Reference SummaryTamura T, Cancer Lett 1999 Aug 23;143(1):71-80

Title

 Tumor-promoting activities of hydroquinone and 1,1-dimethylhydrazine after initiation of newborn mice with 1-methyl-1-nitrosourea.

Authors

 Tamura T; Shibutani M; Toyoda K; Shoda T; Takada K; Uneyama C; Takahashi M; Hirose M

Journal

 Cancer Lett

Volume

 143

Issue

 1

Year

 1999

Pages

 71-80

Abstract

 To clarify the suitability of a newborn-mouse carcinogenesis assay to detect tumor-promoting activities of carcinogens, the non-genotoxic hydroquinone (HQ) and genotoxic 1,1-dimethylhydrazine (UDMH) were administered to mice during the promotion stage after treatment with 1-methyl-1-nitrosourea (MNU) (20 mg/kg body wt, single intraperitoneal injection) at day 9 after birth. Initiated males and females thus received either HQ at 0.8% in basal diet, or UDMH, at 20 mg/kg body wt once weekly by subcutaneous injection, from day 14 until the end of the experiment at 30 weeks of age. Uninitiated newborn mice, given an injection of the vehicle (0.01 M citrate buffer (pH 5.5), 20 mg/kg body wt), also received HQ or UDMH in the same way. Histopathologically, focal proliferative lesions were found in the livers of male mice and in the lungs of both male and female mice in the MNU-treated groups. HQ significantly increased the incidence and multiplicity of altered hepatocellular foci, the combined incidence of hepatocellular adenomas and carcinomas in males and the incidence and multiplicity of lung adenomas and the combined incidence of lung adenomas and carcinomas in female mice. In addition, four out of eleven MNU + HQ-treated male mice developed lung carcinomas, showing a significant elevation in multiplicity. UDMH also exhibited a tendency to increase the incidence and multiplicity of lung adenomas in female mice. Thus tumor-promoting effects of HQ or UDMH were apparently exerted in the target organs and the MNU-initiated two-stage newborn-mouse carcinogenesis assay may be useful for detection of genotoxic or non-genotoxic carcinogenicity.

Links

 J:57141 – MGI References
10465340 – National Library of Medicine/PubMed

Strain Notes

Strain Note
CD-1 These mice were purchased from Charles River Japan.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
CD-1 Leukocyte lymphoma
  • 1-methyl-1-nitrosourea (MNU)
 Leukocyte

10
CD-1 Liver - Hepatocyte preneoplastic lesion
  • 1-methyl-1-nitrosourea (MNU)
 Liver - Hepatocyte

0 - 40
CD-1 Liver - Hepatocyte preneoplastic lesion
  • 1-methyl-1-nitrosourea (MNU)
  • hydroquinone (HQ)
 Liver - Hepatocyte

13 - 91
CD-1 Liver - Hepatocyte preneoplastic lesion
  • 1-methyl-1-nitrosourea (MNU)
  • 1,1-dimethylhydrazine (UDMH)
 Liver - Hepatocyte

0 - 40
CD-1 Liver - Hepatocyte preneoplastic lesion
  • hydroquinone (HQ)
 Liver - Hepatocyte

0
CD-1 Liver - Hepatocyte preneoplastic lesion
  • 1,1-dimethylhydrazine (UDMH)
 Liver - Hepatocyte

0
CD-1 Liver hepatocellular adenoma
  • 1-methyl-1-nitrosourea (MNU)
 Liver

0 - 10
CD-1 Liver hepatocellular adenoma
  • 1-methyl-1-nitrosourea (MNU)
  • hydroquinone (HQ)
 Liver

0 - 45
CD-1 Liver hepatocellular adenoma
  • 1-methyl-1-nitrosourea (MNU)
  • 1,1-dimethylhydrazine (UDMH)
 Liver

0 - 13
CD-1 Liver hepatocellular adenoma
  • hydroquinone (HQ)
 Liver

0
CD-1 Liver hepatocellular adenoma
  • 1,1-dimethylhydrazine (UDMH)
 Liver

0
CD-1 Liver hepatocellular carcinoma
  • 1-methyl-1-nitrosourea (MNU)
 Liver

0
CD-1 Liver hepatocellular carcinoma
  • 1-methyl-1-nitrosourea (MNU)
  • hydroquinone (HQ)
 Liver

0 - 9.1
CD-1 Liver hepatocellular carcinoma
  • 1-methyl-1-nitrosourea (MNU)
  • 1,1-dimethylhydrazine (UDMH)
 Liver

0 - 6.7
CD-1 Liver hepatocellular carcinoma
  • hydroquinone (HQ)
 Liver

0
CD-1 Liver hepatocellular carcinoma
  • 1,1-dimethylhydrazine (UDMH)
 Liver

0
CD-1 Lung - Alveolus hyperplasia
  • 1-methyl-1-nitrosourea (MNU)
 Lung - Alveolus

9.1 - 20
CD-1 Lung - Alveolus hyperplasia
  • 1-methyl-1-nitrosourea (MNU)
  • hydroquinone (HQ)
 Lung - Alveolus

9.1 - 13
CD-1 Lung - Alveolus hyperplasia
  • 1-methyl-1-nitrosourea (MNU)
  • 1,1-dimethylhydrazine (UDMH)
 Lung - Alveolus

0
CD-1 Lung - Alveolus hyperplasia
  • hydroquinone (HQ)
 Lung - Alveolus

0
CD-1 Lung - Alveolus hyperplasia
  • 1,1-dimethylhydrazine (UDMH)
 Lung - Alveolus

0
CD-1 Lung adenoma
  • 1-methyl-1-nitrosourea (MNU)
 Lung

27 - 80
CD-1 Lung adenoma
  • 1-methyl-1-nitrosourea (MNU)
  • hydroquinone (HQ)
 Lung

55 - 87
CD-1 Lung adenoma
  • 1-methyl-1-nitrosourea (MNU)
  • 1,1-dimethylhydrazine (UDMH)
 Lung

47 - 62.5
CD-1 Lung adenoma
  • hydroquinone (HQ)
 Lung

0 - 6.7
CD-1 Lung adenoma
  • 1,1-dimethylhydrazine (UDMH)
 Lung

0
CD-1 Lung carcinoma
  • 1-methyl-1-nitrosourea (MNU)
 Lung

0 - 9.1
CD-1 Lung carcinoma
  • 1-methyl-1-nitrosourea (MNU)
  • hydroquinone (HQ)
 Lung

6.7 - 36
CD-1 Lung carcinoma
  • 1-methyl-1-nitrosourea (MNU)
  • 1,1-dimethylhydrazine (UDMH)
 Lung

6.3 - 20
CD-1 Lung carcinoma
  • hydroquinone (HQ)
 Lung

0
CD-1 Lung carcinoma
  • 1,1-dimethylhydrazine (UDMH)
 Lung

0
CD-1 Nose - Nasal cavity adenoma
  • 1-methyl-1-nitrosourea (MNU)
 Nose - Nasal cavity

10
CD-1 Nose - Nasal cavity adenoma
  • 1-methyl-1-nitrosourea (MNU)
  • 1,1-dimethylhydrazine (UDMH)
 Nose - Nasal cavity

6.7