Reference SummaryTrempus CS, J Invest Dermatol 1998 Sep;111(3):445-51

Title

 Photocarcinogenesis and susceptibility to UV radiation in the v-Ha-ras transgenic Tg.AC mouse.

Authors

 Trempus CS; Mahler JF; Ananthaswamy HN; Loughlin SM; French JE; Tennant RW

Journal

 J Invest Dermatol

Volume

 111

Issue

 3

Year

 1998

Pages

 445-51

Abstract

 The v-Ha-ras transgenic Tg.AC mouse line has proven to be a useful model for the study of chemical carcinogenic potential. We undertook experiments designed to study the effect of the physical carcinogen, UV radiation, on tumorigenesis in this mouse strain. Following a total of three exposures on alternating days to a radiation source covering a cumulative UVR exposure range of 2.6-42.6 kJ per m2, squamous papillomas developed by 4 wk after initial exposure in a dose-dependent manner. Malignancies developed within 18-30 wk following the initial UVR exposure and were all diagnosed as squamous cell carcinoma or spindle cell tumors. In contrast to other mouse stains used in photocarcinogenesis studies, few p53 mutations were found in Tg.AC malignancies upon polymerase chain reaction-single stranded conformational polymorphism analysis of exons 4-8 followed by sequencing of suspicious bands; however, all tumors analyzed by in situ hybridization expressed the v-Ha-ras transgene. Immunohistochemical analysis of UVR-exposed skin taken 24 h after the last of three exposures (13.1 kJ per m2 total UVR) showed expression of p53 in hair follicles and in interfollicular epidermis, which indicates that the gene was functional. Thus, although there are some differences between the Tg.AC and other mouse models, these results suggest that the Tg.AC mouse may be a useful model for the study of acute exposure photocarcinogenesis.

Links

 J:49862 – MGI References
9740239 – National Library of Medicine/PubMed

Strain Notes

Strain Note
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed These mice were purchased from Taconic.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB/NTac Skin papilloma
  • ultraviolet radiation (UV)
 Skin - Dorsal region

0 - 10
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed Skin papilloma
  • ultraviolet radiation (UV)
 Skin - Dorsal region

0 - 100
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed Skin squamous cell carcinoma
  • ultraviolet radiation (UV)
 Skin - Dorsal region

observed - 60
FVB/NTac-Tg(Hba-x-v-Ha-ras)TG.ACLed (Unspecified organ) tumor - spindle cell
  • ultraviolet radiation (UV)
 Skin - Dorsal region

observed - 20