Pathology Image Detail

Caption	Fig. 4D&E "Left panel shows p63 (red) and CK5 (green) fluorescent immunohistochemistry analysis with labelled cells protruding into the lumen (white arrowheads) in a region of PIN in Brca2^F/F;Trp53^F/F;PBCre4 mutants. Right panel shows CK5 (green, basal cells) and CK8 (red, luminal cells) fluorescent immunohistochemistry with PIN lesions in Brca2^F/F;Trp53^F/F;PBCre4 mutants displaying an increase in luminal cells next to clusters of basal cells. White arrowhead marks CK5 and CK8 double-labelled cells. DAPI nuclear stain is blue. The anterior prostates of 16-month-old animals are shown."
Description	immunohistochemistry various markers in PIN from Brca2;Trp53 homozygous mutant mice compared to normal control prostate
Age at Necropsy	16 months
Notes	Fig. 4D&E
Contributor	Francis JC (J:161511)
Pathologist	Francis JC (J:161511)
Copyright	This image is from Francis JC, PLoS Genet 2010;6(6):e1000995, an open-access article distributed under the terms of the Creative Commons Attribution License.
Method	IHC for various markers

Image Probes
Type: antibody	Name anti-keratin 5 (PRB-160P) (Covance) Target mouse keratin 5, C-terminus Supplier Covance Notes Monospecific polyclonal rabbit antibody raised against a peptide sequence derived from the C-terminus of the mouse keratin 5 protein, KFVSTTSSSRRSPKS.
Type: antibody	Name anti-p63 (4A4) (sc-8431) Target amino acids 1-205 at the N-terminus of human p63 Supplier Santa Cruz Biotechnology Notes mouse monoclonal IgG2a; "recommended for detection of p63 variants of m[ouse], r[at] and h[uman] origin"
Type: antibody	Name anti-CK8 (MMS-162P) Target cytokeratin 8 Supplier Covance Notes mouse monoclonal antibody

Model

MMHC ID: MTB:46353

Tumor Name: Prostate gland • prostatic intraepithelial neoplasia - high grade (HGPIN)

Treatment Type: None (spontaneous)

Tumor Synonyms: prostate HG PIN; high-grade prostate intraepithelial neoplasia

Organ Affected: Prostate gland

Frequency: 84%

Reference: J:161511

Strain

Strain: [not specified]-Brca2^tm1Brn Tg(Pbsn-cre)4Prb Trp53^tm1Brn

Strain Types: targeted mutation (conditional); transgenic

General Note: Compound mutant mice. The genetic background of these mice was described as a "mixed genetic background" without the involved strains being specified. It included at least some 129P2/OlaHsd and some C57BL/6 and/or DBA/2.

Strain Synonyms: Brca2^F/F;Trp53^F/F;PBCre4; Brca2;Trp53 homozygous mutant

Strain Sex

Male

Reproductive Status

reproductive status not specified

Age of Onset

12 months

Age of Detection

15-19 months