Reference SummaryBlackwell BN, Toxicol Pathol 1995 Sep-Oct;23(5):570-82

Title

Longevity, body weight, and neoplasia in ad libitum-fed and diet-restricted C57BL6 mice fed NIH-31 open formula diet.

Authors

Blackwell BN; Bucci TJ; Hart RW; Turturro A

Journal

Toxicol Pathol

Volume

23

Issue

5

Year

1995

Pages

570-82

Abstract

Groups of C57BL6 mice of each sex were assigned to one of 2 dietary regimens, ad libitum (AL) or dietary restriction (DR), to study effects of food restriction on body weight, survival, and neoplasia. The AL and DR groups were subdivided into a scheduled sacrifice group for examination at 6-mo intervals, and a lifetime group to provide longevity data. Necropsies and microscopic examinations were conducted on 911 animals. In the lifetime group food consumption averaged 33.6 and 34.4 g per week by AL males and AL females, respectively; the DR counterparts were given 40% less. The diet contained 4.35 kcal/g. The average lifetime body weights were 34.8, 26.8, 22.6, and 21.6 g for AL males, AL females, DR males, and DR females, respectively, and their age at 50% survival was 27.5, 26.9, 31.7, and 33.5 mo. Maximal lifespan was increased 18% in DR males and females. Lifetime incidence of tumor-bearing mice was 89% and 86% for AL males and females, versus 64% for each sex of DR mice. Dramatic reduction occurred in female DR mice in lymphoma (9% vs 29%), pituitary neoplasms (1% vs 37%), and thyroid neoplasms (0.4% vs 8%). In males, hepatocellular tumors were reduced to 1% from 10% by DR. In contrast, the incidence of histiocytic sarcoma was increased in DR females and unaffected in DR males. Tumor onset was delayed in DR animals; 87% of all neoplasms in males and 95% in females had occurred in the AL mice by 24 mo, whereas the DR animals had only 52% and 39% of their lifetime incidence, respectively, by that age. This study provided comparative AL and DR data from C57BL6 mice examined randomly at 6-mo intervals (cross-sectional group) in parallel with data from animals in similar cohort that was unsampled and allowed to succumb naturally (longevity group). Dietary restriction reduced the lifetime percentage of tumor-bearing animals and the number of tumors per animal, and delayed the age at onset of most neoplasms.

Links

8578100 – National Library of Medicine/PubMed

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6JNia Adrenal gland - Medulla pheochromocytoma Adrenal gland - Medulla

1.2 - 2.6

C57BL/6NNia Adrenal gland - Medulla pheochromocytoma
  • calorie restricted
Adrenal gland - Medulla

0 - 6.7

C57BL/6JNia Adrenal gland adenoma Adrenal gland

3.7

C57BL/6NNia Adrenal gland adenoma
  • calorie restricted
Adrenal gland

0.5

C57BL/6NNia Adrenal gland tumor
  • calorie restricted
Adrenal gland

0.6

C57BL/6JNia Blood vessel tumor Blood vessel

0 - 7.1

C57BL/6NNia Blood vessel tumor
  • calorie restricted
Blood vessel

0 - 6.7

C57BL/6NNia Bone - Nose osteosarcoma
  • calorie restricted
Bone - Nose

1.1

C57BL/6NNia Bone - Skull osteoma
  • calorie restricted
Bone - Skull

0.6

C57BL/6NNia Bone osteosarcoma
  • calorie restricted
Bone

0 - 7.1

C57BL/6NNia Connective tissue - Fibroblast fibrosarcoma
  • calorie restricted
Skin

0.6

C57BL/6JNia Eye - Harderian gland adenoma Eye - Harderian gland

0 - 7.1

C57BL/6NNia Eye - Harderian gland adenoma
  • calorie restricted
Eye - Harderian gland

0 - 13

C57BL/6JNia Gallbladder papilloma Gallbladder

0 - 7.1

C57BL/6NNia Intestine - Small Intestine - Duodenum polyp
  • calorie restricted
Intestine - Small Intestine - Duodenum

0 - 13

C57BL/6NNia Intestine - Small Intestine - Ileum polyp
  • calorie restricted
Intestine - Small Intestine - Ileum

0.6

C57BL/6JNia Intestine - Small Intestine tumor Intestine - Small Intestine

0

C57BL/6JNia Kidney adenoma Kidney

1.2

C57BL/6NNia Kidney adenoma
  • calorie restricted
Kidney

0.5

C57BL/6NNia Kidney papilloma
  • calorie restricted
Kidney

0.5

C57BL/6JNia Kidney tumor Kidney

0.6

C57BL/6JNia Leukocyte - Monocyte - Macrophage - Histiocyte histiocytic sarcoma Leukocyte - Monocyte - Macrophage - Histiocyte

0 - 55

C57BL/6NNia Leukocyte - Monocyte - Macrophage - Histiocyte histiocytic sarcoma
  • calorie restricted
Leukocyte - Monocyte - Macrophage - Histiocyte

0 - 50

C57BL/6JNia Leukocyte - Myelocyte (Granulocyte) leukemia - myelocytic Leukocyte - Myelocyte (Granulocyte)

0.9

C57BL/6JNia Leukocyte lymphoma Leukocyte

0 - 60

C57BL/6NNia Leukocyte lymphoma
  • calorie restricted
Leukocyte

0 - 27

C57BL/6JNia Liver - Sinusoid - Ito cell tumor - benign Liver - Sinusoid - Ito cell

0.6

C57BL/6JNia Liver hepatocellular adenoma Liver

0 - 7.1

C57BL/6NNia Liver hepatocellular adenoma
  • calorie restricted
Liver

0.5 - 1.1

C57BL/6JNia Liver hepatocellular carcinoma Liver

0 - 7.1

C57BL/6NNia Liver hepatocellular carcinoma
  • calorie restricted
Liver

0.6 - 1.1

C57BL/6JNia Lung adenoma Lung

0 - 14

C57BL/6NNia Lung adenoma
  • calorie restricted
Lung

0 - 6.7

C57BL/6JNia Lung carcinoma Lung

0 - 7.1

C57BL/6NNia Lung carcinoma
  • calorie restricted
Lung

0.5 - 0.6

C57BL/6JNia Mammary gland carcinoma Mammary gland

3.4

C57BL/6NNia Mammary gland carcinoma
  • calorie restricted
Mammary gland

0.6

C57BL/6JNia Mammary gland tumor - mixed Mammary gland

1.7

C57BL/6JNia Ovary adenoma Ovary

0.9

C57BL/6NNia Ovary adenoma
  • calorie restricted
Ovary

1.1

C57BL/6NNia PNS - Schwann cell schwannoma
  • calorie restricted
Nose

0 - 7.1

C57BL/6NNia PNS - Schwann cell schwannoma
  • calorie restricted
PNS - Schwann cell

0.6

C57BL/6NNia PNS - Schwann cell schwannoma
  • calorie restricted
Eye

0.6

C57BL/6JNia PNS - Schwann cell schwannoma Urinary bladder

0.6

C57BL/6NNia Pancreas - Islet of Langerhans adenoma
  • calorie restricted
Pancreas - Islet of Langerhans

0.5

C57BL/6JNia Paranasal sinus polyp Paranasal sinus

0.6

C57BL/6JNia Parathyroid gland adenoma Parathyroid gland

0 - 7.1

C57BL/6NNia Parathyroid gland adenoma
  • calorie restricted
Parathyroid gland

0.5

C57BL/6JNia Pituitary gland adenoma Pituitary gland

0 - 64

C57BL/6NNia Pituitary gland adenoma
  • calorie restricted
Pituitary gland

0 - 6.7

C57BL/6JNia Pituitary gland tumor Pituitary gland

0

C57BL/6NNia Pituitary gland tumor
  • calorie restricted
Pituitary gland

0

C57BL/6NNia Skin - Hair follicle - Basal cell trichoepithelioma
  • calorie restricted
Skin - Hair follicle - Basal cell

0.6

C57BL/6JNia Stomach squamous cell papilloma Stomach

1.2

C57BL/6JNia Thymus thymoma Thymus

1.2

C57BL/6JNia Thyroid gland - Follicular cell adenoma Thyroid gland - Follicular cell

0 - 29

C57BL/6JNia Thyroid gland - Follicular cell carcinoma Thyroid gland - Follicular cell

1.7

C57BL/6NNia Thyroid gland - Follicular cell carcinoma
  • calorie restricted
Thyroid gland - Follicular cell

0.6

C57BL/6NNia Thyroid gland tumor
  • calorie restricted
Thyroid gland

0

C57BL/6NNia Urethra papilloma
  • calorie restricted
Urethra

0.5

C57BL/6NNia Urinary bladder papilloma
  • calorie restricted
Urinary bladder

0.5

C57BL/6JNia Uterus adenoma Uterus

1.7

C57BL/6JNia Uterus polyp Uterus

0 - 14

C57BL/6NNia Uterus polyp
  • calorie restricted
Uterus

0 - 6.7

C57BL/6NNia Uterus tumor
  • calorie restricted
Uterus

0

C57BL/6JNia Vagina carcinoma Vagina

0 - 7.1

C57BL/6NNia Vagina tumor - granular cell
  • calorie restricted
Vagina

0.6

C57BL/6JNia (Unspecified organ) tumor (Unspecified organ)

12 - 100

C57BL/6NNia (Unspecified organ) tumor
  • calorie restricted
(Unspecified organ)

0 - 69