Caption |
Spleen - normal tissue. There is strong labeling of the plasma membrane and of the cytoplasm of scattered round to dendritic cells of the red pulp (tissue fixed in 4% paraformaldehyde, antibody diluted at 1:25, heat-mediated antigen retrieval in citrate buffer).
NOTE: This pattern of labeling was interpreted as an artifact because L-selectin is normally expressed on peripheral lymphocytes, NK cells, neutrophils, eosinophils, and monocytes while only cells with a monocytic or dendritic phenotype labeled with this antibody. |