Reference SummaryAhn B, Cancer Res 2001 Dec 1;61(23):8357-60

Title

Suppression of intestinal polyposis in Apc(Min/+) mice by inhibiting nitric oxide production.

Authors

Ahn B; Ohshima H

Journal

Cancer Res

Volume

Issue

Year

2001

Pages

8357-60

Abstract

Inducible nitric oxide synthase (iNOS) was shown to be expressed in normal mucosa and adenoma of small and large intestines of Apc(Min/+) mice by reverse transcription-PCR and immunohistochemistry. Administration of the iNOS inhibitor aminoguanidine (1.5 g/liter) in drinking water or an L-arginine-deficient diet to Apc(Min/+) mice resulted in a significant decrease in adenoma development in the small but not the large intestine. Similarly, iNOS-gene knockout Apc(Min/+) mice (Apc(Min/+) iNOS(-/-) or Apc(Min/+) iNOS(-/+)) developed significantly fewer adenomas in both small and large intestines than Apc(Min/+) iNOS(+/+) mice. These results suggest that iNOS-selective inhibitors could be used as a potential chemopreventive agent for colorectal cancers.

Links

J:73152 – MGI References
11731407 – National Library of Medicine/PubMed

Strain Notes

Strain	Note
B6;129P2-Apc^Min/+	Both Apc and Nos2 mutant mice were purchased at 6 weeks of age from The Jackson Laboratory. Male Apc^Min/+ mice may have been bred to wild type C57BL/6Icr females prior to mating with Nos2 knockout mice.
B6;129P2-Apc^Min/+ Nos2^tm1Lau	Both Apc and Nos2 mutant mice were purchased at 6 weeks of age from The Jackson Laboratory. Male Apc^Min/+ mice may have been bred to wild type C57BL/6Icr females prior to mating with Nos2 knockout mice.
B6;129P2-Apc^Min/+ Nos2^tm1Lau/+	Both Apc and Nos2 mutant mice were purchased at 6 weeks of age from The Jackson Laboratory. Male Apc^Min/+ mice may have been bred to wild type C57BL/6Icr females prior to mating with Nos2 knockout mice.
C57BL/6-Apc^Min/+	C57BL/6J-Apc^Min/+ males purchased from The Jackson Laboratory were mated with C57BL/6Icr females.

Models
Strain	Model Name	Treatment Agent(s)	Organ Affected	Frequency
C57BL/6-Apc^Min/+	Intestine - Large Intestine adenoma		Intestine - Large Intestine	22 - 33
C57BL/6-Apc^Min/+	Intestine - Large Intestine adenoma	aminoguanidine hemisulfate	Intestine - Large Intestine	50
C57BL/6-Apc^Min/+	Intestine - Large Intestine adenoma	L-arginine-deficient (Harlan Teklad TD 91230)	Intestine - Large Intestine	33
B6;129P2-Apc^Min/+ Nos2^tm1Lau	Intestine - Large Intestine adenoma		Intestine - Large Intestine	29
B6;129P2-Apc^Min/+ Nos2^tm1Lau/+	Intestine - Large Intestine adenoma		Intestine - Large Intestine	68
B6;129P2-Apc^Min/+	Intestine - Large Intestine adenoma		Intestine - Large Intestine	100
C57BL/6-Apc^Min/+	Intestine - Small Intestine adenoma		Intestine - Small Intestine	observed
C57BL/6-Apc^Min/+	Intestine - Small Intestine adenoma	aminoguanidine hemisulfate	Intestine - Small Intestine	observed
C57BL/6-Apc^Min/+	Intestine - Small Intestine adenoma	L-arginine-deficient (Harlan Teklad TD 91230)	Intestine - Small Intestine	observed
B6;129P2-Apc^Min/+ Nos2^tm1Lau	Intestine - Small Intestine adenoma		Intestine - Small Intestine	observed
B6;129P2-Apc^Min/+ Nos2^tm1Lau/+	Intestine - Small Intestine adenoma		Intestine - Small Intestine	observed
B6;129P2-Apc^Min/+	Intestine - Small Intestine adenoma		Intestine - Small Intestine	observed