Reference SummaryBuzard GS, J Cancer Res Clin Oncol 1999 Dec;125(12):653-9

Title

 Activation of neu by missense point mutation in the transmembrane domain in schwannomas induced in C3H/HeNCr mice by transplacental exposure to N-nitrosoethylurea.

Authors

 Buzard GS; Enomoto T; Anderson LM; Perantoni AO; Devor DE; Rice JM

Journal

 J Cancer Res Clin Oncol

Volume

 125

Issue

 12

Year

 1999

Pages

 653-9

Abstract

 Transplacentally initiated schwannomas in mice and rats arise preferentially in the Gasserian ganglion of the trigeminal nerve and spinal root ganglia, while those of the Syrian golden hamster most commonly occur subcutaneously. Rat and hamster schwannomas almost invariably contain a mutationally activated neu oncogene. In rat schwannomas, the mutant allele predominates, while the relative abundance of mutant alleles is very low in hamster nerve tumors. We investigated whether neu is mutated in mouse schwannomas and whether the pattern and allelic ratio of the mutation resemble those for the hamster or the rat. Pregnant C3H/HeNCr mice received 0.4 micromol N-nitrosoethylurea/g body weight on day 19 of gestation. Ten trigeminal and one peripheral nerve schwannomas developed in 11 of the 201 offspring. Missense T --> A transversion mutations were detected in the neu transmembrane domain in eight of ten schwannomas analyzed, as determined by MnlI digestion of polymerase chain reaction products. The mutant allele was predominantly detected in two tumors and was abundant in six others. Transfection of eight out of ten mouse tumor DNAs into hamster cells yielded transformed foci; seven out of eight contained mutant mouse neu. Mouse schwannomas closely resembled those of rats both in the preferred anatomical site and in the mutant/wild-type neu allele ratios.

Links

 J:58827 – MGI References
10592097 – National Library of Medicine/PubMed

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C3H/HeNCr Kidney lesion
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Kidney

6
C3H/HeNCr Leukocyte lymphohematopoietic neoplasm
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Leukocyte

32
C3H/HeNCr Liver lesion
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Liver

82
C3H/HeNCr Liver lesion Liver

66
C3H/HeNCr Lung lesion
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Lung

74
C3H/HeNCr Lung lesion Lung

7
C3H/HeNCr PNS - Schwann cell schwannoma
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Nerve

5.5
C3H/HeNCr PNS - Schwann cell schwannoma Nerve

0
C3H/HeNCr Pituitary gland lesion
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Pituitary gland

8
C3H/HeNCr Stomach lesion
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Stomach

12
C3H/HeNCr Thyroid gland lesion
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Thyroid gland

7