Reference SummaryMoser AR, J Cell Biol 1992 Mar;116(6):1517-26
Title |
The Min (multiple intestinal neoplasia) mutation: its effect on gut epithelial cell differentiation and interaction with a modifier system. | ||||||
Authors |
Moser AR; Dove WF; Roth KA; Gordon JI | ||||||
Journal |
J Cell Biol | ||||||
Volume |
116 | ||||||
Issue |
6 | ||||||
Year |
1992 | ||||||
Pages |
1517-26 | ||||||
Abstract |
Min is a fully penetrant dominant mutation that leads to the development of multiple intestinal adenomas throughout the duodenal-to-colonic axis. Min/+ C57BL6/J mice have an average life-span of 120 d. Multi-label immunocytochemical studies of these lesions demonstrate patches of differentiated enterocytes, and scattered enteroendocrine, goblet and Paneth cells. Expression of endogenous marker genes within these differentiated cells can be directly correlated with the position occupied by the adenoma along the duodenal-to-colonic axis and mirrors the regional differentiation of the normal gut epithelium. The presence of multiple lineages in adenomas together with their retention of spatial information suggests that tumorigenesis in Min/+ mice may be initiated in a multipotent stem cell normally located at the base of intestinal crypts. To study the time-dependent properties of these tumors, genetic conditions were sought in which Min/+ animals could survive for up to 300 d. Min is fully penetrant in hybrids with either AKR/J or MA/MyJ. However, the hybrids demonstrate a reduction in the number of intestinal adenomas. Preliminary backcross analysis is consistent with a single major modifier locus unlinked to Min in both the AKR/J and MA/MyJ strains. The increased lifespan of the hybrid animals is also associated with the development of invasive tumors. New tumors do not arise continuously over the lifespan of these animals; instead all adenomas appear to be established by 100 d of age or sooner. These studies indicate that the Min/+ mouse is a powerful model system for analyzing the mechanisms that establish and maintain a balance between proliferation and differentiation in the continuously renewing gut epithelium and for an assessment of the multi-step hypothesis of intestinal neoplasia. | ||||||
Links |
J:1879 – MGI References 1541640 – National Library of Medicine/PubMed |
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Strain Notes
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| Strain | Model Name | Treatment Agent(s) | Organ Affected | Frequency | Model Details |
|---|---|---|---|---|---|
| C57BL/6J-ApcMin/+ | Intestine - Large Intestine - Colon - Distal adenoma | Intestine - Large Intestine - Colon - Distal |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine - Large Intestine - Colon - Proximal adenoma | Intestine - Large Intestine - Colon - Proximal |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine - Large Intestine - Colon adenoma | Intestine - Large Intestine - Colon |
observed |
||
| MAB6F1-ApcMin/+ | Intestine - Large Intestine - Colon adenoma | Intestine - Large Intestine - Colon |
observed |
||
| AKB6F1-ApcMin/+ | Intestine - Large Intestine - Colon adenoma | Intestine - Large Intestine - Colon |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine - Large Intestine adenoma | Intestine - Large Intestine |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine - Small Intestine - Duodenum adenoma | Intestine - Small Intestine - Duodenum |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine - Small Intestine - Ileum adenoma | Intestine - Small Intestine - Ileum |
observed |
||
| (AKMAF1 x C57BL/6J)-ApcMin/+ | Intestine - Small Intestine - Jejunum adenocarcinoma | Intestine - Small Intestine - Jejunum |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine - Small Intestine - Jejunum adenoma | Intestine - Small Intestine - Jejunum |
observed |
||
| (AKMAF1 x C57BL/6J)-ApcMin/+ | Intestine - Small Intestine adenocarcinoma | Intestine - Small Intestine |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine - Small Intestine adenoma | Intestine - Small Intestine |
observed |
||
| MAB6F1-ApcMin/+ | Intestine - Small Intestine adenoma | Intestine - Small Intestine |
observed |
||
| AKB6F1-ApcMin/+ | Intestine - Small Intestine adenoma | Intestine - Small Intestine |
observed |
||
| C57BL/6J-ApcMin/+ | Intestine adenocarcinoma | Intestine |
0 |
||
| C57BL/6J-ApcMin/+ | Intestine adenoma | Intestine |
43 |
||
| MAB6F1-ApcMin/+ | Intestine adenoma | Intestine |
64 |
||
| AKB6F1-ApcMin/+ | Intestine adenoma | Intestine |
46 - 57 |
||
| MA;B6-ApcMin/+ | Intestine adenoma | Intestine |
observed |
||
| AK;B6-ApcMin/+ | Intestine adenoma | Intestine |
observed |