Reference SummaryCannon RE, Mol Carcinog 1997 Sep;20(1):108-14

Title

 Kinetics of wound-induced v-Ha-ras transgene expression and papilloma development in transgenic Tg.AC mice.

Authors

 Cannon RE; Spalding JW; Trempus CS; Szczesniak CJ; Virgil KM; Humble MC; Tennant RW

Journal

 Mol Carcinog

Volume

 20

Issue

 1

Year

 1997

Pages

 108-14

Abstract

 The Tg.AC transgenic mouse, which harbors an activated v-Ha-ras coding region that is fused to an embryonic zeta globin transcriptional control region and a 3' simian virus 40 polyadenylation sequence, rapidly develops epidermal papillomas in response to topical application of chemical carcinogens or tumor promoters or to full-thickness wounding of the dorsal skin. In this report, we investigated the localization and temporal induction of v-Ha-ras transgene expression after full-thickness wounding of Tg.AC mouse skin. Surgically inflicted full-thickness incisions 3 cm long yielded four to six papillomas per Tg.AC mouse by 5 wk after wounding. Similar wounding of the FVB/N isogenic host strain did not produce tumors, which implicates a causal role for the v-Ha-ras transgene. Reverse transcription-polymerase chain reaction assays detected the v-Ha-ras transgene transcript in total RNA samples isolated from wound-associated tissue 3 and 4 wk after wounding. Tissues 1-2 wk after wounding and all non-wound-associated tissues were negative for transgene expression. In situ hybridization experiments using transgene-specific 35S-labeled antisense RNA probes localized transgene expression to the basal epidermal cells in wound-induced papillomas. Adjacent normal and hyperplastic skin tissues were negative for transgene expression by this assay. This work supports the hypothesis that the wound repair response leads to the transcriptional activation and continued expression of the v-Ha-ras transgene in specific cells in the skin, which alters normal epithelial differentiation and ultimately results in neoplastic growth.

Links

 J:44372 – MGI References
9328441 – National Library of Medicine/PubMed

Strain Notes

Strain Note
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed These mice were purchased from Taconic.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed Skin hyperplasia
  • wounding
 Skin

observed
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed Skin papilloma
  • wounding
 Skin

100
FVB/NTac Skin tumor
  • wounding
 Skin

0