Caption |
Haired skin - normal tissue. Scattered dentritic cells located in the epidermis stain strongly for vimentin (antibody diluted at 1:1,000, tissue fixed in Bouin's solution, heat-mediated antigen retrieval in citrate buffer at pH=6.0). These cells are interpreted as being melanocytes.
NOTE: This antibody against vimentin fails to label most skeletal muscle fibers. In addition, it labels some epithelia. It is also associated with some background and small clumps of brown pigment scattered all over the slide. Centrifugation of the antibody did not prevent the formation of small clumps of brown pigment over the tissues. |