Reference SummaryDeMayo FJ, Am J Physiol 1991 Aug;261(2 Pt 1):L70-6

Title

Expression of SV40 T antigen under control of rabbit uteroglobin promoter in transgenic mice.

Authors

DeMayo FJ; Finegold MJ; Hansen TN; Stanley LA; Smith B; Bullock DW

Journal

Am J Physiol

Volume

261

Issue

2 Pt 1

Year

1991

Pages

L70-6

Abstract

The rabbit uteroglobin gene is expressed in the lungs and reproductive tracts of male and female rabbits. To examine whether the promoter region of the uteroglobin gene could be used to target a heterologous gene to the lungs of transgenic mice, a fusion gene consisting of 3.3 kb of the 5'-flanking region of the rabbit uteroglobin gene and the large T antigen gene of the SV40 virus was constructed and microinjected into the pronuclei of one-cell mouse embryos. Eleven founder transgenic mice (5 female and 6 male) were generated. Seven of these mice developed bronchioalveolar neoplasms. Four of the founder males also developed primitive undifferentiated urogenital tract tumors. One founder female and one female offspring of a founder male developed glandular paraovarian tumors. Northern analysis revealed that the predominant site of expression of the transgene was the lung. Immunohistochemical staining showed T antigen predominantly in epithelial cells lining the bronchioles, the submucosal glands of the trachea, and the neoplasms. There appeared to be a high level of mosaicism for the transgene in the founder mice, with poor transmission of the transgene to subsequent generations. This suggests that, under the control of the uteroglobin promoter, the T antigen gene may be lethal to the fetus.

Links

1872417 – National Library of Medicine/PubMed

Strain Notes

Strain Note
FVB-Tg(UGB-TAg)2013Fjd The female founder mouse expressed the transgene at a very low level.
Transgenic progeny from founder number 2013 carrying the "UGTAg" transgene.
FVB-Tg(UGB-TAg)2031Fjd No tumors observed in this mouse up to 1 year of age.
FVB-Tg(UGB-TAg)2228Fjd No tumors observed in this mouse up to 1 year of age.
FVB-Tg(UGB-TAg)5554Fjd One progeny female carried transgene but did not transmit it to her offspring.
The male founder expressed the transgene at a high level.
There was only one transgenic progeny (a female) from the founder mouse (a male), and it did not transmit the transgene to the next generation of mice.
FVB-Tg(UGB-TAg)5786Fjd No tumors observed in this mouse up to 1 year of age.
FVB-Tg(UGB-TAg)6430Fjd The progeny generated from the founder mouse carried the transgene at only 1 insertion site (unlike the founder which carried it at 2 sites).
FVB-Tg(UGB-TAg)6430Fjd The founder mouse (male) expressed the transgene at a high level and carried the transgene at 2 insertion sites.

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB-Tg(UGB-TAg)2033Fjd Larynx hyperplasia Larynx

100

FVB-Tg(UGB-TAg)6247Fjd Lung - Club cell hyperplasia Lung - Club cell

100

FVB-Tg(UGB-TAg)2013Fjd Lung - Club cell tumor Lung - Club cell

100

FVB-Tg(UGB-TAg)2025Fjd Lung - Club cell tumor Lung - Club cell

100

FVB-Tg(UGB-TAg)2033Fjd Lung - Club cell tumor Lung - Club cell

100

FVB-Tg(UGB-TAg)5554Fjd Lung - Club cell tumor Lung - Club cell

100

FVB-Tg(UGB-TAg)5784Fjd Lung - Club cell tumor Lung - Club cell

100

FVB-Tg(UGB-TAg)6247Fjd Lung - Club cell tumor Lung - Club cell

100

FVB-Tg(UGB-TAg)6430Fjd Lung - Club cell tumor Lung - Club cell

100

FVB-Tg(UGB-TAg)6430Fjd Lung - Club cell tumor Lung - Club cell

0

FVB Lung tumor Lung

0

FVB-Tg(UGB-TAg)1651Fjd Ovary tumor Ovary

100

FVB-Tg(UGB-TAg)5554Fjd Ovary tumor Ovary

100

FVB-Tg(UGB-TAg)2033Fjd Thyroid gland carcinoma Thyroid gland

100

FVB-Tg(UGB-TAg)2025Fjd (Unspecified organ) tumor Pelvis

100

FVB-Tg(UGB-TAg)2031Fjd (Unspecified organ) tumor (Unspecified organ)

0

FVB-Tg(UGB-TAg)2033Fjd (Unspecified organ) tumor Pelvis

100

FVB-Tg(UGB-TAg)2228Fjd (Unspecified organ) tumor (Unspecified organ)

0

FVB-Tg(UGB-TAg)5554Fjd (Unspecified organ) tumor Pelvis

100

FVB-Tg(UGB-TAg)5786Fjd (Unspecified organ) tumor (Unspecified organ)

0

FVB-Tg(UGB-TAg)6430Fjd (Unspecified organ) tumor Pelvis

100

FVB-Tg(UGB-TAg)6430Fjd (Unspecified organ) tumor Pelvis

0