Pathology Image Detail

Caption

Duodenum - normal tissue. There is strong nuclear immunolabeling for PCNA of epithelial cells of intestinal crypts (antibody diluted at 1:100, tissue fixed in Fekete's solution). There is mild cytoplasmic background in smooth muscle fibers of the wall of the intestine as well as in epithelial cells of the intestine.

Description

Duodenum - normal tissue. Proliferating cell nuclear antigen immunohistochemistry

Age at Necropsy

35 days

Contributor

Mikaelian I (J:94320)

Pathologist

Mikaelian I (J:94320)

Method

IHC for proliferating cell nuclear antigen (PCNA)
Image Probes

Type: antibody

Name
anti-PCNA (PC10) (sc-56)
Target
proliferating cell nuclear antigen (PCNA) p36 protein [mouse, rat, human, insect, & S. pombe]
Supplier
Santa Cruz Biotechnology
Notes
"Mouse monoclonal IgG2a antibody derived by fusion of spleen cells from a BALB/c mouse with recombinant PCNA with Sp2/0-Ag14 myeloma cells."

Model

MMHC ID
MTB:27657
Tumor Name
Intestine - Small Intestine - Duodenum • normal tissue (control)
Treatment Type
None (spontaneous)
Tumor Synonyms
duodenum, normal tissue
Organ Affected
Intestine - Small Intestine - Duodenum
Frequency
not applicable
Reference
J:94320

Strain

Strain
C57BL/6J
Strain Types
inbred
Strain Synonyms
wild-type C57BL/6J
C57Bl/6J
p53+/+
C57BL/6 from Jackson Laboratory
C57BL/6
B6
C57/Bl6
C57
C57BL/6J (F129+51)
C57 black subline 6
B/6
Bl/6
C57BL/6J wild-type
WT
Black 6
C57 Black
wild type
C57BL/6J+/+
p53 wild-type (+/+)
C57BL6/J
C57BL/6J, H-2b
C57/B16
wild-type
C57BL
B6 WT
wild-type (WT)
WT-Control
WT-CR
C57/Bl 6J
C57/Bl
p21+/+p27+/+
control
Ctr
C57BL/6J (UW)
B6J
C57BL/6J inbred WT
C57 BL/6
Wild-type (WT) C57BL/6J
Strain Sex
Unspecified
Reproductive Status
reproductive status not specified
Age of Detection
35 days