Spleen - normal tissue. There is strong membrane and cytoplasmic labeling for CD45/B220 of lymphocytes in lymphoid follicles and periarteriolar lymphoid sheaths (antibody diluted at 1:250, tissue fixed in Fekete's solution).
Spleen - Normal tissue. CD45/B220 immunohistochemistry
"Specifically recognizes the exon B-dependent isoform of mouse CD45, which is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density. This isoform is also expressed by dendritic cells and macrophages at low density."