Lymphoma Pathology
CASE # Strain Diagnosis Immunostain Type of Slide Fixative NOTES      
31106-A NFS.V Follicular lymphoma, spleen Histology 10% NBF
31106-A CD3+ Immunohistochemistry 1:400 antigen retrieval
31106-A BIOT.CD45R+++ Immunohistochemistry 1:200 antigen retrieval
99000951-5 B6;129 Follicular lymphoma, spleen Histology 10% NBF
99000951-5 CD45R+++ Immunohistochemistry BIO 1:100 antigen retrieval
99000951-5 CD3++ Immunohistochemistry 1:200 antigen retrieval
99000951-8A B6;129 Follicular lymphoma, mesenteric lymph nodes and intestine (Peyer's patches)   Histology 10% NBF        
00000092-8A B6;129 Follicular lymphoma, with focalhistiocytic sarcoma, mesenteric lymph nodes Histology 10% NBF
00000092-8A CD45R+++ Immunohistochemistry 1:200 antigen retrieval
00000092-8A CD3++ Immunohistochemistry 1:400 antigen retrieval
00000092-5 B6;129 Follicular lymphoma, with focal histiocytic sarcoma, spleen   Histology 10%NBF        
00000092-5     CD3++ Immunohistochemistry   1:200 antigen retrieval      
00000381-8A B6;129 Follicular lymphoma, pancreatic-duodenal lymph node Histology 10%NBF
00000381-8A BIOT.CD45R+++ Immunohistochemistry 1:200 antigen retrieval
00000381-8A CD3++ Immunohistochemistry 1:400 antigen retrieval
00000381-5 B6;129 Folicular lymphoma, spleen Histology
32525-A NFS.V Plasma cell lymphoma, anaplastic, spleen Histology 10%NBF
30923-A NFS.V Immunoblastic lymphoma (diffuse large B-cell lymphoma), spleen   Histology 10%NBF        
29999A NFS.V Diffuse large B-cell lymphoma, centroblastic, spleen Histology 10%NBF
29999A CD3+ Immunohistochemistry 1:400 antigen retrieval
29999A BIOT.CD45R+++ Immunohistochemistry 1:200 antigen retrieval
32356A NFS.V Diffuse large B-cell lymphoma, centroblastic, histiocyte-rich, spleen, lymph nodes   Histology          
31213 NFS.V Diffuse large B-cell lymphoma, centroblastic, histiocyte-rich, spleen   Histology          
26490-A NFS.V Lymphoma, lymphoblastic, B-cell, lymph nodes Histology 10%NBF
26490-A CD3-, lung CD3+++ Immunohistochemistry 1:400 antigen retrieval
26490-A CD45R+++ Immunohistochemistry 1:200 antigen retrieval
98005791-2A B6;129-Trp53<sup>Tm1Brd</sup> Marginal zone hyperplasia, spleen Histology BOUINS
32801 NFS.V Marginal zone lymphoma, spleen Histology
32801 BIOT.CD45R+++ Immunohistochemistry 1:200 antigen retrieval
32801 CD3- Immunohistochemistry 1:400 antigen retrieval
98005351-1A B6;129-Trp53<sup>Tm1Brd</sup> Marginal zone lymphoma, spleen, advanced Histology BOUINS
35599   Marginal zone lymphoma, spleen   Histology          
35200 NFS.V lymphoblastic lymphoma   Histology   1:200 antigen retrieval      
98005218-2 B6;129-Trp53<sup>Tm1Brd</sup> Lymphoblastic lymphoma, thymic origin, early Histology 10%NBF
98005218-2 CD3 (DAKO)+++ Immunohistochemistry 1:50
98105402-5 B6;129 Lymphoblastic lymphoma, thymic T-cell origin, spleen, liver Histology 10%NBF
98105402-5 CD3+++ Immunohistochemistry 1:400 antigen retrieval
00007622-1 B6;129 Histiocytic sarcoma, liver Histology 10%NBF
00007622-1 CD68+++ Immunohistochemistry 1:2,000
00007622-1 F4/80+++ Immunohistochemistry 1:25 37C rat elite kit
8680 FVB/N-Tg(PML-RARA)556Kog Myeloid leukemia, preleukemic, spleen Histology BOUINS
8680     INOS Immunohistochemistry BOUINS 1:2000, ON4 antigen retrieval CITR. BUFFER  
35775 CFW Myeloid leukemia, undifferentiated, without maturation, spleen, liver, lymph node   Histology          
27836-A NFS.V Erythroid leukemia, spleen, liver Histology 10%NBF
35311 B6;129-Trp53<sup>Tm1Brd</sup> Lymphoblastic lymphoma, thymic T-cell origin and marginal zone lymphoma, spleen Histology
35311 CD3+++ Immunohistochemistry 1:400 antigen retrieval
35311 BIOT.CD45R- Immunohistochemistry 1:100 antigen retrieval
35311 TDT Immunohistochemistry 1:200 antigen retrieval EDTA
CD3 NORMAL Control
CD45R NORMAL Control
CASE # link connects to a Pathology Image Detail page for that sample containing details for that image and a link to a Whole Slide Scan of that image.
Study set created by Herbert C. Morse III (NIAID, NIH), Torgny Fredrickson (NIAID, NIH), and Jerrold M. Ward (NCI, NIH) in 2001.
This work was supported in part with funds from the Mouse Model of Cancers Consortium, National Cancer Institute, National Institutes of health, and by contract N01-C056000 from the National cancer Institute.
References
Campo, E., et al., 2011. The 2008 WHO classification of lymphoid neoplasms and beyond: evolving concepts and practical applications. Blood 117: 50195032.
Kogan, S. C., et al., 2002. Bethesda proposals for classification of nonlymphoid hematopoietic neoplasms in mice. Blood. 100, 238-45.
HC Morse III, J.M. Ward and M.A. Teitell. Mouse models of human B lymphoid neoplasms, IN: I. Magrath, editor, The Lymphoid Neoplasms, 3ed,  London: Hodder Arnold, p 281-292, 2010.
Morse, H. C. I., et al., Mouse models of human B lymphoid neoplasms. In: I. Magrath, et al., Eds.), The Lymphoid Neoplasms. Hodder Arnold, London, 2010, pp. 281-292.
Morse, H. C., 3rd, et al., 2003. B lymphoid neoplasms of mice: characteristics of naturally occurring and engineered diseases and relationships to human disorders. Adv Immunol. 81, 97-121.
Morse, H. C., 3rd, et al., 2002. Bethesda proposals for classification of lymphoid neoplasms in mice. Blood. 100, 246-58.
Rehg, J. E., et al., 2012. The utility of immunohistochemistry for the identification of hematopoietic and lymphoid cells in normal tissues and interpretation of proliferative and inflammatory lesions of mice and rats. Toxicol Pathol. 40, 345-74.
Ward, J. M., 2006. Lymphomas and leukemias in mice. Exp Toxicol Pathol. 57, 377-81.