PDX Model DetailsTM00089

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Model Details

Model ID (Previous ID)
TM00089 (BR0620F)
Primary Site
Breast
Tumor Site
Breast
Tumor Type
Primary Malignancy
Initial Diagnosis
invasive ductal carcinoma
Final Diagnosis
invasive ductal carcinoma
Stage/Grade
AJCC IIA / Grade:3

Publications citing this model

Arnold, KM; et al. Activation of Inflammatory Responses Correlate With Hedgehog Activation and Precede Expansion of Cancer Stem-Like Cells in an Animal Model of Residual Triple Negative Breast Cancer after Neoadjuvant Chemotherapy. Cancer Studies and Molecular Medicine 2015;2(2), p80-86. DOI:10.17140/CSMMOJ-2-112

Patient

Sex
Female
Age
68
Race/Ethnicity
Asian or Pacific Islander / Not Hispanic or Latino
Treatment Naïve
Yes

Engraftment Host

Strain
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
(aka NSG or NOD Scid gamma)
Sample Type
Surgical resection (fragment)

Tumor Mutation Burden

Sample
Sample from passage P0 has a TMB score of 3.23
TMB Range
0.0-291.5 (across all JAX PDX models; TMB > 22 is considered high)

Microsatellite Instability

Sample
Sample:BR0620F037P0     Score:MSI-S

Variant Summary

Gene Expression

Platforms: RNA_Seq, hg10st

Hatched bars indicate genes with expression levels determined to be mildly affected by alignment calculations. Details.

Gene CNV

CNV Plots

Model Characterization

Histology: Tumor Markers:
Patient and PDX tumor markers differ. IHC results show PDX model TM00089 is ER positive/PR negative/ERBB2 negative for lineage 039 while it is ER negative/PR negative/ERBB2 negative for lineage 037. Patient tumor reported as ER positive/PR negative/ERBB2 negative.
Summary:
P2 and P5 pathology consistent with poorly differentiated carcinoma.
Pathologist:
Dr. Regina Gandour-Edwards, University of California, Davis.

Patient tumor for PDX model TM00089.

Patient tumor for PDX model TM00089.

Patient tumor for PDX model TM00089.

Patient tumor for PDX model TM00089; Ki67 marker

Patient tumor for PDX model TM00089; PR marker (negative)

Patient tumor for PDX model TM00089; ER marker (positive).

P0 tumor #037 for PDX model TM00089; estradiol supplemented

P0 tumor #037 for PDX model TM00089, estradiol supplemented, stained for Ki67. Staining was positive.

P0 tumor #037 for PDX model TM00089; estradiol supplemented; ER marker

P1 tumor for PDX model TM00089; estradiol supplemented

P1 tumor for PDX model TM00089; estradiol supplemented; PR marker (negative)

P1 tumor for PDX model TM00089; estradiol supplemented; ER marker (negative)

P1 tumor #039 from PDX model TM00089.

P1 tumor #039 from PDX model TM00089; PR marker (negative)

P1 tumor #039 for PDX model TM00089; ER marker (focal positive)

P2 tumor F037 ID013 for PDX model TM00089 stained for H&E.

P2 tumor F037 ID013 for PDX model TM00089 stained for AE1AE3. Staining was positive.

P2 F037 ID013 tumor for PDX model TM00089 stained for CD45. Staining was negative.

P2 F037 ID013 tumor for PDX model TM00089 stained for Ki67. Staining was positive.

P2 F037 ID013 tumor for PDX model TM00089 stained for ER. Staining was negative.

P2 F037 ID013 tumor for PDX model TM00089 stained for PR. Staining was negative.

P2 F037 ID013 tumor for PDX model TM00089 stained for ERBB2 (HER2). Staining was negative.

P5 F037 ID027 tumor for PDX model TM00089 stained for H&E.

P5 F037 ID027 tumor for PDX model TM00089 stained for AE1/AE3. Staining was positive.

P5 F037 ID027 tumor for PDX model TM00089 stained for CD45. Staining was negative.

P5 F037 ID027 tumor for PDX model TM00089 stained for Ki67. Staining was positive.

P5 F037 ID027 tumor for PDX model TM00089 stained for AR. Staining was negative.

P5 F037 ID027 tumor for PDX model TM00089 stained for ER. Staining was negative.

P5 F037 ID027 tumor for PDX model TM00089 stained for ERBB2 (HER2). Staining was negative.

P5 F037 ID027 tumor for PDX model TM00089 stained for PR. Staining was negative.
Circos Plots:

Circos plot for P0 tumor #037 from PDX model TM00089 (aka, BR0620)

Circos plot for P0 tumor #039 from PDX model TM00089 (aka, BR0620)

Circos plot for P1 tumor #001 from PDX model TM00089 (aka, BR0620F)

Dosing Studies