Pathology Image Detail

Caption	Blood vessel - normal tissue. Aorta: there is strong cytoplasmic immunolabeling of endothelial cells of the aorta for platelet-derived growth factor alpha (antibody diluted at 1:25, tissue fixed in IHC Zinc fixative, heat-mediated antigen retrieval in citrate buffer at pH=6.0). There is also mild multifocal staining of the connective tissue in the wall of the aorta. This pattern of immunolabeling was consistently detected with this antibody but it may represent an artifact.
Description	Blood vessel - Normal tissue. Platelet-derived growth factor alpha, endothelial cells of aorta.
Age at Necropsy	35 days
Contributor	Mikaelian I (J:94320)
Pathologist	Mikaelian I (J:94320)
Method	IHC for platelet-derived growth factor alpha (PDGFA)

Image Probes
Type: antibody	Name anti-PDGF-A (E-10) (sc-9974) Target platelet-derived growth factor alpha (PDGFA) Supplier Santa Cruz Biotechnology Notes Mouse monoclonal IgG2b raised against amino acids 135-211 mapping at the C-terminus of PDGFA of human origin.

Strain Synonyms: wild-type C57BL/6J; C57Bl/6J; p53+/+; C57BL/6 from Jackson Laboratory; C57BL/6; B6; C57/Bl6; C57; C57BL/6J (F₁₂₉₊₅₁); C57 black subline 6; B/6; Bl/6; C57BL/6J wild-type; WT; Black 6; C57 Black; wild type; C57BL/6J^+/+; p53 wild-type (+/+); C57BL6/J; C57BL/6J, H-2^b; C57/B16; wild-type; C57BL; B6 WT; wild-type (WT); WT-Control; WT-CR; C57/Bl 6J; C57/Bl; p21+/+p27+/+; control; Ctr; C57BL/6J (UW); B6J; C57BL/6J inbred WT; C57 BL/6; Wild-type (WT) C57BL/6J