Reference SummaryZhang Y, Int J Radiat Biol 1998 Jul;74(1):43-51
Title |
Detection of codon 12 point mutations of the K-ras gene from mouse lung adenocarcinoma by 'enriched' PCR. |
Authors |
Zhang Y; Woloschak GE |
Journal |
Int J Radiat Biol |
Volume |
74 |
Issue |
1 |
Year |
1998 |
Pages |
43-51 |
Abstract |
PURPOSE: Recent studies have shown that chemical carcinogens induce a high frequency of point mutations in the K-ras oncogene from mouse lung tumours at codons 12, 13 and 61. These experiments were performed to identify K-ras mutations in tissues from control and radiation-exposed mice. MATERIALS AND METHODS: By modifying the technique of the 'enriched' polymerase chain reaction (PCR), it was possible to detect point mutations at codon 12 of the K-ras oncogene from 25-year-old paraffin-embedded normal lungs and lung adenocarcinomas from mice exposed to radiation. Together, a total of 120 lung tissues were screened for point mutations at codon 12 of the K-ras oncogene in this study. RESULTS: A significant increase in K-ras codon 12 point mutations was observed in the normal lungs from mice exposed to 24 once-weekly neutron irradiations (100%), compared with normal lungs from mice with sham-irradiation (50%) (p<0.05). Lung adenocarcinomas from mice receiving 24 once-weekly neutron irradiations also had a significantly higher frequency of K-ras codon 12 point mutations (100%) than the lung adenocarcinomas of mice receiving 24 or 60 once-weekly gamma-ray irradiations (50%), but the higher frequency was not significantly different from that in spontaneous lung adenocarcinomas from mice (75%; p > 0.05). The validity of the technique was confirmed by sequencing two of the mutants. In doing so, a K-ras 13(Asp) point mutation was observed. CONCLUSIONS: The data suggest that high-linear energy transfer (LET) neutron radiation was more effective than low-LET gamma-rays in inducing K-ras point mutations at codon 12 in the lungs of B6CF1 mice. |
Links |
J:49183 – MGI References 9687974 – National Library of Medicine/PubMed |
| Strain | Model Name | Treatment Agent(s) | Organ Affected | Frequency | Model Details |
|---|---|---|---|---|---|
| (C57BL/6 x BALB/c)F1 | Leukocyte lymphoma | Leukocyte |
very high |
||
| (C57BL/6 x BALB/c)F1 | Leukocyte lymphoma |
|
Leukocyte |
very high |
|
| (C57BL/6 x BALB/c)F1 | Leukocyte lymphoma |
|
Leukocyte |
very high |
|
| (C57BL/6 x BALB/c)F1 | Lung adenocarcinoma | Lung |
observed |
||
| (C57BL/6 x BALB/c)F1 | Lung adenocarcinoma |
|
Lung |
observed |
|
| (C57BL/6 x BALB/c)F1 | Lung adenocarcinoma |
|
Lung |
observed |