Caption |
Spleen - normal tissue. There is strong cytoplasmic and plasma membrane labeling of scattered round to dendritic cells located in the red pulp (tissue fixed in 4% paraformaldehyde, antibody diluted at 1:100, heat-mediated antigen retrieval in citrate buffer).
NOTE: This pattern of labeling was interpreted as an artifact because L-selectin is normally expressed on peripheral lymphocytes, NK cells, neutrophils, eosinophils, and monocytes while only cells with a monocytic or dendritic phenotype labeled with this antibody. |