Reference SummaryEastin WC, Toxicol Pathol 2001;29 Suppl():60-80
Title |
Tg.AC genetically altered mouse: assay working group overview of available data. |
Authors |
Eastin WC; Mennear JH; Tennant RW; Stoll RE; Branstetter DG; Bucher JR; McCullough B; Binder RL; Spalding JW; Mahler JF |
Journal |
Toxicol Pathol |
Volume |
29 Suppl |
Issue |
|
Year |
2001 |
Pages |
60-80 |
Abstract |
In a Government/Industry/Academic partnership to evaluate alternative approaches to carcinogenicity testing, 21 pharmaceutical agents representing a variety of chemical and pharmacological classes and possessing known human and or rodent carcinogenic potential were selected for study in several rodent models. The studies from this partnership project, coordinated by the International Life Sciences Institute, provide additional data to better understand the models' limitations and sensitivity in identifying carcinogens. The results of these alternative model studies were reviewed by members of Assay Working Groups (AWG) composed of scientists from government and industry with expertise in toxicology, genetics, statistics, and pathology. The Tg.AC genetically manipulated mouse was one of the models selected for this project based on previous studies indicating that Tg.AC mice seem to respond to topical application of either mutagenic or nonmutagenic carcinogens with papilloma formation at the site of application. This communication describes the results and AWG interpretations of studies conducted on 14 chemicals administered by the topical and oral (gavage and/or diet) routes to Tg.AC genetically manipulated mice. Cyclosporin A, an immunosuppresant human carcinogen, ethinyl estradiol and diethylstilbestrol (human hormone carcinogens) and clofibrate, an hepatocarcinogenic peroxisome proliferator in rodents, were considered clearly positive in the topical studies. In the oral studies, ethinyl estradiol and diethylstilbestrol were negative, cyclosporin was considered equivocal, and results were not available for the clofibrate study. Of the 3 genotoxic human carcinogens (phenacetin, melphalan, and cyclophosphamide), phenacetin was negative by both the topical and oral routes. Melphalan and cyclophosphamide are, respectively, direct and indirect DNA alkylating agents and topical administration of both caused equivocal responses. With the exception of clofibrate, Tg.AC mice did not exhibit tumor responses to the rodent carcinogens that were putative human noncarcinogens, (di(2-ethylhexyl) phthalate, methapyraline HCl, phenobarbital Na, reserpine, sulfamethoxazole or WY-14643, or the nongenotoxic, noncarcinogen, sulfisoxazole) regardless of route of administration. Based on the observed responses in these studies, it was concluded by the AWG that the Tg.AC model was not overly sensitive and possesses utility as an adjunct to the battery of toxicity studies used to establish human carcinogenic risk. |
Links |
J:73017 – MGI References 11695563 – National Library of Medicine/PubMed |
Strain | Model Name | Treatment Agent(s) | Organ Affected | Frequency | Model Details |
---|---|---|---|---|---|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Erythrocyte leukemia - erythroleukemia | Erythrocyte |
0 - 3.3 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Erythrocyte leukemia - erythroleukemia | Erythrocyte |
6.7 - 8.3 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Erythrocyte leukemia - erythroleukemia |
|
Erythrocyte |
0 - 26.7 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach hyperplasia - squamous |
|
Forestomach |
86.7 - 93.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach hyperplasia - squamous | Forestomach |
86.7 - 100 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach hyperplasia - squamous |
|
Forestomach |
86.7 - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach lesion |
|
Forestomach |
100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach lesion | Forestomach |
21.4 - 46.2 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach lesion |
|
Forestomach |
30.8 - 63.6 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach papilloma | Forestomach |
44.9 - 66.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach papilloma | Forestomach |
21.7 - 25.4 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach papilloma |
|
Forestomach |
20 - 60 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach squamous cell papilloma | Forestomach |
57.1 - 80 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach squamous cell papilloma |
|
Forestomach |
92.9 - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor | Forestomach |
0 - 80 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
77.8 - 93.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
11.1 - 40 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
46.7 - 73.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor | Forestomach |
0 - 42.9 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
0 - 20 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
53.3 - 86.7 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
0 - 26.7 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
45.5 - 83.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
41.7 - 60 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
0 - 62.5 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
0 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
0 - 71.4 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Forestomach tumor |
|
Forestomach |
30.8 - 60 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Leukocyte lymphoma | Leukocyte |
0.6 - 1.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Leukocyte lymphoma | Leukocyte |
0 - 1.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Leukocyte tumor | Leukocyte |
0 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Leukocyte tumor |
|
Leukocyte |
0 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Leukocyte tumor |
|
Leukocyte |
0 - 23.1 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Liver hepatocellular adenoma |
|
Liver |
13.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Liver hyperplasia - oval cell |
|
Liver |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Lung - Alveolus hyperplasia - focal |
|
Lung - Alveolus |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Lung tumor | Lung |
1.1 - 2.2 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Lung tumor | Lung |
1.7 - 5.0 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Nose adenoma |
|
Bone - Jaw - Maxilla |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Ovary - Corpus luteum hyperplasia |
|
Ovary - Corpus luteum |
observed |
|
FVB/NTac | Ovary - Corpus luteum hyperplasia |
|
Ovary - Corpus luteum |
observed |
|
FVB/NTac | Ovary - Corpus luteum hyperplasia |
|
Ovary - Corpus luteum |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Salivary gland carcinoma | Salivary gland |
1.7 - 2.8 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Salivary gland carcinoma | Salivary gland |
0 - 1.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dermis papilloma |
|
Vulva |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region papilloma |
|
Skin - Dorsal region |
very high - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region papilloma | Skin - Dorsal region |
6.7 - 40 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region papilloma |
|
Skin - Dorsal region |
33.3 - 73.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region papilloma |
|
Skin - Dorsal region |
0 - 80 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
60 - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor | Skin - Dorsal region |
0 - 20 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 86.7 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
33.3 - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor | Skin - Dorsal region |
0 - 21.4 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 20 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
4.2 - 10.7 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 10 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 27.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
86.7 - 93.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 6.7 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
6.7 - 26.7 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 13.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 40 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin - Dorsal region tumor |
|
Skin - Dorsal region |
0 - 20 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin papilloma |
|
Skin |
85 - 88 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin papilloma |
|
Skin |
93 - 96 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin squamous cell papilloma | Scrotum |
6.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin squamous cell papilloma |
|
Scrotum |
13.3 - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin squamous cell papilloma | Vulva |
13.3 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin squamous cell papilloma |
|
Vulva |
observed - 90.9 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor | Skin |
0 - 40 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor | Skin |
0 - 1.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
Skin |
0 - 33.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
Skin |
6.7 - 20 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor | (Unspecified organ) |
0 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
(Unspecified organ) |
0 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor | Vulva |
6.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
Vulva |
20 - 80 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
Skin |
20 - 80 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
Skin |
93.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
Skin |
13.3 - 100 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor |
|
Vagina |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor - squamous | Skin |
26.7 - 28.6 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Skin tumor - squamous |
|
Skin |
13.3 - 93.3 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Tooth tumor | Tooth |
7.0 - 30.0 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Tooth tumor | Tooth |
18.3 - 26.7 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Tooth tumor |
|
Tooth |
7.0 - 30.0 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | Uterus - Endometrium hyperplasia - cystic |
|
Uterus - Endometrium |
observed |
|
FVB/NTac | Uterus - Endometrium hyperplasia - cystic |
|
Uterus - Endometrium |
observed |
|
FVB/NTac | Uterus - Endometrium hyperplasia - cystic |
|
Uterus - Endometrium |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | (Unspecified organ) hyperplasia |
|
(Unspecified organ) |
observed |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | (Unspecified organ) hyperplasia |
|
(Unspecified organ) |
observed |
|
FVB/NTac | (Unspecified organ) lesion |
|
(Unspecified organ) |
0 |
|
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | (Unspecified organ) tumor | (Unspecified organ) |
0 - 2.2 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | (Unspecified organ) tumor | (Unspecified organ) |
1.7 - 3.3 |
||
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed | (Unspecified organ) tumor |
|
(Unspecified organ) |
0 |