Reference SummaryBashir O, Carcinogenesis 2004 Aug;25(8):1507-15

Title

 Both suboptimal and elevated vitamin intake increase intestinal neoplasia and alter crypt fission in the ApcMin/+ mouse.

Authors

 Bashir O; FitzGerald AJ; Goodlad RA

Journal

 Carcinogenesis

Volume

 25

Issue

 8

Year

 2004

Pages

 1507-15

Abstract

 The effects of vitamin deficiency on intestinal cancer are unclear, and even less is known about the consequences of excessive intake. We therefore investigated the actions of altered vitamin content on intestinal polyp development, cell proliferation and crypt fission in a mouse model of neoplasia. Ninety multiple intestinal neoplasia (Apc(Min/+)) mice and 90 wild-type littermates, 4 weeks old, were divided into six groups and fed either a control semi-synthetic diet, or the semi-synthetic diet with the vitamin content lowered to a third of the RDA or the semi-synthetic diet with the vitamin content increased 5-fold (except for retinol and folate, which were doubled). The number and size of polyps in the small and large intestines were scored after 8 weeks on the diets, as was cell proliferation (native mitoses per crypt) and crypt fission. The small intestines of the low and high vitamin groups were heavier than the controls. There were significantly more polyps and the tumour burden was higher in both the low and the high vitamin groups (P < 0.02). Proliferation was slightly reduced by the vitamin alteration and crypt fission was significantly increased in the Apc(Min/+) mice when compared with the wild-type (P < 0.001). Fission indices were decreased by vitamin alteration in the small intestine, and increased in the colon, but only in the Apc(Min/+) mice. The effects of vitamin alteration on polyp number were most pronounced in the proximal intestine, which is also the site of maximum crypt fission. Both vitamin deficiency and over-supplementation can markedly enhance polyp number and tumour burden.

Links

 J:91525 – MGI References
15016659 – National Library of Medicine/PubMed

Strain Notes

Strain Note
C57BL/6J Mice were produced in house and were used as controls for C57BL/6J-ApcMin/+ mice that were originally obtained from Dr. A. Moser.
C57BL/6J-ApcMin/+ Original C57BL/6-ApcMin/+ mice were obtained from Dr. Amy Moser, and bred inhouse to C57BL/6J females by brother/sister mating.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C57BL/6J Intestine - Large Intestine - Colon polyp Intestine - Large Intestine - Colon

0
C57BL/6J Intestine - Large Intestine - Colon polyp
  • low vitamin
 Intestine - Large Intestine - Colon

0
C57BL/6J Intestine - Large Intestine - Colon polyp
  • high vitamin
 Intestine - Large Intestine - Colon

0
C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon polyp Intestine - Large Intestine - Colon

observed
C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon polyp
  • low vitamin
 Intestine - Large Intestine - Colon

observed
C57BL/6J-ApcMin/+ Intestine - Large Intestine - Colon polyp
  • high vitamin
 Intestine - Large Intestine - Colon

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Distal polyp Intestine - Small Intestine - Distal

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Distal polyp
  • low vitamin
 Intestine - Small Intestine - Distal

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Distal polyp
  • high vitamin
 Intestine - Small Intestine - Distal

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Medial polyp Intestine - Small Intestine - Medial

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Medial polyp
  • low vitamin
 Intestine - Small Intestine - Medial

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Medial polyp
  • high vitamin
 Intestine - Small Intestine - Medial

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Proximal polyp Intestine - Small Intestine - Proximal

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Proximal polyp
  • low vitamin
 Intestine - Small Intestine - Proximal

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine - Proximal polyp
  • high vitamin
 Intestine - Small Intestine - Proximal

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine polyp Intestine - Small Intestine

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine polyp
  • low vitamin
 Intestine - Small Intestine

observed
C57BL/6J-ApcMin/+ Intestine - Small Intestine polyp
  • high vitamin
 Intestine - Small Intestine

observed
C57BL/6J Intestine - Small Intestine polyp Intestine - Small Intestine

0
C57BL/6J Intestine - Small Intestine polyp
  • low vitamin
 Intestine - Small Intestine

0
C57BL/6J Intestine - Small Intestine polyp
  • high vitamin
 Intestine - Small Intestine

0