta-catenin (Ser33/37Thr41) Human (synthetic) Rabbit Cell Signaling 9561 NONE          
Phospho-beta-catenin (Thr41/Ser45) Human (synthetic) Rabbit Cell Signaling 9565 NONE          
P-selectin Mouse Rabbit Chemicon CBL40 40161P-01 EDTA (Catoretti) 50 50 50 50 25 Spleen and bone marrow (megakaryocytes, platelets)
Rb (C-15) Human (recombinant) Rabbit Santa Cruz sc-50 B0503 25-1000          
Rel B Mouse (recombinant) Rabbit Santa Cruz sc-226 C2803 500 500 1000 1000 1000 Nucleus of some dendritic cells and M cells of Peyer's patches; slightly better with citrate buffer antigen retrieval
S100 Bovine monoclonal, 15E2E2 Rabbit Chemicon MAB079 22011259 Citrate (5 cycles) 1,000-20,000           Axones of peripheral nerves, cartilage, neurones
S100 Bovine Mouse Sigma S2644 100-500          
Shh, C-terminus Mouse (recombinant) Goat R&D Systems AF445  
Shh, N-terminus Mouse (recombinant) Goat R&D Systems AF464  
TGF-beta Human (recombinant) Rabbit Sigma T9429 NO 500-10,000 1000     1000 Epidermis
Thy1.1 Mouse monoclonal, HO-22.1 Mouse The Jackson Laboratory TJL51 20-500          
Thy1.2 Mouse monoclonal, HO-13.4 Mouse The Jackson Laboratory TJL 50 20-500          
Trichohyalin Human monoclonal, AE15 Mouse Dr. T.-T. Sun Citrate (5 cycles) 5-500 20 20     10 Outer root sheath, adrenal medulla
Tropomyosin 1 Human (recombinant) Rabbit Chemicon AB3228 Citrate (5 cycles) 100 100 100 100     Smooth and striated muscle fibers
Tyrosin hydroxylase Rat monoclonal, TH-16 Mouse Sigma T2928 105H4830 NO 500-10,000 10000 10000 Adrenal medulla
Uvomorulin Mouse monoclonal, DECMA-1 Rat Sigma U3254 50-2,000          
VEGF (A-20), N-terminus Human polyclonal Rabbit Santa Cruz sc-152 C2003 25-500          
VEGF-R2 Human (recombinant) monoclonal, 260.4 Mouse Sigma V3003 NO 200-1,000           Artifactual labeling of endothelial cells in PFA-fixed tissues
VEGF-R2 Mouse (recombinant)          
Vimentin Human Goat Biomeda V2009 9993 20-80           Mesenchymal cells
Vimentin Human Goat Chemicon AB1620 50-1,000          
Vimentin Human (recombinant) Rabbit Chemicon CBL46 Citrate (5 cycles) 500 1000 1000 1000 750 Fibrocytes of the dermis, melanocytes of the epidermis, chondrocytes, smooth muscle fibers of large arteries, etc; this antibody also labels the outer root sheath, the duct of salivary glands, the conjunctiva, the corneal epithelium, etc
Vitamin D receptor, intestinal Chicken Rat Chemicon MAB1360 22061355 Citrate (5 cycles) 50-500 50 100 50 50 100 Intestinal epithelium
This table was last updated on 31 July 2003, by Dr. Igor Mikaelian, The Jackson Laboratory. Hyperlinks last updated on 20 Jan. 2009.
See also: Mikaelian I et al., "Antibodies that label paraffin-embedded mouse tissues: a collaborative endeavor." Toxicol Pathol 2004 Mar-Apr;32(2):181-91.
Disclaimer: This list is made available for information and suggested use.
Every care has been taken to ensure the accuracy of the information supplied but some inaccuracies may be present.
In particular, the catalogue numbers for antibodies and links to websites may have been changed by the manufacturer.
We do not guarantee that the antibodies and procedures listed will provide optimal results under your or any other conditions.
Other antibodies to the same antigens may label equally well or better. Differences in batches of the same antibody may
account for differences in labeling. The listing of commercial products does not imply endorsement of the product or the
manufacturer by The Jackson Laboratory. The websites listed or linked from this site are provided for information only.