Caption |
Colon - normal tissue. There is moderate labeling of smooth muscle fibers of the intestinal wall. Myenteric plexuses and endothelial cells are labeled, too. Epithelial cells are not labeled (antibody diluted at 1:1,000, tissue fixed in Bouin's solution, heat-mediated antigen retrieval in citrate buffer at pH=6.0).
NOTE: This antibody against vimentin fails to label most skeletal muscle fibers. In addition, it labels some epithelia. It is also associated with some background and small clumps of brown pigment scattered all over the slide. Centrifugation of the antibody did not prevent the formation of small clumps of brown pigment over the tissues. |