PDX Model DetailsJ000080739

Request information on the availability of this model.

PDX Model Summary

Model Details

Model ID
J000080739
Initial Diagnosis
Invasive ductal carcinoma
Final Diagnosis
Invasive ductal carcinoma
Stage/Grade
AJCC IV / Grade:3
Primary Site
Breast
Sample Site
Breast
Tumor Type
Primary Malignancy

Publications citing this model

Malcolm, J; et al. Factors that influence response classifications in chemotherapy treated patient-derived xenografts (PDX). PeerJ. 2019 Mar 28;7:e6586.  10.7717/peerj.6586

Patient

Sex
Female
Age (at time of tissue collection)
49
Race/Ethnicity
White / Not Reported
Treatment Naïve?
No
Current Smoker/Former Smoker
Unknown / No

Engraftment

Host Strain
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
(aka NSG or NOD Scid gamma)
Sample Type
Surgical resection (fragment)
Engraftment Site
Right Flank

Molecular Data (Engrafted Tumor)

Variants

Gene Expression

Platforms: RNA_Seq

Hatched bars indicate genes with expression levels determined to be mildly affected by alignment calculations. Details.

Gene CNV

CNV Plots

Tumor Mutation Burden

Sample J000094325 from passage P0 has a TMB score of 8.08
TMB Range
0.0-304.42 (across all JAX PDX models; TMB > 22 is considered high)

Microsatellite Instability

Sample:J000094325 from passage P0     Score:MSI-S

Gene Fusion

no data

Model Characterization

Histology

Summary:
Moderate concordance of morphologic features between patient tumor and P0 tumor with sheets of high grade carcinoma. P0, P1, P3 pathology consistent with breast carcinoma. P2 pathology consistent with carcinoma.
Pathologist:
Dr. Regina Gandour-Edwards, University of California, Davis

Patient tumor for PDX model J000080739. Undifferentiated tumor with 25% necrosis.

Patient tumor (PT) for PDX model J000080739 stained for H&E, shows sheets of high grade carcinoma.

Patient tumor (PT) for PDX model J000080739 immunostained for ER. Staining was negative.

Patient tumor for PDX model J000080739 immunostained for ERBB2 (HER2). Staining was negative.

Patient tumor for PDX model J000080739 immunostained for PR. Staining was negative.

P0 tumor #5 for PDX model J000080739.

P0 tumor for PDX model J000080739 stained for H&E, shows Sheets of high grade carcinoma.

P0 tumor for PDX model J000080739 immunostained for ER. Staining was negative.

P0 for PDX model J000080739 immunostained for ERBB2 (HER2). Staining was negative.

P0 tumor for PDX model J000080739 immunostained for PR. Staining was negative.

P0 tumor for PDX model J000080739 immunostained for vimentin. Staining was positive in infiltrating inflammatory cells (likely lymphocytes).

P0 tumor for PDX model J000080739 immunostained for Ki67. Staining was positive.

P1 tumor for PDX model J000080739 stained for H&E, shows sheets of high grade carcinoma.

P1 tumor for PDX model J000080739 immunostained for ER. Staining was negative.

P1 tumor for PDX model J000080739 immunostained for ERBB2 (HER2). Staining was negative.

P1 tumor for PDX model J000080739 immunostained for PR. Staining was negative.

P1 tumor for PDX model J000080739 immunostained for Vimentin. Vimentin staining Positive in scattered tumor cells at the edges of tumor sheets. Possible early EMT.

P1 tumor for PDX model J000080739 immunostained for Ki67. Staining was positive.

P2 tumor F005 ID012 for PDX model J000080739 stained for H&E.

P2 tumor F005 ID012 for PDX model J000080739 stained for AE1/AE3. Staining was positive.

P2 tumor F005 ID012 for PDX model J000080739 stained for AR. Staining was positive.

P2 tumor F005 ID012 for PDX model J000080739 stained for CD45. Staining was negative.

P2 tumor F005 ID012 for PDX model J000080739 stained for ER. Staining was negative.

P2 tumor F005 ID012 for PDX model J000080739 stained for ERBB2 (HER2). Staining was negative.

P2 tumor F005 ID012 for PDX model J000080739 stained for Ki67. Staining was positive.

P2 tumor F005 ID012 for PDX model J000080739 stained for PR. Staining was negative.

P3 tumor F005 ID020 for PDX model J000080739 immunostained for AR. Staining was negative.

P3 tumor F005 ID020 for PDX model J000080739 immunostained for ER. Staining was negative.

P3 tumor F005 ID020 for PDX model J000080739 immunostained for ERBB2 (HER2). Staining was negative.

P3 tumor F005 ID020 for PDX model J000080739 immunostained for PR. Staining was negative.

Dosing Studies

Circos Plots

no data