Reference SummaryTennent BJ, Int J Cancer 1989 Sep 15;44(3):477-82

Title

 Epidermal growth factor receptors in spontaneous ovarian granulosa cell tumors of SWR-derived mice.

Authors

 Tennent BJ; Beamer WG; Shultz LD; Adamson ED

Journal

 Int J Cancer

Volume

 44

Issue

 3

Year

 1989

Pages

 477-82

Abstract

 Epidermal growth factor (EGF) receptor binding properties were examined in spontaneous ovarian granulosa cell (GC) tumors from SWR and SWR-derived strains of mice. EGF binding was measured at room temperature in tissue homogenates from GC tumors and normal ovaries from adult randomly cycling mice. GC tumor tissue displayed significantly increased EGF binding and 2 receptor populations (R1 and R2). Normal ovarian tissue appeared to have only one receptor population with a dissociation constant (KD) similar to the R1 (high-affinity) receptor in GC tumors. In subsequent experiments, GC tumor and normal granulosa cells from immature mice were analyzed in primary cultures for EGF binding, immunofluorescence microscopy for receptors, and cell proliferation. After 24 hr in culture, the GC tumors bound 10-fold more EGF/micrograms protein than did normal granulosa cells. GC tumor cells, but not normal granulosa cells, showed specific immunofluorescence when reacted with a polyclonal antibody to mouse EGFR. During 96 hr in culture, GC tumor cells, but not normal cells, showed a significant proliferative response to EGF. In conclusion, the EGF binding capacity is markedly increased in GC tumor cells and the proliferation data suggest that this growth factor supports tumor growth in the SWR model system.

Links

 J:48592 – MGI References
2789196 – National Library of Medicine/PubMed

Strain Notes

Strain Note
(SJL/Bm x SWR/Bm)F1 The wild type allele of the Egfr gene was reported to be expressed in the ovaries of these mice. (Protein detected by binding assays.)
SJL/J The wild type allele of the Egfr gene was reported to be expressed in the ovaries of these mice. (Protein detected by binding assays.)

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
SWXJ9/BmJ Ovary - Granulosa cell tumor Ovary - Granulosa cell

observed
(SWR/Bm x SWXJ9/Bm)F1 Ovary - Granulosa cell tumor Ovary - Granulosa cell

observed
SJL/Bm Ovary - Granulosa cell tumor Ovary - Granulosa cell

0
SJL/J Ovary - Granulosa cell tumor Ovary - Granulosa cell

0
(SJL/Bm x SWR/Bm)F1 Ovary - Granulosa cell tumor Ovary - Granulosa cell

0