Reference SummaryPapanikolaou A, Cancer Lett 1998 Aug 14;130(1-2):29-34

Title

Azoxymethane-induced colon tumors and aberrant crypt foci in mice of different genetic susceptibility.

Authors

Papanikolaou A; Wang QS; Delker DA; Rosenberg DW

Journal

Cancer Lett

Volume

130

Issue

1-2

Year

1998

Pages

29-34

Abstract

Azoxymethane (AOM) is an organotropic colon carcinogen that is commonly used to induce colon tumors in rodents. Unlike its parent compound, 1,2-dimethylhydrazine (DMH), a tumor susceptibility phenotype in inbred mice with respect to AOM has not been established. Thus, this study was undertaken to determine whether genetic susceptibility extends to this carcinogen. SWR/J, A/J (both susceptible to DMH carcinogenesis) and AKR/J (resistant) mice were treated with 10 mg/kg AOM i.p. once a week for 8 weeks. Twenty-five weeks after the initial injection, tumor yield was determined. With a single exception, only SWR/J and A/J mice developed tumors, with a distribution that was limited to the distal colon (16.3+/-1.1 and 36.4+/-2.4. respectively). The formation of aberrant crypt foci (ACF), putative preneoplastic lesions, was also assessed in whole-mount colons using Methylene Blue staining. Consistent with tumor multiplicity, the total number of ACF was highest in A/J mice, followed by SWR/J mice. In addition, A/J mice had a significantly greater number of large ACF (five or more crypts per foci) than the other strains. Despite the absence of colon tumors, however, AKR/J mice did develop a significant number of ACF. This finding suggests that ACF in resistant mice are persistent but do not progress to tumors.

Links

9751253 – National Library of Medicine/PubMed

Strain Notes

Strain Note
A/J Mice were purchased from The Jackson Laboratory.
AKR/J Mice were purchased from The Jackson Laboratory.
SWR/J Mice were purchased from The Jackson Laboratory.

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
SWR/J Intestine - Large Intestine - Colon - Distal adenocarcinoma
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

AKR/J Intestine - Large Intestine - Colon - Distal adenoma
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

13

AKR/J Intestine - Large Intestine - Colon - Distal adenoma Intestine - Large Intestine - Colon - Distal

0

A/J Intestine - Large Intestine - Colon - Distal adenoma - polypoid
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

SWR/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

SWR/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF) Intestine - Large Intestine - Colon - Distal

0

A/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF) Intestine - Large Intestine - Colon - Distal

0

A/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

AKR/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

AKR/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF) Intestine - Large Intestine - Colon - Distal

0

SWR/J Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

SWR/J Intestine - Large Intestine - Colon - Distal tumor Intestine - Large Intestine - Colon - Distal

0

A/J Intestine - Large Intestine - Colon - Distal tumor Intestine - Large Intestine - Colon - Distal

0

A/J Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

SWR/J Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

SWR/J Intestine - Large Intestine - Colon - Proximal tumor Intestine - Large Intestine - Colon - Proximal

0

A/J Intestine - Large Intestine - Colon - Proximal tumor Intestine - Large Intestine - Colon - Proximal

0

A/J Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

AKR/J Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

AKR/J Intestine - Large Intestine - Colon - Proximal tumor Intestine - Large Intestine - Colon - Proximal

0

SWR/J Intestine - Small Intestine tumor
  • azoxymethane (AOM)
Intestine - Small Intestine

0

SWR/J Intestine - Small Intestine tumor Intestine - Small Intestine

0

A/J Intestine - Small Intestine tumor Intestine - Small Intestine

0

A/J Intestine - Small Intestine tumor
  • azoxymethane (AOM)
Intestine - Small Intestine

0

AKR/J Intestine - Small Intestine tumor
  • azoxymethane (AOM)
Intestine - Small Intestine

0

AKR/J Intestine - Small Intestine tumor Intestine - Small Intestine

0

SWR/J Kidney tumor
  • azoxymethane (AOM)
Kidney

0

SWR/J Kidney tumor Kidney

0

A/J Kidney tumor Kidney

0

A/J Kidney tumor
  • azoxymethane (AOM)
Kidney

0

AKR/J Kidney tumor
  • azoxymethane (AOM)
Kidney

0

AKR/J Kidney tumor Kidney

0

SWR/J Liver tumor
  • azoxymethane (AOM)
Liver

0

SWR/J Liver tumor Liver

0

A/J Liver tumor Liver

0

A/J Liver tumor
  • azoxymethane (AOM)
Liver

0

AKR/J Liver tumor
  • azoxymethane (AOM)
Liver

0

AKR/J Liver tumor Liver

0