Reference SummaryRaafat A, PLoS One 2012;7(8):e43624


Effects of age and parity on mammary gland lesions and progenitor cells in the FVB/N-RC mice.


Raafat A; Strizzi L; Lashin K; Ginsburg E; McCurdy D; Salomon D; Smith GH; Medina D; Callahan R


PLoS One










The FVB/N mouse strain is extensively used in the development of animal models for breast cancer research. Recently it has been reported that the aging FVB/N mice develop spontaneous mammary lesions and tumors accompanied with abnormalities in the pituitary glands. These observations have a great impact on the mouse models of human breast cancer. We have developed a population of inbred FVB/N mice (designated FVB/N-RC) that have been genetically isolated for 20 years. To study the effects of age and parity on abnormalities of the mammary glands of FVB/N-RC mice, twenty-five nulliparous and multiparous (3-4 pregnancies) females were euthanized at 16-22 months of age. Examination of the mammary glands did not reveal macroscopic evidence of mammary gland tumors in either aged-nulliparous or multiparous FVB/N-RC mice (0/25). However, histological analysis of the mammary glands showed rare focal nodules of squamous changes in 2 of the aged multiparous mice. Mammary gland hyperplasia was detected in 8% and 71% of the aged-nulliparous and aged-multiparous mice, respectively. Epithelial contents and serum levels of triiodothyronine were significantly higher in the experimental groups than the 14-wk-old control mice. Immuno-histochemical staining of the pituitary gland pars distalis showed no difference in prolactin staining between the control and the aged mice. Tissue transplant and dilution studies showed no effect of age and/or parity on the ability of putative progenitor cells present among the injected mammary cells to repopulate a cleared fat pad and develop a full mammary gland outgrowth. This FVB/N-RC mouse substrain is suitable to develop mouse models for breast cancer.


J:191662 – MGI References
22952723 – National Library of Medicine/PubMed


Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
FVB/NRnc Mammary gland hyperplasia Mammary gland

0 - 40

FVB/NRnc Mammary gland hyperplasia - alveolar Mammary gland

0 - observed

FVB/NRnc Mammary gland metaplasia - squamous Mammary gland

0 - observed

FVB/NRnc Mammary gland tumor Mammary gland


FVB/NRnc Pituitary gland - Pars distalis adenoma Pituitary gland - Pars distalis


FVB/NRnc Pituitary gland - Pars distalis cyst Pituitary gland - Pars distalis

0 - 50

FVB/NRnc Uterus cyst Uterus

0 - 20