Reference SummaryMeuwissen R, Oncogene 2001 Oct 4;20(45):6551-8

Title

Mouse model for lung tumorigenesis through Cre/lox controlled sporadic activation of the K-Ras oncogene.

Authors

Meuwissen R; Linn SC; van der Valk M; Mooi WJ; Berns A

Journal

Oncogene

Volume

20

Issue

45

Year

2001

Pages

6551-8

Abstract

The onset of human lung cancer occurs through sequential mutations in oncogenes and tumor suppressor genes. Mutations in K-Ras play a prominent role in human non-small cell lung cancer. We have developed a mouse lung tumor model in which K-Ras can be sporadically activated through Cre-lox mediated somatic recombination. Adenoviral mediated delivery of Cre recombinase in lung epithelial cells gave rise to rapid onset of tumorigenesis, yielding pulmonary adenocarcinomas with 100% incidence after a short latency. The lung tumor lesions shared many features with human non-small cell lung cancer. Our data show that sporadic expression of the K-Ras oncogene is sufficient to elicit lung tumorigenesis. Therefore this model has many advantages over conventional transgenic models used thus far.

Links

J:72206 – MGI References
11641780 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB-Tg(GFP/KRAS2/ALPP)1Brn Lung adenocarcinoma
  • Adenovirus - recombinant, expressing Cre recombinase
Lung

100

FVB-Tg(GFP/KRAS2/ALPP)1Brn Lung adenoma
  • Adenovirus - recombinant, expressing Cre recombinase
Lung

100

FVB-Tg(GFP/KRAS2/ALPP)1Brn Lung carcinoma
  • Adenovirus - recombinant, expressing Cre recombinase
Lung

100

FVB-Tg(GFP/KRAS2/ALPP)1Brn Lung neoplasia
  • Adenovirus - recombinant, expressing Cre recombinase
Lung

80

FVB-Tg(GFP/KRAS2/ALPP)1Brn Lung tumor
  • Adenovirus - recombinant, expressing Cre recombinase
Lung

0

FVB/N Lung tumor
  • Adenovirus - recombinant, expressing Cre recombinase
Lung

0