Reference SummarySheldon WG, Toxicol Pathol 1995 Jul-Aug;23(4):458-76

Title

 Age-related neoplasia in a lifetime study of ad libitum-fed and food-restricted B6C3F1 mice.

Authors

 Sheldon WG; Bucci TJ; Hart RW; Turturro A

Journal

 Toxicol Pathol

Volume

 23

Issue

 4

Year

 1995

Pages

 458-76

Abstract

 Longevity, body weight, and age-specific neoplasia were determined in 1,064 B6C3F1 mice as part of a coordinated study of food restriction (FR). Restricted animals were offered 60% of the diet consumed by the ad libitum (AL) group. Longevity data were derived from a set of 56 animals of each sex from each diet group, which were examined whenever dead or moribund. For cross-sectional data, a parallel set of 210 animals were sacrificed in groups of 12-15 at 6-mo intervals. Lifetime body weight was reduced in the FR mice approximately proportional to restriction (i.e., 40%). Food restriction increased the age at 50% survival (median) by 36% in both sexes and increased the maximal lifespan (mean age of oldest 10%) by 21.5% in males and by 32.5% in females. In 56 males of the longevity groups, there were 89 neoplasms in the AL subgroup versus 53 in FR; 56 AL females had 100, versus 58 in 55 FR females. Increase in lifespan of the restricted animals was achieved primarily by decrease in incidence and delay of onset of fatal tumors, of which lymphoma was the most prominent. This report catalogs all of the neoplasms (1,103) observed in longevity and cross-sectional groups, by diet, sex, and age. These data add to the existing knowledge base needed for future studies of dietary restriction and aging as well to evaluate nutrition of animals used in bioassays.

Links

 J:31053 – MGI References
7501958 – National Library of Medicine/PubMed

Strain Notes

Strain Note
(C57BL/6N x C3H/HeN)F1 These mice were "produced by the NCTR specific pathogen free (SPF) breeding colony".

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
(C57BL/6N x C3H/HeN)F1 Adipose tissue liposarcoma
  • calorie restricted
 Skin

33.3
(C57BL/6N x C3H/HeN)F1 Adrenal gland - Cortex adenoma Adrenal gland - Cortex

1.4 - 12.5
(C57BL/6N x C3H/HeN)F1 Adrenal gland - Cortex adenoma
  • calorie restricted
 Adrenal gland - Cortex

2.3 - 6.7
(C57BL/6N x C3H/HeN)F1 Adrenal gland - Medulla pheochromocytoma Adrenal gland - Medulla

4.5 - 14.3
(C57BL/6N x C3H/HeN)F1 Adrenal gland - Medulla pheochromocytoma
  • calorie restricted
 Adrenal gland - Medulla

2.5 - 13.3
(C57BL/6N x C3H/HeN)F1 Blood vessel neoplasm Blood vessel

0 - 31.25
(C57BL/6N x C3H/HeN)F1 Blood vessel neoplasm
  • calorie restricted
 Blood vessel

0 - 100
(C57BL/6N x C3H/HeN)F1 Bone osteosarcoma Bone

3.1 - 50
(C57BL/6N x C3H/HeN)F1 Bone osteosarcoma
  • calorie restricted
 Bone

2.5 - 33.3
(C57BL/6N x C3H/HeN)F1 CNS - Brain - Oligodendroglial cell oligodendroglioma CNS - Brain - Oligodendroglial cell

6.25
(C57BL/6N x C3H/HeN)F1 Connective tissue - Fibroblast fibrosarcoma Skin

3.1 - 12.5
(C57BL/6N x C3H/HeN)F1 Connective tissue - Fibroblast fibrosarcoma
  • calorie restricted
 Skin

13.3
(C57BL/6N x C3H/HeN)F1 Ear - Zymbal gland carcinoma Ear - Zymbal gland

7.1
(C57BL/6N x C3H/HeN)F1 Eye - Harderian gland adenocarcinoma Eye - Harderian gland

1.4 - 5.9
(C57BL/6N x C3H/HeN)F1 Eye - Harderian gland adenoma Eye - Harderian gland

3.1 - 35.7
(C57BL/6N x C3H/HeN)F1 Eye - Harderian gland adenoma
  • calorie restricted
 Eye - Harderian gland

2.9 - 23.1
(C57BL/6N x C3H/HeN)F1 Eye - Harderian gland carcinoma Eye - Harderian gland

4.8
(C57BL/6N x C3H/HeN)F1 Eye - Harderian gland neoplasm Eye - Harderian gland

0 - 35.7
(C57BL/6N x C3H/HeN)F1 Eye - Harderian gland neoplasm
  • calorie restricted
 Eye - Harderian gland

0 - 23.1
(C57BL/6N x C3H/HeN)F1 Intestine - Large Intestine carcinoma Intestine - Large Intestine

12.5
(C57BL/6N x C3H/HeN)F1 Intestine - Large Intestine polyp Intestine - Large Intestine

12.5
(C57BL/6N x C3H/HeN)F1 Intestine - Small Intestine polyp Intestine - Small Intestine

1.4
(C57BL/6N x C3H/HeN)F1 Intestine - Small Intestine polyp
  • calorie restricted
 Intestine - Small Intestine

3.8 - 10
(C57BL/6N x C3H/HeN)F1 Kidney - Cortex adenocarcinoma
  • calorie restricted
 Kidney - Cortex

2.5 - 3.8
(C57BL/6N x C3H/HeN)F1 Kidney - Cortex adenoma
  • calorie restricted
 Kidney - Cortex

2.5 - 23.1
(C57BL/6N x C3H/HeN)F1 Kidney - Cortex adenoma Kidney - Cortex

14.3
(C57BL/6N x C3H/HeN)F1 Kidney neoplasm Kidney

0 - 14.3
(C57BL/6N x C3H/HeN)F1 Kidney neoplasm
  • calorie restricted
 Kidney

0 - 25
(C57BL/6N x C3H/HeN)F1 Leukocyte - Monocyte - Macrophage - Histiocyte histiocytic sarcoma Leukocyte - Monocyte - Macrophage - Histiocyte

0 - 28.6
(C57BL/6N x C3H/HeN)F1 Leukocyte - Monocyte - Macrophage - Histiocyte histiocytic sarcoma
  • calorie restricted
 Leukocyte - Monocyte - Macrophage - Histiocyte

0 - 44.4
(C57BL/6N x C3H/HeN)F1 Leukocyte - Myelocyte (Granulocyte) - Basophil - Mast cell tumor
  • calorie restricted
 Leukocyte - Myelocyte (Granulocyte) - Basophil - Mast cell

33.3
(C57BL/6N x C3H/HeN)F1 Leukocyte - Myelocyte (Granulocyte) leukemia Leukocyte - Myelocyte (Granulocyte)

12.5
(C57BL/6N x C3H/HeN)F1 Leukocyte - Myelocyte (Granulocyte) leukemia
  • calorie restricted
 Leukocyte - Myelocyte (Granulocyte)

50
(C57BL/6N x C3H/HeN)F1 Leukocyte lymphoma Leukocyte

0 - 84.2
(C57BL/6N x C3H/HeN)F1 Leukocyte lymphoma
  • calorie restricted
 Leukocyte

0 - 100
(C57BL/6N x C3H/HeN)F1 Liver - Sinusoid - Ito cell neoplasm Liver - Sinusoid - Ito cell

6.7
(C57BL/6N x C3H/HeN)F1 Liver hepatocellular adenoma Liver

4.1 - 50
(C57BL/6N x C3H/HeN)F1 Liver hepatocellular adenoma
  • calorie restricted
 Liver

2.3 - 20
(C57BL/6N x C3H/HeN)F1 Liver hepatocellular carcinoma Liver

5.0 - 100
(C57BL/6N x C3H/HeN)F1 Liver hepatocellular carcinoma
  • calorie restricted
 Liver

2.9 - 30
(C57BL/6N x C3H/HeN)F1 Liver hepatocellular neoplasm Liver

12.0 - 35.9
(C57BL/6N x C3H/HeN)F1 Liver hepatocellular neoplasm
  • calorie restricted
 Liver

6.5 - 6.8
(C57BL/6N x C3H/HeN)F1 Liver neoplasm Liver

0 - 53.3
(C57BL/6N x C3H/HeN)F1 Liver neoplasm
  • calorie restricted
 Liver

0 - 33.3
(C57BL/6N x C3H/HeN)F1 Lung adenoma Lung

4.5 - 42.9
(C57BL/6N x C3H/HeN)F1 Lung adenoma
  • calorie restricted
 Lung

5.0 - 33.3
(C57BL/6N x C3H/HeN)F1 Lung carcinoma Lung

3.1 - 50
(C57BL/6N x C3H/HeN)F1 Lung carcinoma
  • calorie restricted
 Lung

1.9 - 11.1
(C57BL/6N x C3H/HeN)F1 Lung neoplasm Lung

0 - 71.4
(C57BL/6N x C3H/HeN)F1 Lung neoplasm
  • calorie restricted
 Lung

0 - 33.3
(C57BL/6N x C3H/HeN)F1 Mammary gland adenocarcinoma Mammary gland

0 - 35.7
(C57BL/6N x C3H/HeN)F1 Mammary gland adenocarcinoma
  • calorie restricted
 Mammary gland

0 - 5.3
(C57BL/6N x C3H/HeN)F1 Mesodermal cell/mesoblast sarcoma Skin

2.1 - 18.2
(C57BL/6N x C3H/HeN)F1 Mesodermal cell/mesoblast sarcoma
  • calorie restricted
 Liver

6.7
(C57BL/6N x C3H/HeN)F1 Mesodermal cell/mesoblast sarcoma
  • calorie restricted
 Skin

6.7 - 33.3
(C57BL/6N x C3H/HeN)F1 Mesodermal cell/mesoblast sarcoma Abdominal cavity

2.1
(C57BL/6N x C3H/HeN)F1 Mesodermal cell/mesoblast sarcoma Gallbladder

2.9
(C57BL/6N x C3H/HeN)F1 Mesodermal cell/mesoblast sarcoma - poorly differentiated Muscle - Striated - Skeletal

1.4
(C57BL/6N x C3H/HeN)F1 Mesodermal cell/mesoblast sarcoma - poorly differentiated
  • calorie restricted
 Muscle - Striated - Skeletal

2.5 - 5.3
(C57BL/6N x C3H/HeN)F1 Mesothelium mesothelioma Abdominal cavity

5.3
(C57BL/6N x C3H/HeN)F1 Muscle - Smooth leiomyoma Uterus

1.4 - 7.7
(C57BL/6N x C3H/HeN)F1 Muscle - Smooth leiomyosarcoma Intestine

3.1
(C57BL/6N x C3H/HeN)F1 Muscle - Smooth leiomyosarcoma Uterus

1.4 - 10.5
(C57BL/6N x C3H/HeN)F1 Muscle - Smooth leiomyosarcoma
  • calorie restricted
 Uterus

6.7 - 33.3
(C57BL/6N x C3H/HeN)F1 Muscle - Striated - Skeletal rhabdomyosarcoma Muscle - Striated - Skeletal

1.4 - 5.9
(C57BL/6N x C3H/HeN)F1 Nose squamous cell carcinoma
  • calorie restricted
 Nose

3.7
(C57BL/6N x C3H/HeN)F1 Ovary - Germ cell teratoma
  • calorie restricted
 Ovary - Germ cell

7.1 - 50
(C57BL/6N x C3H/HeN)F1 Ovary - Granulosa cell tumor Ovary - Granulosa cell

3.1 - 10.5
(C57BL/6N x C3H/HeN)F1 Ovary - Granulosa cell tumor
  • calorie restricted
 Ovary - Granulosa cell

6.7 - 14.3
(C57BL/6N x C3H/HeN)F1 Ovary adenoma - cystic papillary Ovary

1.4 - 7.1
(C57BL/6N x C3H/HeN)F1 Ovary adenoma - cystic papillary
  • calorie restricted
 Ovary

14.3
(C57BL/6N x C3H/HeN)F1 Ovary adenoma - tubular
  • calorie restricted
 Ovary

2.9
(C57BL/6N x C3H/HeN)F1 Ovary luteoma Ovary

3.1 - 5.3
(C57BL/6N x C3H/HeN)F1 PNS - Ganglion - Paraganglion chemodectoma PNS - Ganglion - Paraganglion

14.3
(C57BL/6N x C3H/HeN)F1 Pancreas - Islet of Langerhans adenoma Pancreas - Islet of Langerhans

4.5 - 4.8
(C57BL/6N x C3H/HeN)F1 Pancreas - Islet of Langerhans adenoma
  • calorie restricted
 Pancreas - Islet of Langerhans

2.3 - 6.7
(C57BL/6N x C3H/HeN)F1 Pancreas - Islet of Langerhans carcinoma
  • calorie restricted
 Pancreas - Islet of Langerhans

2.3 - 3.8
(C57BL/6N x C3H/HeN)F1 Parathyroid gland adenoma Parathyroid gland

6.25
(C57BL/6N x C3H/HeN)F1 Penis squamous cell carcinoma
  • calorie restricted
 Penis

3.8
(C57BL/6N x C3H/HeN)F1 Peritoneum - Mesentery neoplasm - malignant - anaplastic Abdominal cavity

3.1
(C57BL/6N x C3H/HeN)F1 Pituitary gland - Pars distalis adenoma Pituitary gland - Pars distalis

0 - 100
(C57BL/6N x C3H/HeN)F1 Pituitary gland - Pars distalis adenoma
  • calorie restricted
 Pituitary gland - Pars distalis

0 - 13.3
(C57BL/6N x C3H/HeN)F1 Skin - Hair follicle - Basal cell trichoepithelioma Skin - Hair follicle - Basal cell

5.3
(C57BL/6N x C3H/HeN)F1 Skin carcinoma - basosquamous Skin

2.7 - 14.3
(C57BL/6N x C3H/HeN)F1 Skin squamous cell carcinoma
  • calorie restricted
 Skin

5.0
(C57BL/6N x C3H/HeN)F1 Stomach adenocarcinoma
  • calorie restricted
 Stomach

10
(C57BL/6N x C3H/HeN)F1 Stomach papilloma Stomach

2.9 - 100
(C57BL/6N x C3H/HeN)F1 Stomach papilloma
  • calorie restricted
 Stomach

6.7
(C57BL/6N x C3H/HeN)F1 Stomach squamous cell carcinoma Stomach

6.7 - 14.3
(C57BL/6N x C3H/HeN)F1 Stomach squamous cell carcinoma
  • calorie restricted
 Stomach

6.7
(C57BL/6N x C3H/HeN)F1 Testis - Leydig cell (Interstitial cell) adenoma Testis - Leydig cell (Interstitial cell)

6.25
(C57BL/6N x C3H/HeN)F1 Testis - Leydig cell (Interstitial cell) adenoma
  • calorie restricted
 Testis - Leydig cell (Interstitial cell)

7.7
(C57BL/6N x C3H/HeN)F1 Thyroid gland - Follicular cell adenoma Thyroid gland - Follicular cell

0 - 29.4
(C57BL/6N x C3H/HeN)F1 Thyroid gland - Follicular cell adenoma
  • calorie restricted
 Thyroid gland - Follicular cell

0 - 6.7
(C57BL/6N x C3H/HeN)F1 Thyroid gland - Medulla - C cell carcinoma
  • calorie restricted
 Thyroid gland - Medulla - C cell

5.0 - 33.3
(C57BL/6N x C3H/HeN)F1 Thyroid gland neoplasm Thyroid gland

0 - 12.0
(C57BL/6N x C3H/HeN)F1 Thyroid gland neoplasm
  • calorie restricted
 Thyroid gland

0 - 2.6
(C57BL/6N x C3H/HeN)F1 Tongue squamous cell carcinoma Tongue

3.1 - 7.1
(C57BL/6N x C3H/HeN)F1 Uterus polyp Uterus

1.4 - 21.4
(C57BL/6N x C3H/HeN)F1 Uterus polyp
  • calorie restricted
 Uterus

2.9
(C57BL/6N x C3H/HeN)F1 Vagina squamous cell carcinoma
  • calorie restricted
 Vagina

6.7
(C57BL/6N x C3H/HeN)F1 (Unspecified organ) neoplasm (Unspecified organ)

0 - 100
(C57BL/6N x C3H/HeN)F1 (Unspecified organ) neoplasm
  • calorie restricted
 (Unspecified organ)

0 - 100
(C57BL/6N x C3H/HeN)F1 (Unspecified organ) neoplasm - malignant - anaplastic (Unspecified organ)

observed