Reference SummaryShimokado K, Jpn J Cancer Res 1998 Mar;89(3):269-77

Title	p53 gene mutation and loss of heterozygosity of chromosome 11 in methylcholanthrene-induced mouse sarcomas.
Authors	Shimokado K; Watanabe H; Sumii M; Miyagawa K; Kamiya K; Dohi K ; Niwa O
Journal	Jpn J Cancer Res
Volume	89
Issue	3
Year	1998
Pages	269-77
Abstract	Mutations of the p53 tumor suppressor gene are the most prevalent genetic alteration observed in a wide variety of human cancers, In this study we examined 63 methylcholanthrene (MCA)-induced sarcomas from C57BL/ 6NxC3H/HeN F-1 (BCF1) or C3H/HeNxC57BL/6N F-1 (CBF1) mice for p53 gene mutations and loss of heterozygosity (LOH) of chromosome 11, Mutation analysis was done on exons 5 to 8 of the p53 gene by polymerase chain reaction-single strand conformation polymorphism analysis, This identified 53 potential mutations in 45 sarcomas, Mutations were further confirmed by direct sequencing of the region, Forty-nine of the 53 cases (94%) were missense mutations, while the rest included two nonsense mutations, one silent mutation and one insertional mutation, Spectra of base substitutions were: 25 cases (47%) of G:C-->T:A transversion, 13 cases (25%) of G:C-->A:T transition (CpG site 15%), 13 cases (24%) of G:C-->C:G transversion, a case (2%) of A:T-->T:A transversion and a case (2%) of insertion, In addition, analysis of 5 polymorphic markers of mouse chromosome 11 revealed LOH in ten cases (22%) among those carrying p53 mutations, In nine of these 10 cases, the loss involved all 5 markers, In addition, the loss was biased toward the C57BL allele (9 cases), The present study establishes the pattern of mutation of the p53 gene in MCA-induced mouse sarcomas.
Links	J:47139 – MGI References 9600120 – National Library of Medicine/PubMed

Models
Strain	Model Name	Treatment Agent(s)	Organ Affected	Frequency	Model Details
(C3H/HeN x C57BL/6N)F1	Mesodermal cell/mesoblast sarcoma	methylcholanthrene (MCA)	Mesodermal cell/mesoblast	observed
(C57BL/6N x C3H/HeN)F1	Mesodermal cell/mesoblast sarcoma	methylcholanthrene (MCA)	Mesodermal cell/mesoblast	observed