Pathology Image Detail

Caption

Uterus - normal tissue. There is moderate labeling of the cytoplasm of smooth muscle fibers of the outer portions of the wall of the uterus for vimentin (antibody diluted at 1:500, tissue fixed in Fekete's solution, heat-mediated antigen retrieval in citrate buffer at pH=6.0). There is weak labeling of the cytoplasm of smooth muscle fibers of the inner portions of the wall of the uterus for vimentin.
NOTE: This antibody against vimentin fails to label most skeletal muscle fibers. In addition, it labels some epithelia. It is also associated with some background and small clumps of brown pigment scattered all over the slide. Centrifugation of the antibody did not prevent the formation of small clumps of brown pigment over the tissues.

Description

Uterus - Normal tissue. Vimentin immunohistochemistry.

Age at Necropsy

35 days

Contributor

Mikaelian I (J:94320)

Pathologist

Mikaelian I (J:94320)

Method

IHC for vimentin
Image Probes

Type: antibody

Name
anti-vimentin
Target
human vimentin (VIM)
Supplier
Biomeda Corporation
Notes
polyclonal rabbit anti-human vimentin

Model

MMHC ID
MTB:27714
Tumor Name
Uterus • normal tissue (control)
Treatment Type
None (spontaneous)
Tumor Synonyms
uterus, normal tissue
Organ Affected
Uterus
Frequency
not applicable
Reference
J:94320

Strain

Strain
C57BL/6J
Strain Types
inbred
Strain Synonyms
wild-type C57BL/6J
C57Bl/6J
p53+/+
C57BL/6 from Jackson Laboratory
C57BL/6
B6
C57/Bl6
C57
C57BL/6J (F129+51)
C57 black subline 6
B/6
Bl/6
C57BL/6J wild-type
WT
Black 6
C57 Black
wild type
C57BL/6J+/+
p53 wild-type (+/+)
C57BL6/J
C57BL/6J, H-2b
C57/B16
wild-type
C57BL
B6 WT
wild-type (WT)
WT-Control
WT-CR
C57/Bl 6J
C57/Bl
p21+/+p27+/+
control
Ctr
C57BL/6J (UW)
B6J
C57BL/6J inbred WT
C57 BL/6
Wild-type (WT) C57BL/6J
Strain Sex
Female
Reproductive Status
reproductive status not specified
Age of Detection
35 days