Caption |
Tongue - normal tissue. There is moderate labeling of the cytoplasm of skeletal muscles and suprabasal epithelial cells for vimentin (antibody diluted at 1:500, tissue fixed in Fekete's solution, heat-mediated antigen retrieval in citrate buffer at pH=6.0). There is strong labeling of submucosal fibrocytes for vimentin. Labeling of suprabasal epithelial cells of the oral mucosa is interpreted as an artifact.
NOTE: This antibody against vimentin fails to label most skeletal muscle fibers. In addition, it labels some epithelia. It is also associated with some background and small clumps of brown pigment scattered all over the slide. Centrifugation of the antibody did not prevent the formation of small clumps of brown pigment over the tissues. |