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Pathology Image Detail

Caption	A-J, H&E staining, thyroid sections from WT, p53/Rb DKO, p53/Rb/Pten TKO, and p53/Pten DKO mice at the time points indicated. K-Y, immunostaining of thyroid sections derived from the same strains of mice at the time points indicated.
Description	thyroid lesions from affected mice of multiple genotypes compared to wild-type and unaffected mice; H&E, IHC for ASCL1 and SYP, and Ki67 staining
Age at Necropsy	2-10 months post injection
Notes	Fig. 3A-Y
Contributor	Song H (J:264655)
Pathologist	Song H (J:264655)
Copyright	This image is from Song H, J Biol Chem 2017 Mar 3;292(9):2888-3899 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. J:264655; see full text from http://www.jbc.org/content/292/9/3888.full.
Method	H&E and IHC using various antibodies

Image Probes
Type: antibody	Name anti-Ki-67 (550609) Target human MKI67 Supplier BD Biosciences Notes Monoclonal antibody raised against human MKI67
Type: antibody	Name anti-synaptophysin (Life Technologies, 08-0130) Target synaptophysin Supplier Life Technologies Notes rabbit anti-SYP (prediluted; Life Technologies catalog no. 08-0130)
Type: antibody	Name anti-ASCL1 (MASH1) (556604) Target ASCL1 (MASH1) Supplier BD Biosciences Notes mouse anti-ASCL1 (MASH1) (BD Biosciences catalog no. 556604)

Model

MMHC ID: MTB:96869

Tumor Name: Thyroid gland - Medulla - C cell • carcinoma

Treatment Type: Genetic Modification

Agent: tamoxifen (for inducing expression from mutant alleles)

Tumor Synonyms: medullary thyroid tumor; medullary thyroid carcinoma; thyroid tumor

Organ Affected: Thyroid gland - Medulla - C cell

Frequency: 100%

Frequency Note: 4-7 week old mice were given tamoxifen (0.25 mg/g body weight, in corn oil) i.p. once daily for 3 days.

Reference: J:264655

Strain

Strain: [not specified]-Calca^{tm1.1(cre/ERT2)Ptch}/+ Pten^tm1Hwu Rb1^tm2Brn Trp53^tm1Brn

Strain Types: targeted mutation (conditional); targeted mutation (knock-in)

General Note: Compound mutant mice. The genetic background of these mice was not specified but included at least some 129P2/OlaHsd, 129S4/SvJae, 129 (substrain not specified), BALB/c, FVB, and possibly some other strain(s).

Strain Synonyms: CGRP^CreER/+; p53^f/f; Rb^f/f; Pten^f/f; p53^-/-; Rb^-/-; Pten^-/-; triple knock-out; TKO; p53/Rb/Pten TKO; p53/Rb/Pten-deficient

Strain Sex: Mixed Population

Reproductive Status: reproductive status not specified

Age of Onset: ~2.5-3.5 months

Age of Detection: 3-4 months