Reference SummaryChen B, Carcinogenesis 1994 Sep;15(9):2031-5

Title

Allele-specific activation and expression of the K-ras gene in hybrid mouse lung tumors induced by chemical carcinogens.

Authors

Chen B; You L; Wang Y; Stoner GD; You M

Journal

Carcinogenesis

Volume

15

Issue

9

Year

1994

Pages

2031-5

Abstract

A mouse hybrid, (C3H x A/J)F1 or C3A, was developed by crossing male A/J mice (high lung tumor susceptibility) with female C3H mice (low lung tumor susceptibility). The lung tumor responses of the C3A mice to dimethylnitrosamine (DMN) or benzo[a]pyrene (B[a]P) were found to be intermediate between those of the two parental strains. Mutational activation of the K-ras gene was found at a high frequency in both the B[a]P- and DMN-induced C3A lung tumors. To explore the genetic basis of the K-ras gene involvement in mouse lung tumor susceptibility, the parental origin of the K-ras oncogene in the chemically induced C3A lung tumors was determined. K-ras oncogenes were found on the allele inherited from the susceptible A/J parent in 14/16 of DMN-induced tumors and 15/17 of B[a]P-induced tumors from C3A mice. Furthermore, the K-ras mRNA transcribed from the A/J allele was 5-20 times more than C3H K-ras transcripts in 10/10 DMN-induced and 10/10 B[a]P-induced C3A lung tumors. These data suggest that an activated A/J K-ras allele could be more tumorigenic than an activated C3H allele due to the differential expression of the two alleles in lung cells.

Links

J:20674 – MGI References
7923598 – National Library of Medicine/PubMed

Strain Notes

Strain Note
(C3H/HeJ x A/J)F1 Expression of the A/J allele of Kras2 in normal lung tissue was found to be 2-2.5 times that of the C3H allele.

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
(C3H/HeJ x A/J)F1 Lung adenoma
  • dimethylnitrosamine (DMN)
Lung

100

(C3H/HeJ x A/J)F1 Lung adenoma
  • benzo[a]pyrene (BP) (BaP) (B[a]P)
Lung

85

(C3H/HeJ x A/J)F1 Lung adenoma Lung

0 - 11

A/J Lung adenoma
  • dimethylnitrosamine (DMN)
Lung

100

A/J Lung adenoma
  • benzo[a]pyrene (BP) (BaP) (B[a]P)
Lung

95

A/J Lung adenoma Lung

25 - 42

C3H/HeJ Lung adenoma
  • dimethylnitrosamine (DMN)
Lung

10

C3H/HeJ Lung adenoma Lung

0