Pathology Image Detail

Caption	Macrocyst, immunohistochemistry for alpha smooth muscle actin: there is no evidence of myoepithelial differentiation withing the macrocyst (right portion of the photomicrograph). A discrete layer of myoepithelial cells lines acini and ducts (*) in the mammary parenchyma adjacent to the macrocyst. Lactation is present in the mammary parenchymal adjacent to the macrocyst.
Description	Macrocyst, mammary gland
Age at Necropsy	unknown
Contributor	Mikaelian I (J:94320)
Pathologist	Mikaelian I (J:94320)
Method	IHC for alpha smooth muscle actin

Image Probes
Type: antibody	Name anti-alpha smooth muscle actin (A2547) Target alpha-smooth muscle actin, N-terminus Supplier Sigma-Aldrich Notes Monoclonal anti-Alpha Smooth Muscle Actin antibody produced in mouse (raised against N-terminal synthetic decapeptide of alpha-smooth muscle actin). The antibody (also known as anti-a-Sm-1) is specific for the single isoform of alpha-smooth muscle actin.

General Note: Hemizygous mice. The transgene was generated in a (C57BL/6 x DBA/2)F2 genetic background and was subsequently backcrossed to FVB/NJ.