Reference SummaryWasan HS, Proc Natl Acad Sci U S A 1997 Apr 1;94(7):3308-13

Title

Dietary fat influences on polyp phenotype in multiple intestinal neoplasia mice.

Authors

Wasan HS; Novelli M; Bee J; Bodmer WF

Journal

Proc Natl Acad Sci U S A

Volume

Issue

Year

1997

Pages

3308-13

Abstract

Significant differences in colon cancer incidence worldwide have led to the hypothesis that this variation can be explained largely by environmental, notably dietary influences. Although a positive correlation between dietary fat intake and incidence is suggested from some human epidemiological and rodent carcinogenesis studies, a direct association remains contentious. Using a spontaneous mouse tumor model of multiple intestinal neoplasia, we demonstrate that there is a generalized increase in tumor counts, in both the large and small bowel with higher dietary fat [standard (3%) fat versus high (15%) fat diet (mean +/- SD) 1.59 +/- 1.46 vs. 3.85 +/- 2.37 P < 0.001 and 21.36 +/- 7.4 vs. 31.3 +/- 9.7, respectively, P < 0.001]. Increasing dietary fat also increases polyp size in the small bowel. These changes appear independent of total calorific intake as assessed by body weights. Halving the crude fiber intake together with an increase in dietary fat from 3% to 10% did not have as marked an effect on tumor counts as an increase of fat alone to 15%, which also decreased survival (P < 0.05). These results demonstrate that increasing dietary fat intake from weaning can have a significant adverse effect on polyp numbers in mice genetically predisposed to intestinal tumor development. A further understanding of the biology of this interaction may provide novel strategies aimed at both colonic polyp prevention and treatment.

Links

J:39278 – MGI References
9096389 – National Library of Medicine/PubMed

Strain Notes

Strain	Note
C57BL/6J-Apc^Min/+	Mice were a gift from Dr. A. Moser. These mice were from Dr. A. Moser.

Models
Strain	Model Name	Treatment Agent(s)	Organ Affected	Frequency
C57BL/6J-Apc^Min/+	Intestine - Large Intestine polyp		Intestine - Large Intestine	observed
C57BL/6J-Apc^Min/+	Intestine - Large Intestine polyp	increased fat, decreased fiber	Intestine - Large Intestine	observed
C57BL/6J-Apc^Min/+	Intestine - Large Intestine polyp	high fat	Intestine - Large Intestine	observed
C57BL/6J-Apc^Min/+	Intestine - Small Intestine polyp		Intestine - Small Intestine	observed
C57BL/6J-Apc^Min/+	Intestine - Small Intestine polyp	increased fat, decreased fiber	Intestine - Small Intestine	observed
C57BL/6J-Apc^Min/+	Intestine - Small Intestine polyp	high fat	Intestine - Small Intestine	observed
C57BL/6J	Intestine tumor		Intestine	0
C57BL/6J-Apc^Min/+	Mammary gland tumor		Mammary gland	41
C57BL/6J-Apc^Min/+	Mammary gland tumor	increased fat, decreased fiber	Mammary gland	20
C57BL/6J-Apc^Min/+	Mammary gland tumor	high fat	Mammary gland	26