Reference SummaryMalkinson AM, J Natl Cancer Inst 1983 May;70(5):931-6

Title

Major effect on susceptibility to urethan-induced pulmonary adenoma by a single gene in BALB/cBy mice.

Authors

Malkinson AM; Beer DS

Journal

J Natl Cancer Inst

Volume

70

Issue

5

Year

1983

Pages

931-6

Abstract

Fewer lung adenomas were induced by urethan in BALB/cBy mice than in the A/J or SWR/J mouse strains. When BALB mice were crossed with either of these more sensitive strains the response of the progeny to urethan was most easily explained by a single gene which regulates susceptibility, with the more resistant phenotype behaving as a dominant trait. C56BL/6J mice were more resistant to adenoma induction than were BALB mice; progeny obtained when these two strains were crossed resembled the BALB susceptibility phenotype. As an approach to understanding the mechanism of action of this gene, agents that modulate adenoma initiation and tumor promotion were tested in BALB mice and other strains. The number of adenomas in BALB mice were increased severalfold by multiple urethan injections, which presumably affect initiation, and by the use of butylated o-hydroxytoulene as a promoting agent. Tumor incidence in A-mice was increased 50% by each treatment; neither procedure caused tumors to appear in the resistant DBA/2J, C3H/-21BG, or C57BL/6J strains. No relationship was observed between the strain dependency of the lethal effects of multiple injections of these agents and the relative susceptibilities of these strains to adenoma induction. The role of certain host factors in the regulation of tumor susceptibility was also tested. Homozygosity for the beige (bg) mutation had no effect on tumor numbers in C57 mice, suggesting that natural killer cells, deficient in bg/bg mice, played no major role in determining adenoma susceptibility in this strain. No correlation was found between the susceptibility of various sublines to urethan-induced lung adenoma and the reported relative tumoricidal capacities of the peritoneal macrophages from these sublines.

Links

6573537 – National Library of Medicine/PubMed

Strain Notes

Strain Note
129P3/J These mice were purchased from the Jackson Laboratory.
A.BY/SnJ The reference did not specify the gene carried over in this congenic strain.
These mice were purchased from the Jackson Laboratory.
AKR/J These mice were purchased from the Jackson Laboratory.
A/WySnJ These mice were purchased from the Jackson Laboratory.
B10.A/SgSnJ The reference did not specify the gene carried over in this congenic strain.
These mice were purchased from the Jackson Laboratory.
B10.D2-H1a/(58N)SnJ These mice were purchased from the Jackson Laboratory.
B6.Cg-Lystbg These mice were a gift from Dr. Richard Swank of the Roswell Park Memorial Institute.
C3H/HeJ These mice were purchased from the Jackson Laboratory.
C57BL/10SnJ These mice were purchased from the Jackson Laboratory.
C57BL/6J Some of these mice were purchased from the Jackson Laboratory and others were obtained from Dr. Richard Swank of RPMI.
C57BR/cdJ These mice were purchased from the Jackson Laboratory.
C57L/J These mice were purchased from the Jackson Laboratory.
HS/Ibg "HS/IBG mice were formed by crossing eight inbred strains of diverse origins, and are still maintained as a randomly bred line by avoiding inbreeding so that this genetic diversity is maximized."
LS/Ibg Selectively bred from HS/Ibg by selecting for long sleep time in response to ethanol.
NZB/BlNJ These mice were purchased from the Jackson Laboratory.
RIIIS/J These mice were purchased from the Jackson Laboratory.
SS/Ibg Selectively bred from HS/Ibg by selecting for short sleep time in response to ethanol.
SWR/J These mice were purchased from the Jackson Laboratory.

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
A/J Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

A/J Lung adenoma
  • urethane (ethyl carbamate)
  • butylated hydroxytoluene (BHT)
Lung

100

SWR/J Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

C3H/HeJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

6.7

C57BL/6J Lung adenoma
  • urethane (ethyl carbamate)
Lung

57 - 100

C57BL/6J Lung adenoma
  • urethane (ethyl carbamate)
  • butylated hydroxytoluene (BHT)
Lung

54

DBA/2J Lung adenoma
  • urethane (ethyl carbamate)
Lung

17 - 80

DBA/2J Lung adenoma
  • urethane (ethyl carbamate)
  • butylated hydroxytoluene (BHT)
Lung

16

A/WySnJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

129P3/J Lung adenoma
  • urethane (ethyl carbamate)
Lung

89

RIIIS/J Lung adenoma
  • urethane (ethyl carbamate)
Lung

70

B10.D2-H1a/(58N)SnJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

40

NZB/BlNJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

25

A.BY/SnJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

B10.A/SgSnJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

40

C57BL/10SnJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

22

C57L/J Lung adenoma
  • urethane (ethyl carbamate)
Lung

10

AKR/J Lung adenoma
  • urethane (ethyl carbamate)
Lung

0

C57BR/cdJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

0

B6.Cg-Lystbg Lung adenoma
  • urethane (ethyl carbamate)
Lung

0

C3H/HeN Lung adenoma
  • urethane (ethyl carbamate)
Lung

36

C57BL/10ScN Lung adenoma
  • urethane (ethyl carbamate)
Lung

30

C3H/2Ibg Lung adenoma
  • urethane (ethyl carbamate)
Lung

0 - 17

C3H/2Ibg Lung adenoma
  • urethane (ethyl carbamate)
  • butylated hydroxytoluene (BHT)
Lung

0

C57BL/6By Lung adenoma
  • urethane (ethyl carbamate)
Lung

33

BALB/cJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

HS/Ibg Lung adenoma
  • urethane (ethyl carbamate)
Lung

35

SS/Ibg Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

LS/Ibg Lung adenoma
  • urethane (ethyl carbamate)
Lung

90

BALB/cByJ Lung adenoma
  • urethane (ethyl carbamate)
Lung

80 - 100

BALB/cByJ Lung adenoma
  • urethane (ethyl carbamate)
  • butylated hydroxytoluene (BHT)
Lung

96 - 100

(SWR/J x BALB/cBy)F1 Lung adenoma
  • urethane (ethyl carbamate)
Lung

92

(C3H/2Ibg x A/J)F1 Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

(DBA/2J x A/J)F1 Lung adenoma
  • urethane (ethyl carbamate)
Lung

100

(C57BL/6J x A/J)F1 Lung adenoma
  • urethane (ethyl carbamate)
Lung

100