Caption |
Colon - normal tissue. The cytoplasm of cells from myenteric plexuses shows strong immunolabeling for vimentin. Smooth muscle fibers of the wall of the colon show weak cytoplasmic labeling (antibody diluted at 1:500, tissue fixed in Fekete's solution, heat-mediated antigen retrieval in citrate buffer at pH=6.0). Labeling of smooth muscle fibers is weaker for tissues fixed in Fekete's solution than in Bouin's solution.
NOTE: This antibody against vimentin fails to label most skeletal muscle fibers. In addition, it labels some epithelia. It is also associated with some background and small clumps of brown pigment scattered all over the slide. Centrifugation of the antibody did not prevent the formation of small clumps of brown pigment over the tissues. |
