Reference SummaryFodde R, Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8969-73

Title

 A targeted chain-termination mutation in the mouse Apc gene results in multiple intestinal tumors.

Authors

 Fodde R; Edelmann W; Yang K; van Leeuwen C; Carlson C; Renault B; Breukel C; Alt E; Lipkin M; Khan PM; Kucherlapati R

Journal

 Proc Natl Acad Sci U S A

Volume

 91

Issue

 19

Year

 1994

Pages

 8969-73

Abstract

 Germ-line mutations in the human adenomatous polyposis coli (APC) gene result in familial adenomatous polyposis, an autosomal dominant disorder characterized by the early onset of multiple adenomatous polyps in the large bowel with a high likelihood of developing colorectal carcinomas. To understand the role of APC in intestinal tumor formation, we have introduced a chain-termination mutation in the 15th exon of the mouse Apc gene and employed it to modify the endogenous gene by homologous recombination in embryonic stem cells. Mice which are heterozygous for the Apc gene modification progressively develop intestinal tumors in a manner that is similar to that observed in patients with familial adenomatous polyposis and in mice which carry a mutation called multiple intestinal neoplasia (Min). Our results indicate that the Apc gene modification is a critical event in the initiation of intestinal tumor formation and results in an autosomal dominant predisposition toward development of spontaneous colonic and intestinal tumors in mice.

Links

 J:20443 – MGI References
8090754 – National Library of Medicine/PubMed

Strain Notes

Strain Note
B6129P2F1-Apctm1Rak/+ The targeted mutation (Apctm1Rak) allele was reported to be expressed in the hepatocytes, intestine, and colon of these mice. (Expression was detected by RT-PCR. No protein product detectable by Western blotting.)

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
B6129P2F1-Apctm1Rak/+ Intestine - Large Intestine - Cecum tumor Intestine - Large Intestine - Cecum

0
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Large Intestine - Cecum tumor Intestine - Large Intestine - Cecum

0
B6129P2F1-Apctm1Rak/+ Intestine - Large Intestine - Colon - Distal adenoma Intestine - Large Intestine - Colon - Distal

9.1
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Large Intestine - Colon - Distal polyp Intestine - Large Intestine - Colon - Distal

56
B6129P2F1-Apctm1Rak/+ Intestine - Large Intestine - Colon - Distal polyp Intestine - Large Intestine - Colon - Distal

55
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Large Intestine - Colon - Medial polyp Intestine - Large Intestine - Colon - Medial

11
B6129P2F1-Apctm1Rak/+ Intestine - Large Intestine - Colon - Medial polyp Intestine - Large Intestine - Colon - Medial

9.1
B6129P2F1-Apctm1Rak/+ Intestine - Large Intestine - Colon - Proximal adenoma Intestine - Large Intestine - Colon - Proximal

9.1
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Large Intestine - Colon - Proximal polyp Intestine - Large Intestine - Colon - Proximal

11
B6129P2F1-Apctm1Rak/+ Intestine - Large Intestine - Colon - Proximal polyp Intestine - Large Intestine - Colon - Proximal

27
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Small Intestine - Duodenum adenoma Intestine - Small Intestine - Duodenum

11
B6129P2F1-Apctm1Rak/+ Intestine - Small Intestine - Duodenum adenoma Intestine - Small Intestine - Duodenum

36
B6129P2F1-Apctm1Rak/+ Intestine - Small Intestine - Duodenum carcinoma Intestine - Small Intestine - Duodenum

27
B6129P2F1-Apctm1Rak/+ Intestine - Small Intestine - Ileum adenoma Intestine - Small Intestine - Ileum

9.1
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Small Intestine - Ileum carcinoma Intestine - Small Intestine - Ileum

11
B6129P2F1-Apctm1Rak/+ Intestine - Small Intestine - Ileum carcinoma Intestine - Small Intestine - Ileum

18
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Small Intestine - Jejunum adenoma Intestine - Small Intestine - Jejunum

11
B6129P2F1-Apctm1Rak/+ Intestine - Small Intestine - Jejunum adenoma Intestine - Small Intestine - Jejunum

18
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Intestine - Small Intestine - Jejunum carcinoma Intestine - Small Intestine - Jejunum

11
B6129P2F1-Apctm1Rak/+ Intestine - Small Intestine - Jejunum carcinoma Intestine - Small Intestine - Jejunum

27
(C57BL/6 x 129P2/OlaHsd)F1 Intestine tumor Intestine

0
B6129P2F1-Apctm1Rak/+ Stomach - Glandular adenoma Stomach - Glandular

13
B6129P2F1-Apctm1Rak/+ Stomach - Glandular carcinoma Stomach - Glandular

13
C57BL/6 +/+ : 129P2/OlaHsd-Apctm1Rak/+ Stomach - Glandular tumor Stomach - Glandular

0
(C57BL/6 x 129P2/OlaHsd)F1 Stomach tumor Stomach

0