Reference Summaryvan Wezel T, Nat Genet 1996 Dec;14(4):468-70

Title

 Gene interaction and single gene effects in colon tumour susceptibility in mice [see comments]

Authors

 van Wezel T; Stassen AP; Moen CJ; Hart AA; van der Valk MA; Demant P

Journal

 Nat Genet

Volume

 14

Issue

 4

Year

 1996

Pages

 468-70

Abstract

 To dissect the multigenic control of colon tumour susceptibility in the mouse we used the set of 20 CcS/Dem (CcS) recombinant congenic (RC) strains. Each CcS strain carries a unique, random subset of approximately 12.5% of the genome of strain STS/A (STS) on the genetic background of BALB/cHeA (BALB/c). Previously, applying a protocol of 26 injections of 1,2-dimethylhydrazine (DMH), we detected two susceptibility loci, Scc1 and Scc2, on chromosome 2 (refs 4, 5). Using a shorter tumour-induction procedure, combining DMH and N-ethyl-N-nitrosourea (ENU) treatment, we demonstrate that BALB/c, STS and most CcS strains are relatively resistant. The strain CcS-19, however, is susceptible, probably due to a combination of BALB/c and STS alleles at several loci. Analysis of 192 (BALB/c x CcS-19) F2 mice revealed, in addition to the Scc1/Scc2 region, three new susceptibility loci: Scc3 on chromosome 1, Scc4 on chromosome 17 and Scc5 on chromosome 18. Scc4 and Scc5 have no apparent individual effect, but show a strong reciprocal interaction. Their BALB/c and STS alleles are not a priori susceptible or resistant but the genotype at one locus determines the effect of the allele at the second locus and vice versa. These findings and the accompanying paper on lung tumour susceptibility show that interlocus interactions are likely to be an important component of tumour susceptibility.

Links

 J:36996 – MGI References
8944029 – National Library of Medicine/PubMed

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
BALB/cHeA Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

43 - 58
STS/A Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

17 - 27
CcS1/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

12.5
CcS2/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

28 - 29
CcS3/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

11
CcS4/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

10 - 38
CcS5/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

40 - 50
CcS6/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

12.5 - 30
CcS7/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

43 - 53
CcS8/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

11 - 29
CcS9/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

36
CcS10/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

14 - 21
CcS11/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

20 - 24
CcS12/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

25 - 31
CcS13/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

11 - 29
CcS14/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

35 - 43
CcS15/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

12 - 22
CcS16/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

59 - 86
CcS17/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

44 - 53
CcS18/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

67
CcS19/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

82 - 83
CcS20/Dem Intestine - Large Intestine - Colon tumor
  • 1,2-dimethylhydrazine (DMH)
  • N-ethyl-N-nitrosourea (N-nitrosoethylurea) (ENU)
 Intestine - Large Intestine - Colon

21 - 25