Pathology Image Detail

Caption	Spleen - normal tissue. There is strong labeling of the cytoplasm of a megakaryocyte for P-selectin (antibody diluted at 1:25, tissue fixed in Fekete's solution, heat-mediated antigen retrieval in citrate buffer at pH=6.0). Platelets are not labeled (platelets, when labeled, appear as minute brown dots located in the sinuses of the red pulp and in blood vessels). Platelets are labeled when the antigen retrieval solution is EDTA instead of citrate buffer.
Description	Spleen - Normal tissue. P-selectin immunohistochemistry
Age at Necropsy	35 days
Contributor	Mikaelian I (J:94320)
Pathologist	Mikaelian I (J:94320)
Method	IHC for P-selectin

Image Probes
Type: antibody	Name anti-mouse P-selectin (CD62P) (CBL40) Target selectin, platelet (Selp) [P-selectin] Supplier Chemicon Notes rabbit anti-mouse polyclonal antibody

Strain Synonyms: wild-type C57BL/6J; C57Bl/6J; p53+/+; C57BL/6 from Jackson Laboratory; C57BL/6; B6; C57/Bl6; C57; C57BL/6J (F₁₂₉₊₅₁); C57 black subline 6; B/6; Bl/6; C57BL/6J wild-type; WT; Black 6; C57 Black; wild type; C57BL/6J^+/+; p53 wild-type (+/+); C57BL6/J; C57BL/6J, H-2^b; C57/B16; wild-type; C57BL; B6 WT; wild-type (WT); WT-Control; WT-CR; C57/Bl 6J; C57/Bl; p21+/+p27+/+; control; Ctr; C57BL/6J (UW); B6J; C57BL/6J inbred WT; C57 BL/6; Wild-type (WT) C57BL/6J