Reference SummarySteffensen IL, Carcinogenesis 1997 May;18(5):1049-54

Title

 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine increases the numbers of tumors, cystic crypts and aberrant crypt foci in multiple intestinal neoplasia mice.

Authors

 Steffensen IL; Paulsen JE; Eide TJ; Alexander J

Journal

 Carcinogenesis

Volume

 18

Issue

 5

Year

 1997

Pages

 1049-54

Abstract

 The multiple intestinal neoplasia (Min) mice have a mutation in the murine adenomatous polyposis coli (Apc) gene rendering them highly susceptible to spontaneous intestinal adenoma formation, similar to the familial adenomatous polyposis (FAP) syndrome in humans. We studied whether the most abundant mutagenic heterocyclic amine isolated from cooked food, 2-amino-1-methyl- 6- phenylimidazo[4,5-b]pyridine (PhIP), could influence early intestinal neoplasia in C57BL/6J-Min/+ and C57BL/6J-+/+ (wild-type) mice of both sexes. PhIP was given in 4 weekly i.p. Injections of 50 mg/kg, Ten weeks after the start of the experiment, PhIP had significantly increased the numbers of small tumors and cystic crypts in the proximal section of the small intestine in male Min/+ mice, and the numbers of aberrant crypt foci (ACF) in the large intestines of both males and females. The effects of PhIP were more pronounced in male than in female Min/+ mice. In +/+ mice, no tumors or cystic crypts in the small intestine, and no tumors and only a very few ACF in the large intestine, were induced by PhIP. These results show that a substance frequently present in the human diet is able to enhance the neoplastic process induced by a genetic lesion, which is also commonly found both in inherited and sporadic colon carcinomas in humans.

Links

 J:40366 – MGI References
9163695 – National Library of Medicine/PubMed

Strain Notes

Strain Note
C57BL/6J-ApcMin/+ These mice were bred from mice from The Jackson Laboratory.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6J Intestine - Large Intestine tumor Intestine - Large Intestine

0
C57BL/6J Intestine - Large Intestine tumor
  • 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)
 Intestine - Large Intestine

0
C57BL/6J-ApcMin/+ Intestine - Large Intestine tumor Intestine - Large Intestine

43 - 100
C57BL/6J-ApcMin/+ Intestine - Large Intestine tumor
  • 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)
 Intestine - Large Intestine

14 - 50
C57BL/6J-ApcMin/+ Intestine - Small Intestine adenoma Intestine - Small Intestine

100
C57BL/6J-ApcMin/+ Intestine - Small Intestine adenoma
  • 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)
 Intestine - Small Intestine

100
C57BL/6J Intestine - Small Intestine tumor Intestine - Small Intestine

0
C57BL/6J Intestine - Small Intestine tumor
  • 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)
 Intestine - Small Intestine

0