Pathology Image Detail

Caption	SupFig. S4G lumbar lymph node metastasis from an NPK tumor-bearing mouse
Description	lymph node metastases from prostate tumor; whole slide scan, H&E, and immunohistochemistry for various markers
Notes	Suppl. Fig. S4C-K
Contributor	Aytes A (J:200917)
Pathologist	Aytes A (J:200917)
Copyright	This image is from Aytes A, Proc Natl Acad Sci U S A 2013;110(37):E3506-15. Copyright 2013 National Academy of Sciences, U.S.A.
Method	IHC for androgen receptor

Image Probes
Type: antibody	Name anti-AR (N-20) (sc-816) Target androgen receptor (AR) [mouse, rat, and human] Supplier Santa Cruz Biotechnology Notes "Affinity purified rabbit polyclonal antibody raised against a peptide mapping at the amino terminus of the androgen receptor of human origin (identical to the corresponding mouse sequence)."

Model

MMHC ID: MTB:60975

Tumor Name: Prostate gland • adenocarcinoma

Treatment Type: Genetic Modification

Agent: [Agent] - Agent._Agent_key = [137] - Agent._AgentType_key = [14] - Agent.name = [tamoxifen (for inducing expression from mutant alleles)] - Agent.description = [null] - Agent.create_user = [MTB] - Agent.create_date = [2005-01-05 13:31:30.0] - Agent.update_user = [dmk] - Agent.update_date = [2024-03-11 10:17:27.528]

Tumor Synonyms: prostate carcinoma; prostate adenocarcinoma; prostate tumor

Organ Affected: Lymph node

Frequency: observed

Frequency Note: Beginning at 2 months of age mice were given tamoxifen (100 mg/kg in corn oil) by oral gavage once daily for 4 days.

Reference: J:200917

Strain

Strain: STOCK Kras^tm4Tyj/+ Nkx3-1^{tm4(cre/ERT2)Mms}/+ Pten^tm1Hwu

Strain Types: targeted mutation (conditional); targeted mutation (knock-in)

General Note: Compound mutant mice. This STOCK genetic background included C57BL/6, "129/Sv" (including 129S6/SvEvTac and 129S4/SvJae), and FVB.

Strain Synonyms: Nkx3.1^CreERT2/+; Pten^flox/flox; Kras^12D/+; NPK; Nkx3.1^CreERT2/+; Pten^flox/flox; Kras^LSL-G12D/+; Nkx3.1^CreERT2/+; Pten^flox/flox; Kras^G12D/+; Nkx3.1^CE2/+; Pten^f/f; Kras^12D/+

Strain Sex: Male

Reproductive Status: reproductive status not specified

Age of Detection: 4-7 months