Pathology Image Detail

Caption	Haired skin - normal tissue. A cross section of a hair follicle shows strong labeling of the inner layer of the outer root sheath for keratins 5/8 (antibody diluted at 1:100, tissue fixed in Fekete's solution, enzymatic digestion in trypsin). Keratin 5 is a marker of basal cells while keratin 8 is a marker of simple epithelia. Hence labeling in this case is interpreted as the result of a cross-reaction of the antibody with undetermined proteins. NOTE: Haired skin, esophageal mucosa, and oral mucosa can be used as positive control for this antibody. However, this antibody does not label all the structures it is supposed to label based on reported expression patterns for keratins 5 and 8.
Description	Haired skin - Normal tissue. Keratins 5 and 8 immunohistochemistry, non-specific labeling
Age at Necropsy	35 days
Contributor	Mikaelian I (J:94320)
Pathologist	Mikaelian I (J:94320)
Method	IHC for keratin 5 and 8

Image Probes
Type: antibody	Name anti-keratin 5 and 8 (MAB1629) Target human keratin 5 and 8 Supplier Chemicon Notes Specificity: Human keratins 5 (58 kD) and 8 (52.5 kD). MAB1629 cross-reacts with many mammalian species. Negative on chicken and Xenopus.

Strain Synonyms: wild-type C57BL/6J; C57Bl/6J; p53+/+; C57BL/6 from Jackson Laboratory; C57BL/6; B6; C57/Bl6; C57; C57BL/6J (F₁₂₉₊₅₁); C57 black subline 6; B/6; Bl/6; C57BL/6J wild-type; WT; Black 6; C57 Black; wild type; C57BL/6J^+/+; p53 wild-type (+/+); C57BL6/J; C57BL/6J, H-2^b; C57/B16; wild-type; C57BL; B6 WT; wild-type (WT); WT-Control; WT-CR; C57/Bl 6J; C57/Bl; p21+/+p27+/+; control; Ctr; C57BL/6J (UW); B6J; C57BL/6J inbred WT; C57 BL/6; Wild-type (WT) C57BL/6J