Caption |
Uterus - normal tissue. There is moderate labeling of the cytoplasm of smooth muscle fibers of the outer portions of the wall of the uterus for vimentin (antibody diluted at 1:500, tissue fixed in Fekete's solution, heat-mediated antigen retrieval in citrate buffer at pH=6.0). There is weak labeling of the cytoplasm of smooth muscle fibers of the inner portions of the wall of the uterus for vimentin.
NOTE: This antibody against vimentin fails to label most skeletal muscle fibers. In addition, it labels some epithelia. It is also associated with some background and small clumps of brown pigment scattered all over the slide. Centrifugation of the antibody did not prevent the formation of small clumps of brown pigment over the tissues. |