Reference SummaryChen B, Carcinogenesis 1994 Sep;15(9):2031-5
Title |
Allele-specific activation and expression of the K-ras gene in hybrid mouse lung tumors induced by chemical carcinogens. | ||||
Authors |
Chen B; You L; Wang Y; Stoner GD; You M | ||||
Journal |
Carcinogenesis | ||||
Volume |
15 | ||||
Issue |
9 | ||||
Year |
1994 | ||||
Pages |
2031-5 | ||||
Abstract |
A mouse hybrid, (C3H x A/J)F1 or C3A, was developed by crossing male A/J mice (high lung tumor susceptibility) with female C3H mice (low lung tumor susceptibility). The lung tumor responses of the C3A mice to dimethylnitrosamine (DMN) or benzo[a]pyrene (B[a]P) were found to be intermediate between those of the two parental strains. Mutational activation of the K-ras gene was found at a high frequency in both the B[a]P- and DMN-induced C3A lung tumors. To explore the genetic basis of the K-ras gene involvement in mouse lung tumor susceptibility, the parental origin of the K-ras oncogene in the chemically induced C3A lung tumors was determined. K-ras oncogenes were found on the allele inherited from the susceptible A/J parent in 14/16 of DMN-induced tumors and 15/17 of B[a]P-induced tumors from C3A mice. Furthermore, the K-ras mRNA transcribed from the A/J allele was 5-20 times more than C3H K-ras transcripts in 10/10 DMN-induced and 10/10 B[a]P-induced C3A lung tumors. These data suggest that an activated A/J K-ras allele could be more tumorigenic than an activated C3H allele due to the differential expression of the two alleles in lung cells. | ||||
Links |
J:20674 – MGI References 7923598 – National Library of Medicine/PubMed |
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Strain Notes
|
Strain | Model Name | Treatment Agent(s) | Organ Affected | Frequency | Model Details |
---|---|---|---|---|---|
(C3H/HeJ x A/J)F1 | Lung adenoma |
|
Lung |
100 |
|
(C3H/HeJ x A/J)F1 | Lung adenoma |
|
Lung |
85 |
|
(C3H/HeJ x A/J)F1 | Lung adenoma | Lung |
0 - 11 |
||
A/J | Lung adenoma |
|
Lung |
100 |
|
A/J | Lung adenoma |
|
Lung |
95 |
|
A/J | Lung adenoma | Lung |
25 - 42 |
||
C3H/HeJ | Lung adenoma |
|
Lung |
10 |
|
C3H/HeJ | Lung adenoma | Lung |
0 |