Reference SummaryHuang S, Genome Biol 2005;6(10):R84

Title

Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice.

Authors

Huang S; Li Y; Chen Y; Podsypanina K; Chamorro M; Olshen AB; Desai KV; Tann A; Petersen D; Green JE; Varmus HE

Journal

Genome Biol

Volume

6

Issue

10

Year

2005

Pages

R84

Abstract

BACKGROUND: In human breast cancer normal mammary cells typically develop into hyperplasia, ductal carcinoma in situ, invasive cancer, and metastasis. The changes in gene expression associated with this stepwise progression are unclear. Mice transgenic for mouse mammary tumor virus (MMTV)-Wnt-1 exhibit discrete steps of mammary tumorigenesis, including hyperplasia, invasive ductal carcinoma, and distant metastasis. These mice might therefore be useful models for discovering changes in gene expression during cancer development. RESULTS: We used cDNA microarrays to determine the expression profiles of five normal mammary glands, seven hyperplastic mammary glands and 23 mammary tumors from MMTV-Wnt-1 transgenic mice, and 12 mammary tumors from MMTV-Neu transgenic mice. Adipose tissues were used to control for fat cells in the vicinity of the mammary glands. In these analyses, we found that the progression of normal virgin mammary glands to hyperplastic tissues and to mammary tumors is accompanied by differences in the expression of several hundred genes at each step. Some of these differences appear to be unique to the effects of Wnt signaling; others seem to be common to tumors induced by both Neu and Wnt-1 oncogenes. CONCLUSION: We described gene-expression patterns associated with breast-cancer development in mice, and identified genes that may be significant targets for oncogenic events. The expression data developed provide a resource for illuminating the molecular mechanisms involved in breast cancer development, especially through the identification of genes that are critical in cancer initiation and progression.

Links

16207355 – National Library of Medicine/PubMed

Strain Notes

Strain Note
FVB/N-Tg(MMTVneu)202Mul/J These mice were purchased from The Jackson Laboratory.
STOCK Tg(Wnt1)1Hev The genetic background of these mice was stated to be FVB (>75%), SJL, and C57BL/6.

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB Adipose tissue normal tissue (control) Adipose tissue

not applicable

STOCK Tg(Wnt1)1Hev Mammary gland hyperplasia Mammary gland

observed

FVB Mammary gland normal tissue (control) Mammary gland

not applicable

STOCK Tg(Wnt1)1Hev Mammary gland tumor Mammary gland

observed

FVB/N-Tg(MMTVneu)202Mul/J Mammary gland tumor Mammary gland

observed

STOCK Tg(Wnt1)1Hev Trp53tm1Brd Mammary gland tumor Mammary gland

observed

STOCK Ptentm1Rps/+ Tg(Wnt1)1Hev Mammary gland tumor Mammary gland

observed