Reference SummaryElkin M, Carcinogenesis 1998 Dec;19(12):2095-9


The dynamics of the imprinted H19 gene expression in the mouse model of bladder carcinoma induced by N-butyl-N-(4-hydroxybutyl)nitrosamine.


Elkin M; Ayesh S; Schneider T; de Groot N; Hochberg A; Ariel I












The imprinted H19 gene product is an oncofetal RNA molecule in humans. It is expressed in fetal bladder, down-regulated postnatally and is re-expressed in human bladder carcinoma. This study was designed to investigate the dynamics of the expression of H19 in the mouse bladder carcinoma induced by N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) and its relation to stages of neoplastic transformation. BBN was administered to mice in the drinking water for 26-28 weeks. The bladders were removed at 5-10 week intervals for histopathological examination and for in situ hybridization for H19 RNA, using a 35S-labeled probe. Following BBN administration expression of H19 first appeared after 5 weeks in the lamina propria adjacent to the basement membrane, concomitant with mucosal hyperplasia. At 11 weeks focal expression was noted in epithelial cells. Invasive carcinomas, of the transitional and squamous sub-types, were seen after 20 weeks and more of BBN administration. At this stage H19 expression was observed in scattered tumor cells, in the connective tissue stroma of the tumor and in the lamina propria underlying the remaining hyperplastic/dysplastic mucosa. Abundant expression of H19 was evident in fetal bladder but was absent in normal adult bladder. We conclude that, similar to humans, the H19 gene product is an oncofetal RNA molecule in the experimental mouse model of bladder carcinoma. In this model H19 is expressed in the connective tissue of the lamina propria prior to its expression in epithelial cells, concurrent with preneoplastic changes in the transitional epithelium of the bladder.


J:51847 – MGI References
9886562 – National Library of Medicine/PubMed

Strain Notes

Strain Note
C3H/He After 5 or more weeks of treatment with BBN the wild type allele of the H19 gene was reported to be expressed in the bladder of these mice.
Expression of the wild type allele of the H19 gene was reported to not be detected in the bladder of these mice.


Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
C3H/He Urinary bladder carcinoma
  • N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Urinary bladder

0 - 82

C3H/He Urinary bladder carcinoma Urinary bladder


C3H/He Urinary bladder dysplasia
  • N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Urinary bladder

observed - 100

C3H/He Urinary bladder hyperplasia
  • N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Urinary bladder


C3H/He Urinary bladder hyperplasia - nodular
  • N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Urinary bladder

55 - 100

C3H/He Urinary bladder metaplasia - squamous
  • N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Urinary bladder


C3H/He Urinary bladder squamous cell carcinoma
  • N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Urinary bladder

9.1 - 50

C3H/He Urinary bladder transitional cell carcinoma
  • N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)
Urinary bladder