Reference SummaryNambiar PR, Int J Oncol 2003 Jan;22(1):145-50

Title

Preliminary analysis of azoxymethane induced colon tumors in inbred mice commonly used as transgenic/knockout progenitors.

Authors

Nambiar PR; Girnun G; Lillo NA; Guda K; Whiteley HE; Rosenberg DW

Journal

Int J Oncol

Volume

22

Issue

1

Year

2003

Pages

145-50

Abstract

Azoxymethane (AOM) is a colon carcinogen that is used to study the pathogenesis of sporadic colorectal cancer. We have evaluated differential susceptibility to AOM in inbred mice used as progenitors of recombinant/transgenic lines. In experiment 1, male FVB/N, 129/SvJ, C57Bl/6J mice were treated i.p. with 10 mg/kg AOM once per week for 4 weeks and sacrificed after 20 weeks. Only AOM-treated FVB/N mice developed tumors (3.6 tumors/mouse) in distal colon. In experiment 2, A/J, AKR/J, Balb/CJ mice were treated with AOM for 6 weeks and sacrificed after 24 weeks. AOM-treated A/J and Balb/CJ mice developed 9.2 and 1 tumor/mouse, respectively. Despite these differences, tumors had similar morphology regardless of strain. Immunohistochemistry with beta-catenin resulted in marked nuclear and cytoplasmic staining of tumor cells in FVB/N. However, fainter and heterogeneous beta-catenin staining was observed in A/J tumors, suggesting distinct pathways of tumorigenesis in different strains. Irrespective of cytological features of malignancy, tumor cells rarely breached the muscularis mucosa and showed no evidence of distant metastasis. Lack of invasiveness and metastasis in even the most sensitive strains provides a model system for studying the potential role of 'metastasis genes' in imparting a malignant phenotype.

Links

12469197 – National Library of Medicine/PubMed

Strain Notes

Strain Note
FVB/NJ Mice were purchased from The Jackson Laboratory.

Models

Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
A/J Intestine - Large Intestine - Colon - Distal adenocarcinoma
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

FVB/NJ Intestine - Large Intestine - Colon - Distal adenocarcinoma
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

129X1/SvJ Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

A/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

AKR/J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

BALB/cJ Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

C57BL/6J Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

FVB/NJ Intestine - Large Intestine - Colon - Distal foci - aberrant crypt (ACF)
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

A/J Intestine - Large Intestine - Colon - Distal squamous cell carcinoma
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

observed

FVB/NJ Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

100

129X1/SvJ Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

0

C57BL/6J Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

0

A/J Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

100

AKR/J Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

0

BALB/cJ Intestine - Large Intestine - Colon - Distal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Distal

100

129X1/SvJ Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

A/J Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

AKR/J Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

BALB/cJ Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

C57BL/6J Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0

FVB/NJ Intestine - Large Intestine - Colon - Proximal tumor
  • azoxymethane (AOM)
Intestine - Large Intestine - Colon - Proximal

0