Reference SummaryThompson JA, Int J Cancer 1997 Jul 3;72(1):197-202

Title

 Expression of transgenic carcinoembryonic antigen (CEA) in tumor-prone mice: an animal model for CEA-directed tumor immunotherapy.

Authors

 Thompson JA; Eades-Perner AM; Ditter M; Muller WJ; Zimmermann W

Journal

 Int J Cancer

Volume

 72

Issue

 1

Year

 1997

Pages

 197-202

Abstract

 Carcinoembryonic antigen (CEA) is a tumor marker for the most common forms of adenocarcinomas, We have previously described C57BL/6 mice transgenic for the complete human CEA gene, Compared with humans, these mice reveal a conserved spatiotemporal CEA expression pattern, To establish animal models for CEA-targeted tumor immunotherapy, we have crossed CEA transgenic mice with mice that are genetically predisposed to tumor development, These immunocompetent animals should allow optimization of immunotherapy strategies for maximal destruction of tumor tissues with minimal damage to CEA- expressing normal tissues, To develop a breast tumor model, CEA transgenic mice were cross-bred with mice transgenic for the rat neo protooncogene controlled by the mouse mammary tumor virus long terminal repeat, Female offspring developed poorly differentiated breast tumors, none of which, however, expressed CEA, As a model for colorectal tumors, mice bearing a mutation in the Ape gene (Min mice) and the CEA transgene developed multiple intestinal adenomas with strong CEA expression in all tumor cells, CEA expression had no significant effect on tumor growth, Occasional, well-differentiated breast adenocarcinomas in female offspring expressed CEA focally in tumor cells lining pseudolumina, Cross-breeding Apc(Min/+) mice with neu transgenic mice did not reveal a synergistic effect on the kinetics of breast tumor formation, Finally, CEA transgenic mice crossbred with mice transgenic for the SV40 large T antigen regulated by the surfactant protein-C promoter, developed multiple lung adenocavcinomas that revealed a mosaic CEA expression pattern. (C) 1997 Wiley-Liss, Inc.

Links

 J:41380 – MGI References
9212243 – National Library of Medicine/PubMed

Strain Notes

Strain Note
STOCK Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-ApcMin/J mice purchased from The Jackson Laboratory.
STOCK ApcMin/+ Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-ApcMin/J mice purchased from The Jackson Laboratory.
STOCK ApcMin/+ Tg(CEACAM5)#Wzm Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-ApcMin/J mice purchased from The Jackson Laboratory.
STOCK Tg(CEACAM5)#Wzm Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with C57BL/6J-ApcMin/J mice purchased from The Jackson Laboratory.
STOCK Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with FVB/N-Tg(MMTVneu)202Mul mice.
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with FVB/N-Tg(SFTPC-TAg)5Jaw mice provided by Dr. J. Whitsett at the Children's Hospital Medical Center, Cincinatti, OH.
STOCK Tg(SFTPC-TAg)5.1Jaw Mice were generated by mating Tg(CEACAM5)#Wzm mice from 1st or 2nd filial backcross onto C57BL/6 with FVB/N-Tg(SFTPC-TAg)5Jaw mice provided by Dr. J. Whitsett at the Children's Hospital Medical Center, Cincinatti, OH.

Models
Strain Model Name Treatment Agent(s) Organ Affected Frequency Model Details
STOCK ApcMin/+ Tg(CEACAM5)#Wzm Intestine - Large Intestine - Colon adenoma Intestine - Large Intestine - Colon

observed
STOCK ApcMin/+ Intestine - Large Intestine - Colon adenoma Intestine - Large Intestine - Colon

observed
STOCK Tg(CEACAM5)#Wzm Intestine - Large Intestine - Colon adenoma Intestine - Large Intestine - Colon

0
STOCK Intestine - Large Intestine - Colon adenoma Intestine - Large Intestine - Colon

0
STOCK ApcMin/+ Tg(CEACAM5)#Wzm Intestine - Small Intestine adenoma Intestine - Small Intestine

observed
STOCK ApcMin/+ Intestine - Small Intestine adenoma Intestine - Small Intestine

observed
STOCK Tg(CEACAM5)#Wzm Intestine - Small Intestine adenoma Intestine - Small Intestine

0
STOCK Intestine - Small Intestine adenoma Intestine - Small Intestine

0
STOCK ApcMin/+ Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul Intestine adenoma Intestine

observed
C57BL/6J-ApcMin/+ Intestine adenoma Intestine

observed
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw Lung adenocarcinoma Lung

observed
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw Lung adenoma Lung

observed
STOCK Tg(SFTPC-TAg)5.1Jaw Lung adenoma Lung

observed
STOCK Tg(CEACAM5)#Wzm Tg(SFTPC-TAg)5.1Jaw Lung hyperplasia Lung

observed
STOCK ApcMin/+ Mammary gland adenoma Mammary gland

observed
STOCK ApcMin/+ Tg(CEACAM5)#Wzm Mammary gland tumor Mammary gland

18
STOCK Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul Mammary gland tumor Mammary gland

observed
STOCK ApcMin/+ Tg(CEACAM5)#Wzm Tg(MMTVneu)202Mul Mammary gland tumor Mammary gland

observed